LOCUS       sequence-88774-e        1822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1822)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-H2B-6
FEATURES             Location/Qualifiers
     source          1..1822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..389
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             402..1079
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1128..1805
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gtaccgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga
       61 aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga
      121 gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt
      181 ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg
      241 aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga
      301 cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta
      361 aggccatcac caagtacacc agcgctaagg atccaggtac catgagtgcg attaagccag
      421 acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
      481 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg
      541 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
      601 tattcgccga atatccagac cacatacaag actattttaa gcagtcgttt cctaaggggt
      661 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca
      721 taacaatgga aggggacact ttctataata aagttcgatt tcacggtgta aactttcccg
      781 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
      841 atgtgcgtga tggagtgctg accggtgata ttcgcatggc tttgttgctt gaaggaaatg
      901 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
      961 caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca
     1021 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgag
     1081 gatccagcgg tcacggtact ggttctactg gttctggttc ttctgagagt gcgattaagc
     1141 cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
     1201 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
     1261 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
     1321 gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg
     1381 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg
     1441 acataacaat ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc
     1501 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
     1561 tgtatgtgcg tgatggagtg ctgaccggtg atattcgcat ggctttgttg cttgaaggaa
     1621 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
     1681 taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
     1741 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
     1801 gataagcggc cgcgactcta ga
//
