LOCUS       sequence-88775-e        1854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1854)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..706
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             755..1429
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             1460..1837
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa
       61 cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
      121 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
      181 ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata
      241 tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
      301 aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg
      361 ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt
      421 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
      481 agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg
      541 tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt
      601 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
      661 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg gtagcggtca
      721 cggtactggt tctactggtt ctggttcttc tgagagtgcg attaagccag acatgaagat
      781 caacctccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
      841 aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct
      901 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccga
      961 atatccagac cacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
     1021 acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga
     1081 aggggacact ttctataata aagttcgatt tcacggtgta aactttcccg ccaatggtcc
     1141 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
     1201 tggagtgctg actggtgata ttcgcatggc tttgttgctt gaaggaaatg cccattaccg
     1261 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca
     1321 ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct
     1381 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat ccggactcag
     1441 atctggaggc tccggaggcc cagagccagc gaagtctgct cccgccccga aaaagggctc
     1501 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
     1561 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
     1621 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
     1681 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
     1741 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
     1801 tactaaggcc atcaccaagt acaccagcgc taagtaagga tccaccggat ctag
//
