LOCUS       sequence-8878-ex        2194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Caspase-3 FRET biosensor, mammalian expression of 
            mAmetrine-DEVD-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: pmAmetrine-DEVD-tdTomato
FEATURES             Location/Qualifiers
     source          1..2194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(68..89)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             758..2188
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
       61 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga
      121 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca cctccggcaa
      181 gctgcccgtg ccctggccca ccctcgtgac caccctgtct tacggcgtgc agtgcttcgc
      241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      301 cgtccaggag cgcaccatct ccttcaagga cgacggcagc tacaggaccc gcgccgaggt
      361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      421 ggacggcaac atcctggggc acaagctgga gtacaacatg aacgtgtggg acgcgtatat
      481 cacggccgac aagcagaaga acggcatcaa agcgaacttc aagatcgagc acaacgtcga
      541 ggacggcggc gtgcagctcg ccgacgcgta ccagcagaac acccccatcg gcgacggctc
      601 cgtgctgctg cctgacaacc actacctgag cttccagagc aagctgttca aagaccccaa
      661 cgagcagcgc gatcacatgg tcctgctgga gttcgttacc gccgccggga tcactctcgg
      721 cggtaccggc agtggcagcg gagatgaggt cgatggtatg gtgagcaagg gcgaggaggt
      781 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
      841 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      901 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
      961 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     1021 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     1081 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     1141 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     1201 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     1261 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     1321 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     1381 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
     1441 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
     1501 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     1561 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1621 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     1681 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     1741 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     1801 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     1861 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1921 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     1981 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     2041 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     2101 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     2161 gtacggcatg gacgagctgt acaagtaagg atcc
//
