LOCUS       sequence-88780-e        3890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3890)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Integrin-Beta3-N-18
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2448..3125
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3174..3851
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagaccg ccatgcgagc gcagtggccg ggacaactct
       61 gggccgctct gctggcgctg ggggcgctgg cgggcgttgt tgttggagag tccaacatct
      121 gtaccacacg aggcgtgaac tcctgccagc agtgtctggc tgtgagtcct gtgtgtgcct
      181 ggtgctcaga tgagactttg tctcagggct caccccgatg taacctgaag gagaacctgc
      241 tgaaggacaa ttgtgctcca gagtctattg agttcccagt cagtgaggcc cagatcctgg
      301 aggctaggcc actcagcagc aagggctctg gaagcagcgc ccagatcact caagtcagcc
      361 ctcagaggat tgcccttcga ctacggccag atgattcgaa gatcttctca cttcaagtgc
      421 ggcaggtgga ggattacccc gtggacatct actacttgat ggacctgtct ttctccatga
      481 aggatgatct gtccagcatc cagaccctgg gtaccaagtt ggcctctcag atgcgcaagc
      541 ttactagcaa ccttcggatc ggctttgggg ccttcgtgga caagcctgta tcgccgtaca
      601 tgtacatctc cccaccacag gcaatcaaaa acccctgtta caatatgaag aatgcctgct
      661 tgcccatgtt tggctacaaa cacgtgctga cgctaaccga ccaggtgtcc cgcttcaatg
      721 aagaagtgaa gaaacagagc gtgtcccgta atcgagatgc cccagagggc ggctttgacg
      781 ccatcatgca ggctacagta tgtgatgaaa aaattggctg gaggaatgac gcatcccatt
      841 tgctagtgtt taccacggat gccaagaccc atattgccct ggatggaagg ctggcaggca
      901 ttgtcctgcc caatgatggg cactgtcaca ttggcaccga caaccactac tctgcctcca
      961 ctaccatgga ctacccatct ctggggctga tgactgagaa actatcccag aaaaacatta
     1021 acttgatctt tgcagtgact gaaaatgtcg tcagccttta ccagaattat agtgagctca
     1081 ttcctgggac cacagtggga gtcctgtctg atgactcaag caacgtcctc cagctcattg
     1141 ttgatgctta cgggaaaatc cgctctaaag tggagctgga agtacgtgac ctgccggaag
     1201 aactgtcact gtccttcaat gccacctgcc tcaacaacga ggttatcccg ggcctcaagt
     1261 cttgtgtggg cctcaagatt ggagacacgg tgagctttag tatcgaggcc aaggtgcgtg
     1321 gctgccccca ggagaaggag cagtctttca ctatcaagcc tgtgggcttt aaggacagcc
     1381 tcaccgtcca ggtgaccttc gactgtgact gtgcctgcca ggcctttgcc cagccttcca
     1441 gcccacgctg caacaatggg aacgggactt ttgagtgtgg ggtgtgccgc tgtgaccagg
     1501 gctggctggg gtccatgtgt gagtgctctg aggaggatta ccgaccctct cagcaggaag
     1561 agtgcagccc caaggagggc cagcccatct gcagccagcg gggagagtgc ctctgtggcc
     1621 agtgtgtctg ccatagcagc gacttcggca agatcactgg caagtactgt gagtgcgatg
     1681 acttctcctg cgtccgctac aaaggggaga tgtgttccgg ccatgggcaa tgtaactgtg
     1741 gggactgcgt gtgtgactcg gactggactg gctactactg caactgtact acacgcactg
     1801 acacctgcat gtccaccaat gggctgctgt gcagcggccg gggcaactgc gagtgcggca
     1861 gctgtgtgtg cgtccagcca ggctcctatg gagacacctg tgagaagtgc cccacctgcc
     1921 cagatgcctg ctcctttaag aaggagtgtg tggagtgtaa gaagttcaac cggggaacgc
     1981 tccatgaaga aaacacctgc agccgctact gccgggacga catcgagcag gtgaaagagc
     2041 tgacggatac tggcaaaaac gccgtgaatt gtacctacaa gaacgaggat gactgtgtcg
     2101 tcagattcca gtactacgaa gacaccagtg ggagggcagt cctctatgtg gtggaagagc
     2161 ctgagtgtcc caagggtcct gatatcctgg tggtcctgct gtcagtgatg ggggccatcc
     2221 tgctcattgg ccttgctact ctgctcatct ggaagctact catcaccatc catgaccgga
     2281 aggaatttgc taaatttgag gaagaacgag ccagagccaa gtgggacaca gcaaacaacc
     2341 cgctgtataa agaggccacc tccaccttca ccaatatcac ctaccggggg actggtagcg
     2401 catccggggg tgcgtcaggc ggcagcggat ctggaccggt cgccaccatg agtgcgatta
     2461 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
     2521 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
     2581 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
     2641 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     2701 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
     2761 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
     2821 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     2881 aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag
     2941 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     3001 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
     3061 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     3121 gacgatccgg tagcggtcac ggtactggtt ctactggttc tggttcttct gagagtgcga
     3181 ttaagccaga catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg
     3241 tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag
     3301 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
     3361 gcaacagggt attcgccgaa tatccagacc acatacaaga ctattttaag cagtcgtttc
     3421 ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca
     3481 gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt cacggtgtaa
     3541 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg
     3601 agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct ttgttgcttg
     3661 aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg
     3721 tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag
     3781 attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg
     3841 ccagacgata agcggccgcg actctagatc ataatcagcc ataccacatt
//
