LOCUS       sequence-88785-e        3250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..3250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..713
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             762..1436
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatgag tgcgattaag ccagacatga
       61 agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg
      121 gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac
      181 ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg
      241 ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt
      301 gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa
      361 tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg
      421 gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc
      481 gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt
      541 accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct
      601 accactttgt ggaccactgc attgagattt taagccatga caaagattac aacaaggtta
      661 agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggta
      721 gcggtcacgg tactggttct actggttctg gttcttctga gagtgcgatt aagccagaca
      781 tgaagatcaa cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag
      841 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg
      901 gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat
      961 tcgccgaata tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt
     1021 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa
     1081 caatggaagg ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca
     1141 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg
     1201 tgcgtgatgg agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc
     1261 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
     1321 gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
     1381 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg
     1441 gactcagatc tcgagctcaa gcttcgatgg cgactgcgac ccccgtgccg ccgcggatgg
     1501 gcagccgcgc tggcggcccc accacgccgc tgagccccac gcgcctgtcg cggctccagg
     1561 agaaggagga gctgcgcgag ctcaatgacc ggctggcggt gtacatcgac aaggtgcgca
     1621 gcctggagac ggagaacagc gcgctgcagc tgcaggtgac ggagcgcgag gaggtgcgcg
     1681 gccgtgagct caccggcctc aaggcgctct acgagaccga gctggccgac gcgcgacgcg
     1741 cgctcgacga cacggcccgc gagcgcgcca agctgcagat cgagctgggc aagtgcaagg
     1801 cggaacacga ccagctgctc ctcaactatg ctaagaagga atctgatctt aatggcgccc
     1861 agatcaagct tcgagaatat gaagcagcac tcaattcgaa agatgcagct cttgctactg
     1921 cacttggtga caaaaaaagt ttagagggag atttggagga tctgaaggat cagattgccc
     1981 agttggaagc ctccttagct gcagccaaaa aacagttagc agatgaaact ttacttaaag
     2041 tagatttgga gaatcgttgt cagagcctta ctgaggactt ggagtttcgc aaaagcatgt
     2101 atgaagagga gattaacgag accagaagga agcatgaaac gcgcttggta gaggtggatt
     2161 ctgggcgtca aattgagtat gagtacaagc tggcgcaagc ccttcatgag atgagagagc
     2221 aacatgatgc ccaagtgagg ctgtataagg aggagctgga gcagacttac catgccaaac
     2281 ttgagaatgc cagactgtca tcagagatga atacttctac tgtcaacagt gccagggaag
     2341 aactgatgga aagccgcatg agaattgaga gcctttcatc ccagctttct aatctacaga
     2401 aagagtctag agcatgtttg gaaaggattc aagaattaga ggacttgctt gctaaagaaa
     2461 aagacaactc tcgtcgcatg ctgacagaca aagagagaga gatggcggaa ataagggatc
     2521 aaatgcagca acagctgaat gactatgaac agcttcttga tgtaaagtta gccctggaca
     2581 tggaaatcag tgcttacagg aaactcttag aaggcgaaga agagaggttg aagctgtctc
     2641 caagcccttc ttcccgtgtg acagtatccc gagcatcctc aagtcgtagt gtacgtacaa
     2701 ctagaggaaa gcggaagagg gttgatgtgg aagaatcaga ggcgagtagt agtgttagca
     2761 tctctcattc cgcctcagcc actggaaatg tttgcatcga agaaattgat gttgatggga
     2821 aatttatccg cttgaagaac acttctgaac aggatcaacc aatgggaggc tgggagatga
     2881 tcagaaaaat tggagacaca tcagtcagtt ataaatatac ctcaagatat gtgctgaagg
     2941 caggccagac tgttacaatt tgggctgcaa acgctggtgt cacagccagc cccccaactg
     3001 acctcatctg gaagaaccag aactcgtggg gcactggcga agatgtgaag gttatattga
     3061 aaaattctca gggagaggag gttgctcaaa gaagtacagt ctttaaaaca accatacctg
     3121 aagaagagga ggaggaggaa gaagcagctg gagtggttgt tgaggaagaa cttttccacc
     3181 agcagggaac cccaagagca tccaatagaa gctgtgcaat tatgtaggga tccaccggat
     3241 ctagataact
//
