LOCUS       sequence-88787-e        2763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2763)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..103,104..1261)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1322..1999
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2048..2725
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atggcggccc cgggcgcccg
       61 gcgtccgctg ctcctgttgc tgctggcagg ccttgcacac agcgccccag cactgttcga
      121 ggtgaaagac aacaacggca cagcgtgtat aatggccagc ttctctgcct cctttctgac
      181 cacctatgag gctggacatg tttctaaggt ctcgaatatg accctgccag cctctgcaga
      241 agtcctgaag aatagcagct cttgtggtga aaagaatgct tctgagccca ccctcgcaat
      301 cacctttgga gaaggatatt tactgaaact caccttcaca aaaaacacaa cacgttacag
      361 tgtccagcac atgtatttca catataacct gtcagacaca caattctttc ccaatgccag
      421 ctccaaaggg cccgacactg tggattccac aactgacatc aaggcagaca tcaacaaaac
      481 ataccgatgt gtcagcgaca tcagggtcta catgaagaat gtgaccattg tgctctggga
      541 cgctactatc caggcctacc tgccgagtag caacttcagc aaggaagaga cacgctgccc
      601 acaggatcaa ccttccccaa ctactgggcc acccagcccc tcaccaccac ttgtgcccac
      661 aaaccccagt gtgtccaagt acaatgtgac tggtgacaat ggaacctgcc tgctggcctc
      721 tatggcactg caactcaaca tcacctacat gaagaaggac aacacgactg tgaccagagc
      781 attcaacatc aacccaagtg acaaatatag tgggacttgc ggtgcccagt tggtgaccct
      841 gaaggtgggg aacaagagca gagtcctgga gctgcagttt gggatgaatg ccacttctag
      901 cctgtttttc ctgcaaggag ttcagttgaa catgactctt cctgatgcca tagagcccac
      961 gttcagcacc tccaactatt ccctgaaagc tcttcaggcc agtgtcggca actcatacaa
     1021 gtgcaacagt gaggagcaca tctttgtcag caaggcgctc gccctcaatg tcttcagcgt
     1081 gcaagtccag gctttcaggg tagaaagtga caggtttggg tctgtggaag agtgtgtaca
     1141 ggacggtaac aacatgctga tccccattgc tgtgggcggg gccctggcag ggctggtcct
     1201 catcgtcctc atcgcctacc tcatcggcag gaagaggagt cacgcgggct atcagaccat
     1261 ctcggaattc ggctccaccg gctccaccgg ctccaccggc gcggatccac cggtcgccac
     1321 catgagtgcg attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg
     1381 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
     1441 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
     1501 attccattac ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa
     1561 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
     1621 ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
     1681 tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
     1741 gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc
     1801 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
     1861 ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag
     1921 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     1981 gcctgacaat gccagacgat ccggtagcgg tcacggtact ggttctactg gttctggttc
     2041 ttctgagagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa
     2101 cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag
     2161 tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac
     2221 tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac aagactattt
     2281 taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg
     2341 catttgcatt gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg
     2401 atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg
     2461 ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg atattcgcat
     2521 ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc
     2581 taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt
     2641 aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg
     2701 attgcctgac aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca
     2761 cat
//
