LOCUS       sequence-88790-e        2576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2576)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..2576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1132..1809
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1858..2535
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 cgttcagatc cgctagcgct accggactca gatctcgagg ccaccatggc tgagccccgc
       61 caggagttcg aagtgatgga agatcacgct gggacgtacg ggttggggga caggaaagat
      121 caggggggct acaccatgca ccaagaccaa gagggtgaca cggacgctgg cctgaaagct
      181 gaagaagcag gcattggaga cacccccagc ctggaagacg aagctgctgg tcacgtgacc
      241 caagctcgca tggtcagtaa aagcaaagac gggactggaa gcgatgacaa aaaagccaag
      301 ggggctgatg gtaaaacgaa gatcgccaca ccgcggggag cagcccctcc aggccagaag
      361 ggccaggcca acgccaccag gattccagca aaaaccccgc ccgctccaaa gacaccaccc
      421 agctctggtg aacctccaaa atcaggggat cgcagcggct acagcagccc cggctcccca
      481 ggcactcccg gcagccgctc ccgcaccccg tcccttccaa ccccacccac ccgggagccc
      541 aagaaggtgg cagtggtccg tactccaccc aagtcgccgt cttccgccaa gagccgcctg
      601 cagacagccc ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact
      661 gagaacctga agcaccagcc gggaggcggg aaggtgcaaa tagtctacaa accagttgac
      721 ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca tccatcataa accaggaggt
      781 ggccaggtgg aagtaaaatc tgagaagctt gacttcaagg acagagtcca gtcgaagatt
      841 gggtccctgg acaatatcac ccacgtccct ggcggaggaa ataaaaagat tgaaacccac
      901 aagctgacct tccgcgagaa cgccaaagcc aagacagacc acggggcgga gatcgtgtac
      961 aagtcgccag tggtgtctgg ggacacgtct ccacggcatc tcagcaatgt ctcctccacc
     1021 ggcagcatcg acatggtaga ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc
     1081 tccctggcca agcagggttt gagctccgga ggggatccac cggtcgccac catgagtgcg
     1141 attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt
     1201 gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa
     1261 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
     1321 ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa gcagtcgttt
     1381 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc
     1441 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt tcacggtgta
     1501 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
     1561 gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc tttgttgctt
     1621 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
     1681 gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa
     1741 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
     1801 gccagacgat ccggtagcgg tcacggtact ggttctactg gttctggttc ttctgagagt
     1861 gcgattaagc cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac
     1921 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
     1981 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
     2041 tacggcaaca gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg
     2101 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
     2161 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg atttcacggt
     2221 gtaaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
     2281 actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg
     2341 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
     2401 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
     2461 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
     2521 aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca catttg
//
