LOCUS       sequence-88791-e        2551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2551)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..2551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1114..1791
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1840..2517
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggat ctgcaagcct cgttgctgtc cactggcccc
       61 aatgccagca acatctccga tggccaggat aatttcacat tggcggggcc acctcctcgc
      121 acaaggagtg tctcctacat caacatcatc atgccttcag tgtttggtac catctgtctc
      181 ctgggcattg tgggaaactc cacagtcatt tttgccgtgg tgaagaaatc caagctgcac
      241 tggtgcagca acgtccctga catcttcatc atcaacctct ctgtggtgga tctgcttttc
      301 ctgctgggca tgcctttcat gatccaccag ctcatgggta atggtgtctg gcactttggg
      361 gaaaccatgt gcaccctcat cacagccatg gacgccaaca gtcagttcac cagcacctac
      421 atcctgactg ctatggccat tgaccgctac ttggccaccg tccatcccat ctcctccacc
      481 aagttccgga agccctccat ggccaccctg gtgatctgcc tcctgtgggc tctctcgttc
      541 attagcatca ctcctgtgtg gctctatgcc aggcttatcc ccttcccagg gggtgctgtg
      601 ggctgtggca tccgcctacc aaacccagat actgatcttt actggttcac tctgtatcag
      661 tttttcctgg ccttcgccct tccgtttgtg gtcatcactg ctgcgtacgt gaaaatacta
      721 cagcgcatga cgtcttcggt ggccccagcc tctcaacgca gcatccggct tcggacaaag
      781 agggtgaccc gcacagccat tgccatctgt ctggtcttct ttgtgtgctg ggcgccctac
      841 tacgtgctgc agctgaccca gttgtccatc agccgcccga ccctcacatt cgtctacctg
      901 tacaatgcgg ctatcagctt gggctatgcc aacagctgcc tcaatccctt tgtgtacata
      961 gtactctgtg agacctttcg aaaacgcttg gtgctgtcgg tgaagcccgc ggcccagggg
     1021 cagcttcgca cggtcagcaa tgctcagaca gctgacgagg agaggacaga aagcaaaggc
     1081 accggaggta gcggggatcc accggtcgcc accatgagtg cgattaagcc agacatgaag
     1141 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
     1201 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
     1261 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
     1321 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
     1381 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
     1441 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
     1501 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
     1561 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
     1621 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
     1681 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
     1741 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
     1801 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
     1861 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
     1921 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
     1981 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
     2041 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     2101 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     2161 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     2221 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     2281 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     2341 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     2401 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     2461 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg
     2521 gccgcgactc ttagatcata atcagccata c
//
