LOCUS       sequence-88797-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-Mito-7
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..96
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             130..807
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             856..1533
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 ggacgggtca gatccgctag catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca
       61 ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg ggatccaccg
      121 gtcgccacca tgagtgcgat taagccagac atgaagatca acctccgtat ggaaggcaac
      181 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa
      241 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
      301 accactgcat tccattacgg caacagggta ttcgccgaat atccagacca catacaagac
      361 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
      421 gggggcattt gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa
      481 gttcgatttc acggtgtaaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
      541 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac tggtgatatt
      601 cgcatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
      661 aaagctaagg agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag
      721 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
      781 tctggattgc ctgacaatgc cagacgatcc ggtagcggtc acggtactgg ttctactggt
      841 tctggttctt ctgagagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
      901 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      961 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
     1021 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
     1081 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
     1141 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
     1201 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
     1261 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1321 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1381 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1441 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1501 cattctggat tgcctgacaa tgccagacga taagcggccg cgactctaga tcataatcag
     1561 cc
//
