LOCUS       sequence-88798-e        7444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7444)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..7444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             761..1435
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 accgctcgag ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
      241 cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggtag
      721 cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta agccagacat
      781 gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      841 tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg
      901 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      961 cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc
     1021 gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac
     1081 aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa
     1141 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
     1201 gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca
     1261 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
     1321 ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt
     1381 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
     1441 actcagatct ggcagcggtg gaggcagcgc atccggcgga agcggaagcg ctcagcaggc
     1501 tgcagacaag tacctctatg tggataaaaa cttcatcaat aacccgctgg cccaagctga
     1561 ctgggctgcc aagaagttgg tatgggtgcc ttccagcaag aatggctttg aaccagccag
     1621 cctcaaggag gaggtgggag aagaggccat tgtagagctg gtagagaatg ggaagaaggt
     1681 gaaggtgaac aaggacgaca tccagaagat gaacccaccc aagttctcca aggtggagga
     1741 catggcagag ctcacgtgcc tcaacgaagc ttcggtgctg cacaacctca aggagcgata
     1801 ctactcaggg cttatctaca cctattcagg cctgttctgt gtggtcatca acccttataa
     1861 gaacctgccc atctactcag aggagatcgt ggagatgtac aagggcaaga agaggcacga
     1921 gatgccaccc cacatctacg ccatcacaga tactgcctac cggagcatga tgcaggaccg
     1981 ggaagatcag tccatcctgt gcacggggga gtctggagca gggaagacag agaacaccaa
     2041 gaaagtcatc cagtacctgg cacatgtggc ctcctcacac aagagcaaga aggaccaggg
     2101 ggagttggag cggcagctgc tacaggccaa ccctatccta gaggcctttg gaaacgccaa
     2161 gacggtgaag aatgacaact cctctcgatt cggtaaattc attcgtatca actttgatgt
     2221 caatggctac attgttggtg ccaacattga gacttatctt ctggagaaat ctcgtgctat
     2281 ccgccaagcc aaagaggagc ggaccttcca catcttctac tacctgctgt ctggggccgg
     2341 agaacacctg aagactgatc tcctgttgga gccatacaac aaataccgct tcctgtccaa
     2401 cgggcacgtc accatccctg ggcagcagga caaggacatg ttccaggaga caatggaggc
     2461 catgagaatt atgggtatcc cagaggatga gcagatgggc ttgctgcggg tcatctctgg
     2521 ggtccttcag cttggcaaca ttgccttcaa gaaggagcgg aacactgacc aggcgtccat
     2581 gccggacaac acagctgctc aaaaggtgtc ccacctcctg gggatcaatg tgaccgactt
     2641 caccagaggc atcctcaccc cacgcatcaa ggtgggcaga gactatgtgc agaaggcgca
     2701 gactaaagag caggctgact ttgccattga ggccttggcc aaggctacct atgagcggat
     2761 gttccgctgg ctggtgcttc gcatcaacaa agctctggac aagaccaaga ggcagggcgc
     2821 ctcatttatc gggatactgg acatcgctgg ctttgaaatc tttgatctga actccttcga
     2881 gcagctgtgc atcaactaca ccaacgagaa gctgcagcag ctgttcaacc acaccatgtt
     2941 catcctggag caggaggagt accagcgaga gggcatcgag tggaacttca tcgacttcgg
     3001 cctggacctg cagccctgca tcgacctcat tgagaagccg gcgggtcccc caggcatcct
     3061 ggccctgcta gatgaggagt gctggtttcc taaggccact gacaagagct tcgtggagaa
     3121 ggtggtgcag gagcagggca cccaccccaa gttccagaag cccaagcaac tgaaggacaa
     3181 ggctgatttc tgcattatcc actatgccgg caaggtggac tataaagctg acgagtggct
     3241 gatgaagaac atggacccct tgaacgacaa catcgccacg ctgcttcacc agtcctcaga
     3301 caagtttgtc tctgagctgt ggaaggatgt ggatcggatc attggcttgg accaagtggc
     3361 tggaatgtcc gagacagcac tacctggtgc cttcaagacc cggaagggca tgttccgtac
     3421 tgtcggacag ctgtacaagg agcagctggc caagctcatg gccacgttga ggaataccaa
     3481 ccccaacttc gtgcgctgca tcattcccaa ccatgagaag aaggccggca aactggaccc
     3541 gcacttggtg ctggaccagc tgcgctgcaa tggcgtcctt gagggcatcc ggatctgccg
     3601 ccagggcttt cccaacaggg tggtcttcca ggagttccgg cagaggtatg agatcctcac
     3661 ccccaactcc atcccgaagg gcttcatgga tggcaagcaa gcgtgtgtgc tcatgatcaa
     3721 agccttggag cttgacagca acctgtaccg catcggccag agcaaagtgt tcttccgggc
     3781 aggagtgctc gcccacctgg aggaagagcg ggacctgaag atcaccgatg tcatcattgg
     3841 cttccaggcc tgctgcaggg gctacctggc caggaaggcc tttgccaaga ggcagcaaca
     3901 gctgaccgcc atgaaggtcc tacagaggaa ctgtgctgcg tacctcaggc tgcgcaactg
     3961 gcagtggtgg aggctcttca ccaaggtcaa gcccctgttg aactcaataa gacatgagga
     4021 tgagctgtta gccaaggagg cggaactgac aaaggttcga gagaaacatc tggctgcaga
     4081 gaacaggctg acagagatgg agacgatgca gtctcagctc atggcagaga agctgcagct
     4141 tcaggagcag ctgcaggcgg agacagagct gtgtgccgag gctgaggagc tccgggcccg
     4201 tctgacagcg aagaagcagg agctggagga gatatgccat gacctggagg ccagggtgga
     4261 ggaggaggag gagcgctgcc agtacctgca ggccgagaag aagaagatgc agcagaacat
     4321 ccaggaactt gaggagcagt tggaggagga ggagagcgcc cggcagaagc tgcagcttga
     4381 gaaggtgacc accgaggcca agctgaagaa actggaggag gaccagatca tcatggagga
     4441 ccagaactgc aaactggcca aggagaagaa actgctggaa gacagagtag ctgagttcac
     4501 taccaacctc atggaagagg aggagaagtc caagagcctg gccaagctca agaacaagca
     4561 cgaggcaatg atcaccgacc tggaagagcg cctccgtagg gaggagaagc agaggcagga
     4621 gttggagaag acccgtcgca agctggaggg agactccaca gacctcagtg accagattgc
     4681 tgagctccag gcgcagatag cagagctcaa gatgcagctg gccaagaagg aggaggagtt
     4741 gcaggctgcc ttggccagag tggaagaaga agctgctcag aagaatatgg ccctgaagaa
     4801 gatccgagaa ctggaaactc agatatctga gctccaggag gacctggagt cggagcgagc
     4861 ctccaggaat aaagccgaga agcagaaacg ggatctggga gaggagctgg aggcgctgaa
     4921 gacagagctg gaggacacgc tggactccac ggctgcccag caggagctga ggtcgaagcg
     4981 tgagcaggag gtgagcatcc tgaagaagac tctggaggac gaggccaaga cccatgaggc
     5041 ccagatccag gagatgaggc agaagcactc acaggctgtg gaggagctgg cagatcagtt
     5101 ggagcagacg aagcgggtaa aagctaccct tgagaaggcg aagcagaccc tggagaatga
     5161 gcggggagag ctggccaatg aggtgaaggc cctgctgcaa ggcaagggcg actcagagca
     5221 caagcgcaag aaggtggagg cgcagctgca agaactgcag gtcaagttca gcgagggaga
     5281 gcgcgtgcga accgaactgg ccgacaaggt caccaagctg caggttgaac tggacagtgt
     5341 gaccggtctc cttagccagt ctgacagcaa gtccagcaag cttacgaagg acttctctgc
     5401 gctggagtcc cagcttcagg acacacagga gttgctccag gaggagaacc ggcagaagct
     5461 gagcctgagc accaagctca agcagatgga ggatgagaaa aactccttca gggagcagct
     5521 ggaggaggag gaggaggcca agcgcaactt ggagaagcag atcgccacgc tccatgccca
     5581 ggtgaccgac atgaagaaga agatggagga cggtgtaggg tgcctggaga ctgcagagga
     5641 ggcgaagcgg aggcttcaga aggacttgga aggcctgagc cagcggcttg aggagaaggt
     5701 ggctgcctac gataagctgg agaagaccaa gacacggctg cagcaggagc tggacgacct
     5761 gctggttgac ctggaccacc agcggcagag cgtctccaac ctggaaaaga agcagaagaa
     5821 gttcgaccag ctcctagccg aggagaagac catctcggcc aagtatgcag aggagcgtga
     5881 ccgagctgag gctgaggccc gtgagaagga gacaaaggcg ctatcactgg cccgggcgct
     5941 tgaggaggcc atggagcaga aggcagagct ggagcggctc aacaagcagt tccgcacgga
     6001 gatggaggac ctcatgagct ccaaggatga cgtgggcaag agtgtccacg agctggagaa
     6061 gtccaagcgg gccttggagc agcaggtgga ggagatgaag acccagctgg aggagctgga
     6121 ggatgagctg caggccacgg aggatgccaa gctccgcctg gaggtgaacc tgcaggccat
     6181 gaaggcccag tttgagcggg atctgcaggg ccgggatgaa cagagcgagg agaagaagaa
     6241 gcagctggtc agacaggtgc gggagatgga ggcggagctg gaggatgaga ggaagcagcg
     6301 ctccatggcc atggccgcac gcaagaaact ggagatggat ctgaaggacc tggaggcaca
     6361 cattgacaca gccaataaga accgggaaga ggccatcaaa cagctgcgga agcttcaggc
     6421 ccagatgaag gactgcatgc gggagctgga cgacacgcgc gcctcccggg aggagatcct
     6481 ggcgcaggcc aaggagaatg agaagaagct gaagagcatg gaggccgaga tgattcagct
     6541 gcaggaggaa ctggcagctg ctgagcgtgc taagcgtcag gcccaacagg aacgggacga
     6601 gctggctgat gagatcgcca acagcagtgg caaaggggcc ctagcattag aggagaagcg
     6661 gcgactggag gcccgcattg ccctgctgga ggaggagctg gaggaggaac agggcaacac
     6721 ggagctgatc aacgatcggc tgaagaaggc caacctgcag atcgaccaaa taaacaccga
     6781 cctgaacctg gaacgcagcc acgcacagaa gaatgagaat gcgcgacagc agctggaacg
     6841 ccagaacaag gagctcaagg ccaagctgca ggaaatggag agtgctgtca agtccaaata
     6901 caaggcctcc atcgcggcct tggaggccaa aattgcacag ctggaggaac agctggacaa
     6961 cgagaccaag gagcgccagg cagcctccaa gcaggtgcgc cggacggaga agaagctgaa
     7021 ggacgtgctg ctgcaggtgg aggacgagcg gaggaacgcg gaacagttca aggaccaggc
     7081 tgacaaggcg tccacccgcc tgaagcagct taaacggcag ctagaggagg ctgaagagga
     7141 ggcccagcgg gccaatgcct cacgccggaa gctgcagcgt gagctggaag atgccacaga
     7201 gaccgctgat gctatgaacc gcgaggtcag ctccctgaag aacaaactga ggcgtgggga
     7261 cctgccattt gtcgtgactc gccgaattgt tcggaaaggc actggcgact gctcagacga
     7321 ggaggtcgac ggtaaagcag atggggccga tgccaaggca gctgaatagg aattctgcag
     7381 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
     7441 ccac
//
