LOCUS       sequence-88801-e        7527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7527)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..7527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6088..6765
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             6814..7491
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 ttagttgacg tcagatccgc tagcaccgcc accatggctg cagtgaccat gtccgtgtct
       61 gggaggaagg tagcctccag gccaggcccg gtgcctgagg cagcccaatc gttcctctac
      121 gcgccccgga cgccaaatgt aggtggccct ggagggccac aggtggagtg gacagcccgg
      181 cgcatggtgt gggtgccctc ggaactgcat gggttcgagg cagcagccct gcgggatgaa
      241 ggggaggagg aggcagaagt ggagctggcg gagagtgggc gccgcctgcg gctgcccagg
      301 gaccagatcc agcgcatgaa cccacccaag ttcagcaagg cagaagatat ggctgagctc
      361 acctgcctca acgaggcctc ggtcctgcac aacctgcgag aacgctacta ctccgggctc
      421 atttatacct actctggcct cttctgtgtg gtcattaacc catacaagca gctgcccatc
      481 tacacggagg ccattgttga aatgtaccgg ggcaagaagc gccatgaggt gccacctcac
      541 gtgtatgctg tgacggaggg cgcgtaccgc agcatgcttc aggatcgtga ggatcaatcc
      601 attctctgca cgggagagtc tggcgctggg aagacggaga acaccaagaa ggtcatccag
      661 tacctggccc atgtggcatc atctccaaag ggcaggaagg agcctggtgt ccctgcctcc
      721 gtcagcacca tgtcttatgg ggagctagag cgtcagcttc ttcaagccaa ccccatccta
      781 gaggcctttg gcaatgccaa gacagtgaag aacgacaact cttcccgatt tggcaaattc
      841 atccgcatca actttgatat tgctggctac atcgtgggag caaacatcga gacctatctg
      901 ttggagaagt cccgggccat cagacaggcc aaggatgaat gcagcttcca tatcttctac
      961 cagctgctag ggggcgctgg ggagcagcta aaagctgacc tccttctgga gccctgttcc
     1021 cattatcgct tcctgaccaa tgggccctca tcgtccccgg gccaggagcg tgagttattc
     1081 caggagaccc tggagtccct gcgtgtgctg ggcctcctcc cagaagagat cactgccatg
     1141 ctgcgcactg tctctgctgt cctccagttt ggcaacattg tcctgaagaa agagcgcaat
     1201 acggaccaag ccaccatgcc tgacaacaca gctgcccaga agctttgccg cctcttggga
     1261 ctcggagtga ccgacttctc cagagccctt ctcacacccc gcatcaaagt gggccgagat
     1321 tatgttcaga aagcacaaac caaggagcag gctgactttg cgctggaggc tctggccaaa
     1381 gctacctatg agcgcctgtt ccgctggctg gttctgcggc tcaaccgtgc cctggacaga
     1441 agcccgcggc agggtgcctc cttcctgggc atcctggaca tcgcgggctt tgagatcttc
     1501 cagctgaact ccttcgagca gctgtgcatc aactacacca acgagaagct acagcagcta
     1561 ttcaaccaca ccatgttcgt gctggagcag gaggagtacc agcgagaggg catcccctgg
     1621 accttcctag acttcgggtt ggacctgcaa ccttgcatcg acctcattga gcgtccggcc
     1681 aaccctccag gtctcctggc cctgctggac gaggagtgct ggttccccaa ggccacggac
     1741 aagtcttttg tggagaaggt cgcccaggag cagggcagcc accccaaatt ccagcgcccc
     1801 aggaacctgc gagatcaggc cgacttcagc gtcctgcact acgccggcaa ggttgactac
     1861 aaagccagtg agtggctgat gaagaacatg gacccactga atgacaatgt ggccgccttg
     1921 cttcaccaga gcacggatcg tctcacagct gagatctgga aggatgtgga gggcatcgtg
     1981 gggctggagc aagtaagcag ccttggagat ggcccaccgg gaggccgccc ccgccgtgga
     2041 atgtttcgga ctgtggggca gctctacaaa gaatccctga gccgcctcat ggccacgctc
     2101 agcaacacca accctagttt tgtccgctgc atcgttccca atcatgagaa gagggctgga
     2161 aagctggagc cgcgcctggt gctggaccaa ctccgttgta acggggtcct cgagggtata
     2221 cgcatctgtc gccaaggctt ccccaaccgc atcctcttcc aggagttccg acagcgctat
     2281 gaaatcctca ccccgaacgc tattcccaag ggcttcatgg acggcaaaca ggcctgtgag
     2341 aagatgatcc aggccctgga gctagacccc aacctgtacc gtgttggcca aagcaagatc
     2401 ttcttccggg caggggtcct ggcccagctg gaggaggagc gggacctgaa agtcaccgac
     2461 atcatagtgt ctttccaggc agcggcacgg ggctacctgg cccgtagggc tttccagaga
     2521 cggcagcagc agcagagtgc tctgagggtg atgcagagaa actgtgctgc ctacctcaag
     2581 ctcaggaact ggcagtggtg gaggctgttc atcaaggtga agcccctgct gcaggtgaca
     2641 cggcaggatg aggtgctgca ggcgcgcgcc caggagctgc agaaagttca ggagctgcag
     2701 cagcagagcg ctcgtgaagt gggggaactg cagggtcgag tggcacagct agaggaggag
     2761 cgcacgcgcc tggctgagca gcttcgagca gaagccgagc tctgctctga ggccgaggag
     2821 acgcgggcgc gactggctgc ccggaagcag gagctggagc tggtggtgac agagctggag
     2881 gcacgagtgg gcgaggaaga agagtgcagc cggcagctgc agagtgagaa gaagaggctg
     2941 cagcagcata tccaggagct agagagccac ctggaagctg aggagggtgc ccggcagaag
     3001 ctacagctgg agaaggtgac cacagaggcc aagatgaaga aatttgagga ggacctgctg
     3061 ctcctggagg accagaattc caagctgagc aaggagcgga ggctgctgga ggagcggctg
     3121 gctgagttct cctcacaggc agcagaagag gaagagaaag tcaaaagtct caacaagctg
     3181 aggctcaaat atgaagccac aatctcagac atggaagacc ggctgaagaa ggaggagaag
     3241 ggacgccagg aactagagaa gctgaagcga cggctggacg gggagagctc agagcttcag
     3301 gagcagatgg tggagcagaa gcagagggca gaggaactgc tcgcacagct gggccgcaag
     3361 gaggatgagc tgcaggccgc cctgctcagg gcagaggaag agggtggtgc ccgtgcccag
     3421 ttgctcaagt ccctgcgaga ggcacaggct ggccttgctg aggctcagga ggacctggaa
     3481 gctgagcggg tagccagggc caaggcggag aagcagcgcc gggacctggg cgaggagttg
     3541 gaggccctac gtggggagct cgaggacact ctggattcca ccaacgccca gcaggagctg
     3601 cggtccaaga gggagcagga ggtgacagag ctgaagaaag cattggaaga ggagtcccgt
     3661 gcccatgagg tgtccatgca ggagctgaga cagaggcata gccaggcact ggtggagatg
     3721 gccgagcagt tggagcaagc ccggaggggc aaaggtgtgt gggagaagac tcggctatcc
     3781 ctggaggctg aggtgtccga gctgaaggcc gagctgagca gcctgcagac ctcgagacag
     3841 gagggtgagc agaagaggcg ccgcctggag tcccagctac aggaggtcca gggccgatcc
     3901 agtgattcgg agcgggctcg gtctgaggct gctgagaagc tgcagagagc ccaggcggaa
     3961 cttgagagcg tgtccacagc cctgagtgag gcggagtcca aagccatcag gctgggcaag
     4021 gagctgagca gtgcagagtc ccagctgcat gacacccagg aactgcttca ggaggagacc
     4081 agggcaaagc tggccttggg gtcccgtgtg cgtgccctag aggccgaggc ggcggggctt
     4141 cgggagcaga tggaagagga ggtggttgcc agggaacggg ctggccggga gctgcagagc
     4201 acgcaggccc agctctctga atggcggcgc cgccaggaag aagaggctgc ggtgctggag
     4261 gctggggagg aggctcggcg ccgtgcagcc cgggaggcag agaccctgac ccagcgcctg
     4321 gcagaaaaga ctgaggctgt agaacgactg gagcgagccc ggcgccgact gcagcaggag
     4381 ttggacgatg ccactgtgga tctggggcag cagaagcagc tcctgagcac actggagaag
     4441 aagcagcgga aatttgacca gctcctggca gaggagaagg ctgcagttct acgggctgtg
     4501 gaagaccgtg aacggataga ggccgaaggc cgggagcgag aggcccgggc cctgtcgctg
     4561 acccgggccc tggaagagga gcaggaggcc cgggaggagc tggagaggca gaaccgtgct
     4621 ctgagggctg agctggaagc actgctgagc agcaaggatg acgtgggcaa gaacgtgcac
     4681 gagctggagc gagcccgtaa ggcggctgaa caggcagcca gtgacctgcg gacacaggtg
     4741 acagaattgg aggatgagct gacagccgca gaggatgcca agctgcgcct ggaggtgact
     4801 gtgcaggctc tgaaggctca acatgaacgc gacctgcagg gccgcgatga tgccggtgag
     4861 gagaggcgga ggcagctggc caagcagcta agagacgcag aggtagagcg cgatgaggaa
     4921 cggaagcaga gggcactggc tatggctgcc cgcaagaagc tggagctgga actggaggag
     4981 ttgaaggcgc agacatctgc tgctgggcag ggcaaggaag aggcagtgaa gcagctgaag
     5041 aagatgcagg tccagatgaa ggagctgtgg cgggaggtag aggagacgcg tagctcccgc
     5101 gacgagatgt ttaccctgag cagggaaaat gagaagaagc tcaaggggct ggaagctgag
     5161 gtgctgcgtc tgcaagagga acttgctgcc tcagaccgag cccggaggca ggcccagcaa
     5221 gacagagacg agatggcaga ggaggtggcc agtggcaatc ttagcaaggc agccaccctg
     5281 gaggaaaaac ggcagctgga ggggcgactg agccagttgg aagaggagct ggaggaagaa
     5341 cagaacaact cggagctgct caaggaccat taccgaaagc tagtgctaca ggtcgagtcc
     5401 ctcaccacag aactgtctgc cgaacgaagt ttctcagcca aggccgagag tggacggcag
     5461 cagctggagc ggcagatcca ggaactgcgg gcccgcttgg gtgaagagga tgctggagcc
     5521 cgagccaggc agaaaatgct gatcgctgct ctggagtcta aactggccca ggcagaggag
     5581 cagctggagc aggagagcag ggagcgcatc ctctctggca agctggtacg cagagctgag
     5641 aagcggctga aggaggtagt tcttcaggtg gatgaagagc gcagggtggc tgaccaggtc
     5701 cgggaccagc tggagaaaag caacctccgg ctgaagcagc tcaagaggca gctggaggag
     5761 gcagaggagg aggcatctcg ggcacaggct ggtcggaggc ggctgcagcg ggagctggag
     5821 gacgtcactg agtctgcaga atccatgaac cgggaggtga ccacgctgag gaacaggctc
     5881 cggcgtggcc cacttacatt caccacacgg actgtgcgcc aggtgttccg gctggaagag
     5941 ggcgtggctt ctgacgagga agaggctgaa ggagctgaac ctggctctgc accaggccag
     6001 gagccggagg ctccgccccc tgccacaccc caaagcagcg gtggagcaag cgcagccagt
     6061 ggtagcgcgg atccaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac
     6121 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
     6181 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
     6241 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat
     6301 ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
     6361 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
     6421 gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt
     6481 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
     6541 gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt
     6601 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
     6661 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
     6721 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac
     6781 ggtactggtt ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc
     6841 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     6901 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     6961 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
     7021 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     7081 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     7141 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
     7201 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     7261 ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga
     7321 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     7381 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     7441 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg
     7501 actctagatc ataatcagcc ataccac
//
