LOCUS       sequence-88803-e        1704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1704)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..1704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..74
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     25..45
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             119..796
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             845..1519
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             1544..1564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1568..1588
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1592..1612
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
       61 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat
      121 gagtgcgatt aagccagaca tgaagatcaa cctccgtatg gaaggcaacg taaacgggca
      181 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
      241 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
      301 ccattacggc aacagggtat tcgccgaata tccagaccac atacaagact attttaagca
      361 gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg
      421 cattgccaga aacgacataa caatggaagg ggacactttc tataataaag ttcgatttca
      481 cggtgtaaac tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc
      541 ctccactgag aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc gcatggcttt
      601 gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga
      661 gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca
      721 tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc
      781 tgacaatgcc agacgatccg gtagcggtca cggtactggt tctactggtt ctggttcttc
      841 tgagagtgcg attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg
      901 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
      961 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
     1021 attccattac ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa
     1081 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
     1141 ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
     1201 tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
     1261 gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc
     1321 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
     1381 ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag
     1441 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     1501 gcctgacaat gccagacgat ccggactcag atctcgagct gatccaaaaa agaagagaaa
     1561 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccac
     1621 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1681 aaaaaacctc ccacacctcc ccct
//
