LOCUS       sequence-88804-e        3064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3064)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Occludin-N-10
FEATURES             Location/Qualifiers
     source          1..3064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..60
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1622..2299
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2348..3025
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgtc atccaggcct cttgaaagtc cacctcctta
       61 caggcctgat gaattcaaac cgaatcatta tgcaccaagc aatgacatat atggtggaga
      121 gatgcatgtt cgaccaatgc tctctcagcc agcctactct ttttacccag aagatgaaat
      181 tcttcacttc tacaaatgga cctctcctcc aggagtgatt cggatcctgt ctatgctcat
      241 tattgtgatg tgcattgcca tctttgcctg tgtggcctcc acgcttgcct gggacagagg
      301 ctatggaact tcccttttag gaggtagtgt aggctaccct tatggaggaa gtggctttgg
      361 tagctacgga agtggctatg gctatggcta tggttatggc tatggctacg gaggctatac
      421 agacccaaga gcagcaaagg gcttcatgtt ggccatggct gccttttgtt tcattgccgc
      481 gttggtgatc tttgttacca gtgttataag atctgaaatg tccagaacaa gaagatacta
      541 cttaagtgtg ataatagtga gtgctatcct gggcatcatg gtgtttattg ccacaattgt
      601 ctatataatg ggagtgaacc caactgctca gtcttctgga tctctatatg gttcacaaat
      661 atatgccctc tgcaaccaat tttatacacc tgcagctact ggactctacg tggatcagta
      721 tttgtatcac tactgtgttg tggatcccca ggaggccatt gccattgtac tggggttcat
      781 gattattgtg gcttttgctt taataatttt ctttgctgtg aaaactcgaa gaaagatgga
      841 caggtatgac aagtccaata ttttgtggga caaggaacac atttatgatg agcagccccc
      901 caatgtcgag gagtgggtta aaaatgtgtc tgcaggcaca caggacgtgc cttcaccccc
      961 atctgactat gtggaaagag ttgacagtcc catggcatac tcttccaatg gcaaagtgaa
     1021 tgacaagcgg ttttatccag agtcttccta taaatccacg ccggttcctg aagtggttca
     1081 ggagcttcca ttaacttcgc ctgtggatga cttcaggcag cctcgttaca gcagcggtgg
     1141 taactttgag acaccttcaa aaagagcacc tgcaaaggga agagcaggaa ggtcaaagag
     1201 aacagagcaa gatcactatg agacagacta cacaactggc ggcgagtcct gtgatgagct
     1261 ggaggaggac tggatcaggg aatatccacc tatcacttca gatcaacaaa gacaactgta
     1321 caagaggaat tttgacactg gcctacagga atacaagagc ttacaatcag aacttgatga
     1381 gatcaataaa gaactctccc gtttggataa agaattggat gactatagag aagaaagtga
     1441 agagtacatg gctgctgctg atgaatacaa tagactgaag caagtgaagg gatctgcaga
     1501 ttacaaaagt aagaagaatc attgcaagca gttaaagagc aaattgtcac acatcaagaa
     1561 gatggttgga gactatgata gacagaaaac aagcggctcc ggtagcggac cggtcgccac
     1621 catgagtgcg attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg
     1681 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
     1741 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
     1801 attccattac ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa
     1861 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
     1921 ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
     1981 tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
     2041 gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc
     2101 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
     2161 ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag
     2221 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     2281 gcctgacaat gccagacgat ccggtagcgg tcacggtact ggttctactg gttctggttc
     2341 ttctgagagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa
     2401 cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag
     2461 tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac
     2521 tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac aagactattt
     2581 taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg
     2641 catttgcatt gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg
     2701 atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg
     2761 ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg atattcgcat
     2821 ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc
     2881 taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt
     2941 aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg
     3001 attgcctgac aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca
     3061 catt
//
