LOCUS       sequence-88807-e        1699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: tdEos-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..1699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     CDS             255..932
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             981..1658
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatgagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag
      301 gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg
      361 gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata
      421 tcctgaccac tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac
      481 aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg
      541 aagacggggg catttgcatt gccagaaacg acataacaat ggaaggggac actttctata
      601 ataaagttcg atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga
      661 cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg
      721 atattcgcat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta
      781 cttacaaagc taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca
      841 ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg
      901 ctcattctgg attgcctgac aatgccagac gatccggtag cggtcacggt actggttcta
      961 ctggttctgg ttcttctgag agtgcgatta agccagacat gaagatcaac ctccgtatgg
     1021 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
     1081 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
     1141 atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat ccagaccaca
     1201 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
     1261 tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct
     1321 ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga
     1381 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgactg
     1441 gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
     1501 ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact
     1561 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     1621 ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata
     1681 atcagccata ccacattga
//
