LOCUS       sequence-88812-e        2095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, 
            Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, 
            Bewersdorf J
  TITLE     Video-rate nanoscopy using sCMOS camera-specific single-molecule 
            localization algorithms.
  JOURNAL   Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 
            2013 May 26.
  PUBMED    23708387
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2488"; journalName: "Nat Methods"; date: "2013-07"; 
            volume: "10"; issue: "7"; pages: "653-8"
COMMENT     Sequence Label: tdEos-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..2095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             652..1329
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1378..2055
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccggcta gcgctaccgg actcagatct gccaccatgg cgccggccgc gtccaggctg
       61 cgggccgaag ccgggctcgg ggcgctgccg cggcgggcgc tcgcccagta cttgctcttc
      121 ctgcggctct acccggtgct caccaaggcg gccaccagtg gcattttgtc agcacttggg
      181 aacttcctgg cccagatgat tgagaagaag cggaaaaaag aaaactctag aagtctggat
      241 gtcggtgggc ctctgagata tgccgtttac gggttcttct tcacagggcc gctgagtcac
      301 ttcttctact tcttcatgga acattggatc cctcctgagg tccccctggc agggctcagg
      361 aggcttctcc tggaccgcct cgtctttgca ccggccttcc tcatgttgtt cttcctcatc
      421 atgaactttc tggaggggaa agacgcctca gccttcgccg ccaagatgag ggggggcttc
      481 tggccggcgc tgaggatgaa ctggcgggtg tggacgccac tacagttcat caacatcaac
      541 tacgtccctc tgaagttccg ggtgctcttc gccaacctgg cagctctgtt ctggtatgcc
      601 tacctggcct ccttggggaa gagcggctcc ggtagcggac cggtcgccac catgagtgcg
      661 attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt
      721 gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa
      781 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
      841 ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa gcagtcgttt
      901 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc
      961 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt tcacggtgta
     1021 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
     1081 gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc tttgttgctt
     1141 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
     1201 gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa
     1261 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
     1321 gccagacgat ccggtagcgg tcacggtact ggttctactg gttctggttc ttctgagagt
     1381 gcgattaagc cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac
     1441 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
     1501 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
     1561 tacggcaaca gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg
     1621 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt
     1681 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg atttcacggt
     1741 gtaaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
     1801 actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg
     1861 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
     1921 ggtgtcaagt taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac
     1981 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
     2041 aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca cattt
//
