LOCUS       sequence-88814-e        4659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4659)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-PTK2-N-10
FEATURES             Location/Qualifiers
     source          1..4659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3217..3894
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3943..4620
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacagatccg ctagcgccac catggcagct gcttaccttg acccccactt gaatcacaca
       61 ccaaattcga gtactaagac tcacctgggt actggtatgg aacgttctcc tggtgcaatg
      121 gagcgagtat taaaggtctt tcattatttt gaaagcaata gtgagccaac cacctgggcc
      181 agtattatca ggcatggaga tgctactgat gtcaggggca tcattcagaa gatagtggac
      241 agtcacaaag taaagcatgt ggcctgctat ggattccgcc tcagtcacct gcggtcagag
      301 gaggttcact ggcttcacgt ggatatgggc gtctccagtg tgagggagaa gtatgagctt
      361 gctcacccac cagaggagtg gaaatatgaa ttgagaattc gttatttgcc aaaaggattt
      421 ctaaaccagt ttactgaaga taagccaact ttgaatttct tctatcaaca ggtgaagagc
      481 gattatatgt tagagatagc tgatcaagtg gaccaggaaa ttgctttgaa gttgggttgt
      541 ctagaaatac ggcgatcata ctgggagatg cggggcaatg cactagaaaa gaagtctaac
      601 tatgaagtat tagaaaaaga tgttggttta aagcgatttt ttcctaagag tttactggat
      661 tctgtcaagg ccaaaacact aagaaaactg atccaacaaa catttagaca atttgccaac
      721 cttaatagag aagaaagtat tctgaaattc tttgagatcc tgtctccagt ctacagattt
      781 gataaggaat gcttcaagtg tgctcttggt tcaagctgga ttatttcagt ggaactggca
      841 atcggcccag aagaaggaat cagttaccta acggacaagg gctgcaatcc cacacatctt
      901 gctgacttca ctcaagtgca aaccattcag tattcaaaca gtgaagacaa ggacagaaaa
      961 ggaatgctac aactaaaaat agcaggtgca cccgagcctc tgacagtgac ggcaccatcc
     1021 ctaaccattg cggagaatat ggctgaccta atagatgggt actgccggct ggtgaatgga
     1081 acctcgcagt catttatcat cagacctcag aaagaaggtg aacgggcttt gccatcaata
     1141 ccaaagttgg ccaacagcga aaagcaaggc atgcggacac acgccgtctc tgtgtcagaa
     1201 acagatgatt atgctgagat tatagatgaa gaagatactt acaccatgcc ctcaaccagg
     1261 gattatgaga ttcaaagaga aagaatagaa cttggacgat gtattggaga aggccaattt
     1321 ggagatgtac atcaaggcat ttatatgagt ccagagaatc cagctttggc ggttgcaatt
     1381 aaaacatgta aaaactgtac ttcggacagc gtgagagaga aatttcttca agaagcctta
     1441 acaatgcgtc agtttgacca tcctcatatt gtgaagctga ttggagtcat cacagagaat
     1501 cctgtctgga taatcatgga gctgtgcaca cttggagagc tgaggtcatt tttgcaagta
     1561 aggaaataca gtttggatct agcatctttg atcctgtatg cctatcagct tagtacagct
     1621 cttgcatatc tagagagcaa aagatttgta cacagggaca ttgctgctcg gaatgttctg
     1681 gtgtcctcaa atgattgtgt aaaattagga gactttggat tatcccgata tatggaagat
     1741 agtacttact acaaagcttc caaaggaaaa ttgcctatta aatggatggc tccagagtca
     1801 atcaattttc gacgttttac ctcagctagt gacgtatgga tgtttggtgt gtgtatgtgg
     1861 gagatactga tgcatggtgt gaagcctttt caaggagtga agaacaatga tgtaatcggt
     1921 cgaattgaaa atggggaaag attaccaatg cctccaaatt gtcctcctac cctctacagc
     1981 cttatgacga aatgctgggc ctatgacccc agcaggcggc ccaggtttac tgaacttaaa
     2041 gctcagctca gcacaatcct ggaggaagag aaggctcagc aagaagagcg catgaggatg
     2101 gagtccagaa gacaggccac agtgtcctgg gactccggag ggtctgatga agcaccgccc
     2161 aagcccagca gaccgggtta tcccagtccg aggtccagcg aaggatttta tcccagccca
     2221 cagcacatgg tacaaaccaa tcattaccag gtttctggct accctggttc acatggaatc
     2281 acagccatgg ctggcagcat ctatccaggt caggcatctc ttttggacca aacagattca
     2341 tggaatcata gacctcagga gatagcaatg tggcagccca atgtggagga ctctacagta
     2401 ttggacctgc gagggattgg gcaagtgttg ccaacccatc tgatggaaga gcgtctaatc
     2461 cgacagcaac aggaaatgga agaagatcag cgctggctgg aaaaagagga aagatttctg
     2521 aaacctgatg tgagactctc tcgaggcagt attgacaggg aggatggaag tcttcagggt
     2581 ccgattggaa accaacatat atatcagcct gtgggtaaac cagatcctgc agctccacca
     2641 aagaaaccgc ctcgccctgg agctcccggt catctgggaa gccttgccag cctcagcagc
     2701 cctgctgaca gctacaacga gggtgtcaag ccatggaggc ttcagcccca ggaaatcagc
     2761 ccccctccta ctgccaacct ggaccggtcg aatgataagg tgtacgagaa tgtgacgggc
     2821 ctggtgaaag ctgtcatcga gatgtccagt aaaatccagc cagccccacc agaggagtat
     2881 gtccctatgg tgaaggaagt cggcttggcc ctgaggacat tattggccac tgtggatgag
     2941 accattcccc tcctaccagc cagcacccac cgagagattg agatggcaca gaagctattg
     3001 aactctgacc tgggtgagct catcaacaag atgaaactgg cccagcagta tgtcatgacc
     3061 agcctccagc aagagtacaa aaagcaaatg ctgactgctg ctcacgccct ggctgtggat
     3121 gccaaaaact tactcgatgt cattgaccaa gcaagactga aaatgcttgg gcagacgaga
     3181 ccacacagct ccggagggga tccaccggtc gccaccatga gtgcgattaa gccagacatg
     3241 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
     3301 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
     3361 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
     3421 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     3481 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     3541 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     3601 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     3661 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     3721 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     3781 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     3841 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccggt
     3901 agcggtcacg gtactggttc tactggttct ggttcttctg agagtgcgat taagccagac
     3961 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
     4021 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
     4081 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
     4141 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat
     4201 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata
     4261 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc
     4321 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
     4381 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc
     4441 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
     4501 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
     4561 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgataa
     4621 gcggccgcga ctctagatca taatcagcca taccacatt
//
