LOCUS       sequence-88816-e        2151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2151)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             761..1435
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
       61 gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
      241 cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
      601 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggtag
      721 cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta agccagacat
      781 gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga
      841 tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg
      901 acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt
      961 cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc
     1021 gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac
     1081 aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa
     1141 tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt
     1201 gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca
     1261 ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg
     1321 ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt
     1381 taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg
     1441 actcagatct cgagccatgg ctagtcgagg cgcaacaaga cccaacgggc caaatactgg
     1501 aaataaaata tgccagttca aactagtact tctgggagag tctgctgttg gcaaatcaag
     1561 cctagtgctt cgttttgtga agggccaatt tcatgaattt caagagagta ccataggggc
     1621 tgcttttcta acccaaactg tgtgtcttga tgatacaaca gtaaagtttg aaatatggga
     1681 tacagctggt caagaacgat accatagctt agcaccaatg tactacagag gagcacaagc
     1741 agccatagtt gtatatgata tcacaaatga ggagtccttt gccagagcca aaaactgggt
     1801 taaagaactt cagaggcaag ccagtcctaa cattgtaata gctttatcag gaaacaaggc
     1861 tgatcttgca aataaaagag ctgtcgattt ccaggaagca cagtcctatg cagatgacaa
     1921 cagtttatta ttcatggaga catcagctaa aacatcgatg aatgtaaatg aaatattcat
     1981 ggcaatagct aaaaagttgc caaagaacga accacagaat ccaggagcaa attctgccag
     2041 aggaagagga gtagacctta ctgaacccac gcagccaacc aggaatcagt gttgtagtaa
     2101 ctaaggatcc accggatcta gataactgat cataatcagc cataccacat t
//
