LOCUS       sequence-88821-e        1839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1839)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..1839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..704
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             753..1427
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             1482..1769
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaacc
       61 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
      121 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
      181 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gccgaatatc
      241 cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
      301 gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg
      361 acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat ggtccagtta
      421 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
      481 tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat taccgatgtg
      541 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg
      601 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
      661 agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccggt agcggtcacg
      721 gtactggttc tactggttct ggttcttctg agagtgcgat taagccagac atgaagatca
      781 acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      841 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      901 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccgaat
      961 atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
     1021 gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata acaatggaag
     1081 gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc aatggtccag
     1141 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
     1201 gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc cattaccgat
     1261 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact
     1321 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
     1381 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat
     1441 ctggctccag cgcaggcagc gcatccggcg gaagcggaag ccctggtccg acccccagtg
     1501 gcactaacgt gggatcctca gggcgctctc ccagcaaagc agtggccgcc cgggcggcgg
     1561 gatccactgt ccggcagagg aaaaatgcca gctgtgggac aaggagtgca ggccgcacaa
     1621 cctcggcagg caccgggggg atgtggcgat tctacacaga agattcacct gggctcaaag
     1681 ttggccctgt tccagtattg gttatgagtc ttctgttcat cgcttctgta tttatgttgc
     1741 acatttgggg caagtacact cgttcgtagg aattctgcag tcgacggtac cgcgggcccg
     1801 ggatccaccg gatctagata actgatcata atcagccat
//
