LOCUS       sequence-88825-e        7069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Fang Z, Takizawa N, Wilson KA, Smith TC, Delprato A, Davidson MW, 
            Lambright DG, Luna EJ
  TITLE     The membrane-associated protein, supervillin, accelerates 
            F-actin-dependent rapid integrin recycling and cell motility.
  JOURNAL   Traffic. 2010 Jun;11(6):782-99. doi: 
            10.1111/j.1600-0854.2010.01062.x. Epub 2010 Mar 17.
  PUBMED    20331534
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01062.x"; journalName: "Traffic"; date: 
            "2010-06"; volume: "11"; issue: "6"; pages: "782-99"
COMMENT     Sequence Label: tdEos-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..7069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             760..1434
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
      721 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
      781 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      841 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      901 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      961 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     1021 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     1081 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     1141 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     1201 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     1261 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     1321 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     1381 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
     1441 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccttcga tgctcagctt
     1501 ttagctgccc ttcctaaagt tgcagaatta agaaaaatct ttgaaccaaa gaggaaagaa
     1561 tttttagaaa tgaaaagaaa agaaagaatt gcccggcgct tagaaggaat tgaaaccgac
     1621 acgcagccca tcctcttgca gagctgcacg ggcttggtga ctcatcgcct gctggaggag
     1681 gacacaccca gatacatgcg tgccacagac ccggccagcc cgcacattgg tcgatcaaat
     1741 gaagaagaag aaacttcaga ttcttcacta gaaaagcaga ctcgatccaa acagtgcaca
     1801 gaaacctcag gcatccatgc tgactcgccc tacagttcag gcatcatgga cacccagagc
     1861 ctcgagtcca aagccgaaag aatcgccagg tacaaagcag agagaagacg gcagctggca
     1921 gaaaagtatg ggctcaccct ggatccggaa gcagactctg aaactccatc tcgatacagc
     1981 aggtccagaa aggaccccga ggctgcggag aaaaggggag taagaagcga gcgatcggcc
     2041 gagtccagca gagacgccgg ctcatcctac tccaggactg agctctcggg gctcaggacc
     2101 tgcgtggctg agtccaagga ctanggcctg caccggagtg acggtgtttc cgacacagag
     2161 gtgctgctca atgcagaaaa ccaaagacgc ggtcaagagc ccagtgccac cgggctggcc
     2221 cgagacctgc cccttgcagg ggaggtctcc tcttctttct cattctccgg gcgagactct
     2281 gccctcggtg aagtgccaag gtccccaaag gcagtgcaca gcctgcccag tccatcgcct
     2341 gggcagccgg cctccccaag ccactccacc agtgacctgc cactccctgc ggaggctcga
     2401 gccaggtcaa cttcaaattc agaaatgcca gctgccgagg atgaagaaaa ggtcgatgag
     2461 cgagcgaggc tgagcgtcgc tgctaagagg ctgctcttca gggagatgga gaagtcgttt
     2521 gacgagaaga gcgtcccaaa gcgccgctcc cgcaatgcgg ccgtggagca gaggctgcgg
     2581 cgcctccagg accggtccca cacgcagccc gtcaccaccg aggaggtggt cattgcagcc
     2641 acattgcagg cgtctgctca ccaaaaggca ctagccagag accagacaaa cgagagcaaa
     2701 gattctgctg agcagggaga acctgactcc tccactctga gcttagcgga gaagctggcc
     2761 ttgtttaaca aactatctca gccggtctca aaagccattt caacacggaa cagattagac
     2821 atgagacaga ggaggatgaa tgctcgttat caaacccagc cggtcacgct tggagaagtg
     2881 gaacaggtgc agagtggcaa gctcatggct ttctctccca ccattaacac ttccgtgtcc
     2941 accgtggcat ctaccgtccc ccccatgtat gcaggaaatc ttcggacaaa gccacttcca
     3001 gatgacagct ttggtgccac tgagcagaag tttgcttctt cgctagaaaa ctccgactcc
     3061 ccagttagaa gcatcctgaa atcccaaggc tggcagccct cggtcgaggg tgctgggagc
     3121 aaagcaatgt tgagagaatt tgaagagaca gaacgcaagg gaggcttgac aggtggagat
     3181 ggcggggtta caaagtatgg gtccttcgag gaagcagagc tgtcctaccc cgtcctcagc
     3241 agagtccggg agggagacaa ccataaagag gccatctatg cccttccgag gaaaggaagc
     3301 ctggagctcg cccatcctcc catcgcccag cttggtgatg acctgaagga attttccacc
     3361 ccaaagagca ccatgcaggc aagcccagac tggaaggaga ggcagctctt tgaagagaag
     3421 gtggacttgg agaatgtcac aaagaggaag ttttcgctga aagccgcgga gttcggggaa
     3481 cccacttctg agcagaccgg cgcggctgct gggaaaccgg ctgctccgac tgcaaccccg
     3541 gtgtcctgga agccgcagga tccctccgaa cagcctcagg agaagcggta tcagagcccg
     3601 tgtgcgatgt ttgctgctgg agagatcaaa gccccggcgg tggagggcag cctcgactcc
     3661 cccagcaaaa ccatgtccat taaagaaaga ttagcactgt tgaagaagag cggtgaggaa
     3721 gactggagaa acagactcaa cagaaagcag gaatacggca aagcctccat caccagcagc
     3781 ctgcacatcc aagagacgga gcagtcactc aagaagaagc gggtcacaga aagtcgagag
     3841 agccagatga ctattgaaga gagaaagcat ctcatcaccg tgagagagga cgcctggaag
     3901 accaggggca aaggagcagc caacgactct acccagttta ctgtggccgg caggatggtg
     3961 aagcgaggtc tggcgtcccc cactgccatc accccggtag catcccctgt ttccagcaaa
     4021 gcaaggggca ctacaccagt ttccagaccc ctggaagata tcgaagccag accagacatg
     4081 cagttagagt cggacctcaa gctggacagg ctggaaacct tcctaagaag gctgaataac
     4141 aaagttggtg ggatgcaaga aacagtcctc actgtcactg ggaaatcggt gaaagaggtg
     4201 atgaagccgg acgatgatga aacctttgcc aagttttacc gcagcgtgga ctcctctcta
     4261 ccgagaagcc ccgtagagct ggacgaggat ttcgatgtca tttttgatcc ttatgccccc
     4321 aggttgacat cctcggtggc tgagcacaag cgtgccgtgc ggcccaagcg ccgggtccag
     4381 gcttccaaaa accccctgaa gatgctggcc gcgagggaag atctcctgca ggagtacact
     4441 gagcagagac tgaacgtggc cttcgtggag tcaaagcgga tgaaagtcga aaagttgtcc
     4501 gccaactcca gcttctcaga agtcaccctg gcggggttag ccagcaaaga aaacttcagc
     4561 aacgtcagcc tgcggagcgt caacctgacg gaacagaatt ccaacaacag tgcagtgccc
     4621 tacaagaagc tgatgctgct gcaggttaaa ggaagaagac acgtgcagac gcggctggtg
     4681 gagcctcgcg ccccctccct caacagcggg gactgcttcc tcctgctctc gccccatcac
     4741 tgcttcctgt gggtcggaga gttcgccaac gtgatcgaga aggcgaaggc ctcagaactt
     4801 gcgagtttaa ttcagaccaa gagggaactt ggttgtagag caacttacat ccagactgtt
     4861 gaagaaggaa ttaatacaca cacacatgca gccaaagact tctggaagct tctgggcggc
     4921 caagccagtt accaatccgc cggagaccca aaggaggatg agctctatga aacggccata
     4981 atagagacca actgcattta ccgtctgatg gatgacaaac tcgttcctga tgatgactac
     5041 tgggggaaga tcccaaagtg ctccctcctg caatcaaaag aggtactggt gtttgatttt
     5101 gggagtgaag tttacgtgtg gcatggaaaa gaagtcacat tagcacaacg gaaaatcgcc
     5161 tttcagctgg caaagcactt gtggaatgga acctttgact acgagaattg tgacattaac
     5221 ccgctggatc ctggggaatg caatcccctc attcccagga aaggacaggg gcggcccgac
     5281 tgggcaatat ttgggagact tacagaacac aatgagacga tcttgttcaa agagaaattc
     5341 cttgattgga cggaactgaa gagacctaac gagaagaacg ccagcgaact cgcgcagcac
     5401 aaggacgatg cccgggcaga ggtcaagcct tacgacgtga cgcggatggt gcccgtgccc
     5461 cagacaacag ccggcaccgt gctggatggg gtcaacgtgg gccgaggcta cgggctggtg
     5521 gaaggggacg accggaggca gttcgagatc gccagcatct cagtggacgt ctggcacatc
     5581 ctggagttcg actacagcag gctccccaag cagagcatcg ggcagttcca cgaaggcgat
     5641 gcctacgtgg tgaagtggaa gttcatcgtg agcactgcag tgggaagccg gcagaagggg
     5701 gagcactcgg tcagggtggc tggcaaagag aaatgtgtct acttcttctg gcaaggccgc
     5761 cagtcgaccg tgagtgagaa gggcacatca gctctgatga cagtggagct ggacgaggaa
     5821 aggggggccc aggtccaggt cctgcagggc aaggagcccc cctgtttcct gcagtgcttc
     5881 caggggggga tggtggtcca ctccgggaga cgggaggagg aggaagagaa cacacagagc
     5941 gagtggcggc tgtactgtgt gcgcggagag gtgcctgtgg aggggaactt gctggaggtg
     6001 gcctgtcact gcagcagcct caggtctagg acgtccatgg ttgtcctcaa cgtccacaaa
     6061 gccctcatct acctgtggca tggatgcaaa gcccaggccc acacgaagga ggtcggacgg
     6121 actgcagcca ataaaatcaa ggaccaatgt cccctggagg ccgggctgca cagtagcagc
     6181 aaagtgacaa ttcatgagtg tgatgaaggc tcggagcccc tggggttctg ggacgctctg
     6241 gggaggagag accggaaagc ctacgactgc atgcttcaag atcctggaaa ttttaacttc
     6301 acaccccgcc tgttcatcct cagcagctcc tctggtgact tctcagccac agagttcatg
     6361 tatcccgccc gagacccctc tgtggtcaac tctatgccct tcctgcagga agacctatac
     6421 agtgccccac agccagcact cttccttgtt gacaatcacc acgaggtgta cctctggcaa
     6481 ggctggtggc ccatcgagaa caagatcacg ggctccgcgc gcatccgctg ggcctcggac
     6541 cggaagagcg ccatggagac agtgctgcag tactgccgag ggaaaaacct gaagaagccg
     6601 ccccccaagt cttacctcat ccacgctggc ctggaacccc tgaccttcac caacatgttc
     6661 cccagctggg agcacagaga agacattgca gagatcacgg agatggacac ggaagtttca
     6721 aatcagatca ccctagtgga ggatgtctta gccaaactgt gtaagaccat ttacccactg
     6781 gccgacctcc tagccaggcc gctccctgag ggagttgacc ctctgaagct ggagatctat
     6841 ctcaccgacg aggacttcga gtttgcacta gacatgaccc gagacgagta caatgcactg
     6901 cccgcttgga agcaggtgaa cctgaagaaa gcgaaaggtc tgttctgagt caggagatgc
     6961 tggaggaacc tgggtggtca ctttcaatac tgatggacca ggaaaatgga tgtttaatcg
     7021 aattcccgcg gccttctaga taactgatca taatcagcca taccacatt
//
