LOCUS       sequence-88826-e        9167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: tdEos-Talin-18
FEATURES             Location/Qualifiers
     source          1..9167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..705
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             754..1428
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     regulatory      1477..1486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac
       61 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      121 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
      181 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat
      241 ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      301 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
      361 gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt
      421 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      481 gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt
      541 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
      601 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      661 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac
      721 ggtactggtt ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc
      781 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      841 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
      901 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
      961 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1021 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     1081 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
     1141 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     1201 ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga
     1261 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     1321 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     1381 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc cggactcaga
     1441 tctcgagctc aagctagctt cgaattcgca gaagctgcca ccatggttgc gctttcgctg
     1501 aagattagca ttgggaatgt ggtgaagacg atgcaatttg agccatctac catggtgtac
     1561 gacgcctgcc gcatgattcg tgagcggatc ccagaggccc tggctggccc tcccaacgac
     1621 tttgggctct ttctgtcaga tgatgacccc aaaaaaggca tctggctaga ggctgggaaa
     1681 gctttggact actacatgct ccgaaatggg gacaccatgg agtacagaaa aaagcagaga
     1741 cccctgaaga tacggatgtt agacggaacg gtgaagacta tcatggtgga tgactccaag
     1801 accgtcacag acatgctcat gaccatttgt gcccgaattg gtatcaccaa ccatgatgag
     1861 tattcactgg ttcgagagct gatggaagaa aagaaagatg aggggacagg gaccctgaga
     1921 aaagacaaga ccctactgcg agatgaaaag aagatggaga agctaaagca gaagttgcac
     1981 acagacgatg agttgaactg gctggaccat gggcggacac tgagggaaca gggagtggaa
     2041 gagcatgaga cgttgctgct gcggagaaag ttcttctact cagaccagaa tgtggattcc
     2101 cgagaccctg tacagctgaa ccttctctat gtgcaggcac gagatgacat cctgaatggc
     2161 tcccatcctg tctcctttga caaggcctgt gaatttgcag gcttccagtg ccagatccag
     2221 tttggacctc acaatgaaca gaagcacaag gctggcttcc ttgacctgaa ggacttcctg
     2281 cccaaggagt atgtgaagca gaagggagag cgtaagatct ttcaggcaca caagaattgt
     2341 gggcagatga gtgaaattga ggccaaggta cgctacgtga agctggcccg ttccctcaag
     2401 acttacggtg tctccttctt cctagtcaag gaaaagatga aggggaagaa taaactggtg
     2461 cccaggctgt tgggcatcac taaggagtgt gtgatgcgtg tggatgagaa gaccaaggag
     2521 gtgattcagg aatggagcct caccaacatc aaacgctggg ctgcctctcc caagagcttt
     2581 actctggact ttggagacta tcaggatggc tactactcag tacagacgac agaaggcgag
     2641 cagatcgcac agctcattgc tggctacata gatatcatcc ttaagaagaa aaaaagcaag
     2701 gaccattttg ggctggaggg agatgaagag tctactatgt tggaggactc agtgtctccc
     2761 aaaaagtcaa cagtccttca gcagcagtac aaccgagtgg ggaaagtgga gcacggctct
     2821 gtggctctgc cagccatcat gcgctctgga gcctctggtc ctgagaattt ccaggtgggg
     2881 agcatgccac ctgcccagca gcagatcacc agtggccaga tgcaccgagg acacatgcca
     2941 cctctgactt cagcccagca ggcgctcact ggaaccatta actccagcat gcaggctgtg
     3001 caggctgccc aggccactct ggatgacttt gagactctac cccctcttgg ccaggatgct
     3061 gcctccaaag cttggcgtaa gaacaagatg gatgaatcaa agcatgagat ccactcccag
     3121 gtagatgcca tcacagctgg cactgcctct gtggtaaacc tgacagcagg agatcctgca
     3181 gagacagact atacagcagt gggctgtgca gtcaccacca tctcttccaa cctgacggag
     3241 atgtcccgtg gggtgaagct attggctgcc ctgctggagg atgaaggcgg caatggccgg
     3301 ccccttctgc aagcagcaaa gggccttgca ggggctgtgt cggaactgct tcgcagtgct
     3361 cagcccgcca gcgctgagcc tcgtcagaac ctgctgcagg cagccgggaa cgtgggccag
     3421 gccagtgggg agctgttgca gcaaattggg gaaagtgaca ctgaccccca cttccaggat
     3481 gttctaatgc agctagcaaa cgcagtggca agtgctgcag ccgccctggt cctcaaggcc
     3541 aagagtgtgg cccagcgcac agaggattcc gggcttcaga cccaagtgat tgctgcagct
     3601 acgcaatgtg ctctgtccac ttcccagctg gtggcctgta ccaaggtggt agcacctaca
     3661 atcagctcac ctgtgtgcca agaacagcta gtcgaggctg ggcgactggt ggccaaagct
     3721 gtggagggct gtgtgtctgc ctcgcaagca gctacagaag atggacagct gcttcgaggg
     3781 gtaggagcag cagccacggc tgtcacccag gccctcaatg agctgctgca gcacgtgaag
     3841 gcccacgcca ccggagctgg gcctgccggt cggtatgacc aagccactga taccatcctc
     3901 accgtcactg aaaacatctt cagctccatg ggtgatgctg gggagatggt gcgccaggcc
     3961 cgcatcctgg cccaagccac ctcagacctg gtcaatgcta tcaaggccga tgctgagggg
     4021 gagagtgatc tggagaactc tagaaagctc ctgagtgctg ccaagatcct cgctgatgcc
     4081 accgccaaga tggtggaggc ggccaaggga gcagccgccc accctgacag tgaggaacag
     4141 cagcagcgac tgcgtgaagc agctgagggg cttcgcatgg ccaccaatgc agctgcgcag
     4201 aacgccatca agaagaagtt ggtgcagcgc ctggagcatg cagccaagca agctgcagcc
     4261 tctgcaacac agaccattgc tgcagcccaa catgcagcct ctgcccccaa ggcctctgcg
     4321 ggcccccagc ccctactggt gcagagctgt aaggccgtgg cagagcagat tccgttgctg
     4381 gtgcagggtg tccgaggaag tcaagctcag cctgacagcc ctagtgctca gctcgccctc
     4441 attgctgcca gccagagctt cctgcagcca ggtgggaaga tggtggcagc ggcaaaagcc
     4501 tcagtgccaa caattcagga ccaggcttca gcgatgcagc tgagtcagtg cgcaaagaac
     4561 ctaggcactg ccctggccga gctgcgcact gctgctcaga aggctcagga ggcatgtgga
     4621 cctttggaga tggattctgc actgagtgtt gtacaaaatc tagagaaaga cctacaggaa
     4681 ataaaagcag cagctcgaga tggcaagctt aagcccttac ctggggaaac aatggagaag
     4741 tgtacccaag atcttggcaa cagcaccaaa gcggtgagct ctgccatcgc caagctgctg
     4801 ggggagattg cccagggcaa cgagaactat gcaggtattg cagctcggga tgtagctggt
     4861 ggactaaggt cactagccca ggctgcgcgg ggtgtggctg cgctgacatc agatcctgca
     4921 gtgcaggcca ttgtgcttga cacagccagc gacgttctgg acaaggccag cagcctcatt
     4981 gaagaggcaa aaaaagcatc tggacaccca ggggacccag aaagccagca aaggcttgct
     5041 caggtagcta aagcagtgac ccaagccctg aaccgctgtg tcagctgcct gcctggccaa
     5101 agagacgtgg ataatgccct acgagccgtt ggagatgcca gcaagcgact cctgagtgac
     5161 ttgcttcctc caagcacggg gacatttcaa gaagcacaga gccgattgaa tgaagctgca
     5221 gctgggttaa atcaggccgc cacagaactg gtgcaagcct cccgaggaac tcctcaggac
     5281 ctggctcggg cctccggccg atttggacag gacttcagca ccttcctgga agctggtgtg
     5341 gagatggccg gacaggcccc gagccaggag gaccgagccc aggtggtgtc caacctgaag
     5401 ggcatatcca tgtcttcaag caaacttctc cttgccgcta aggccttgtc cacagaccct
     5461 gcttctccca acctcaagag tcagctggct gcggctgccc gggcagtgac ggacagcatc
     5521 aaccagctca tcaccatgtg cacccagcag gcacctggcc agaaggagtg tgacaatgca
     5581 cttcggcagc tagagacagt ccgagaactc ctggagaatc cagtccagcc catcaacgac
     5641 atgtcctact tcggttgctt ggacagtgtc atggagaact ccaaggtcct aggtgaggcc
     5701 atgactggca tctcccaaaa tgccaagaat ggaaatctgc cggagtttgg agatgccatt
     5761 gccacagcct ccaaggctct ctgtggcttc acggaggcag ccgcacaggc agcatatcta
     5821 gttggtgtct ctgaccccaa cagccaagct ggacagcaag gactggtgga acccacacag
     5881 tttgcccgtg caaaccaggc aattcagatg gcctgtcaga gtctggggga gcctggctgt
     5941 acccaggccc aggtgttatc tgcagccact attgtagcca aacacacatc tgcattgtgt
     6001 aacagctgtc gcctggcttc cgctaggact gccaatccca ctgccaagcg ccagtttgta
     6061 cagtcagcca aggaggtggc caacagtaca gccaatcttg tcaagaccat caaggcacta
     6121 gatggggact tcacagaaga gaaccgtgcc cagtgccgag cagccacagc ccctctgctg
     6181 gaagctgtgg ataacctgag tgcctttgcc tccaaccctg agttctccag cgtccctgcc
     6241 cagatcagcc ctgagggcag agcagccatg gagcccattg taatctctgc taagacaatg
     6301 ttggagagtg ctggagggct catccagaca gctcgggcgt tagcagtcaa tccccgagac
     6361 cccccacgtt ggtctgtgct agctggccac tctcggactg tctcagattc catcaagaaa
     6421 cttattacaa gcatgaggga caaagcccca gggcagctgg agtgtgagac agccattgcg
     6481 gctctgaaca gctgcttgcg ggacctagat caggcttcgc ttgccgctgt cagccagcag
     6541 cttgctcccc gtgaaggaat ctctcaagag gctttgcaca cccagatgct cactgcagtg
     6601 caagaaattt ctcatctcat tgagccgctg gccagcgctg ctcgggctga agcctcccag
     6661 ctgggacaca aggtgtccca aatggcccag tactttgaac cactcaccct ggctgcagtg
     6721 ggtgctgcgt ctaagaccct gagccacccg cagcagatgg cacttctgga ccagactaaa
     6781 acgttggcag agtctgcctt gcagttgcta tatactgcca aggaggctgg tggtaacccc
     6841 aagcaagcag cacacaccca ggaagccctg gaggaggctg tgcagatgat gacagaggcc
     6901 gtagaagacc tgacgacgac cctcaacgaa gcagccagtg ctgcaggagt cgttggcggc
     6961 atggtggact ctatcactca ggccatcaac cagctagatg aaggacctat gggtgaccca
     7021 gaaggctcat tcgtagatta ccagacaacc atggtgagga cagccaaggc cattgctgtc
     7081 actgttcagg agatggtaac caagtcaaac accagccctg aagagctagg ccctcttgcc
     7141 aaccagctga ccagtgacta tggccgactg gcctcacaag ccaagcctgc agctgtggct
     7201 gctgaaaatg aagagatagg cgctcacatc aaacaccgag tacaggagct gggccatggc
     7261 tgctctgctc tggtcaccaa ggcaggtgcc ttgcagtgta gccccagtga tgtctacacc
     7321 aagaaggagc tcatagagtg tgcccgcaga gtgtcagaga aggtctccca tgttctggct
     7381 gcactccagg ctgggaatcg tggtacccag gcctgcatta cagcagccag tgctgtgtct
     7441 ggtatcattg ctgacctcga caccaccatc atgttcgcta ctgctggcac acttaaccgt
     7501 gagggtgctg aaacttttgc tgaccaccgg gagggtatct taaagacagc aaaggttctt
     7561 gtggaggaca ccaaggtcct agtgcagaat gcagctggga gccaggagaa gttggcacaa
     7621 gccgcccagt cctccgtggc caccattacc cgcctcgctg atgtggtcaa gctcggtgca
     7681 gccagcctag gagccgaaga ccctgaaact caggtggtgc tgatcaatgc agtaaaggac
     7741 gtagccaagg ccctgggtga cctcatcagc gctacgaagg ctgcagcggg caaagttggg
     7801 gatgaccctg cagtgtggca gctcaagaac tctgccaagg tgatggtgac caatgtgaca
     7861 tcattgctca agacagtgaa ggctgtggaa gatgaggcca ccaaaggcac acgggcccta
     7921 gaggcaacca cagagcacat acgtcaggaa ctggcggtct tctgttcccc agagccacct
     7981 gccaagacct ctacccctga agacttcatc cgaatgacca agggtattac tatggcaaca
     8041 gccaaagccg ttgctgctgg caattcctgt cgacaggaag atgtcattgc cacagccaat
     8101 ctgagccgac gggctattgc ggacatgctt cgggcttgca aggaagcagc tttccaccca
     8161 gaagtggcgc ctgatgtacg gctccgagcc ctgcactatg gccgggagtg tgccaatggt
     8221 tacctggaac tgctggacca cgtgctgctg acccttcaga agccaaaccc agacctgaag
     8281 cagcagctaa ctggacactc aaagcgggtt gctggctctg tgactgagct catccaggct
     8341 gctgaagcca tgaaaggaac agagtgggtg gacccagagg accctactgt cattgctgag
     8401 aatgagctct tgggagccgc agccgccatc gaggctgcag ccaagaagct ggagcagctg
     8461 aagcccaggg ccaaacccaa ggaggcggat gagtccttga actttgagga acaaatccta
     8521 gaagctgcca agtccatcgc tgcagccacc agtgcactgg taaaggctgc gtcagcagcc
     8581 cagagggagc tggtggctca aggaaaggtg ggcgccattc cagccaatgc actggatgat
     8641 gggcagtggt cgcagggcct catttccgct gcccgtatgg tggctgcggc caccaacaat
     8701 ctgtgtgaag cagctaacgc agctgtccag ggccatgcta gccaggagaa actcatttcc
     8761 tcagccaagc aagtagctgc ctccacagct cagctcctgg tagcttgcaa ggtcaaagca
     8821 gatcaggact ctgaggcaat gaaacggctc caggctgctg gcaatgcagt gaagagggcc
     8881 tcagataacc tggtaaaggc ggcccagaaa gctgcagcct tcgaagacca ggagaatgag
     8941 acggtggtgg tgaaggagaa aatggttggg ggcattgccc agattatcgc agcacaggaa
     9001 gagatgcttc ggaaggaacg agagctggaa gaggctcgga aaaagctcgc ccagatccgg
     9061 cagcagcagt acaagttctt gccttcagag cttcgagacg agcactaaaa aagccctgtg
     9121 tatttaatgc agacccagcc cagagactgt gcctgccact accaaag
//
