LOCUS       sequence-88829-e        2847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2847)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Talin-H-18
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..700
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             749..1423
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     regulatory      1472..1481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg
       61 tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc
      121 ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc
      181 ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga
      241 ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt
      301 gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac
      361 tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca
      421 gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct
      481 gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt
      541 cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga
      601 ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca
      661 tgctgttgct cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac
      721 tggttctact ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct
      781 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
      841 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt
      901 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc
      961 agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
     1021 cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga
     1081 cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat
     1141 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
     1201 gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga
     1261 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt
     1321 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
     1381 gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggac tcagatctcg
     1441 agctcaagct agcttcgaat tcgcagaagc tgccaccatg gttgcgcttt cgctgaagat
     1501 tagcattggg aatgtggtga agacgatgca atttgagcca tctaccatgg tgtacgacgc
     1561 ctgccgcatg attcgtgagc ggatcccaga ggccctggct ggccctccca acgactttgg
     1621 gctctttctg tcagatgatg accccaaaaa aggcatctgg ctagaggctg ggaaagcttt
     1681 ggactactac atgctccgaa atggggacac catggagtac agaaaaaagc agagacccct
     1741 gaagatccgg atgttagacg gaacggtgaa gactatcatg gtggatgact ccaagaccgt
     1801 cacagacatg ctcatgacca tttgtgcccg aattggtatc accaaccatg atgagtattc
     1861 actggttcga gagctgatgg aagaaaagaa agatgagggg acagggaccc tgagaaaaga
     1921 caagacccta ctgcgagatg aaaagaagat ggagaagcta aagcagaagt tgcacacaga
     1981 cgatgagttg aactggctgg accatgggcg gacactgagg gaacagggag tggaagagca
     2041 tgagacgttg ctgctgcgga gaaagttctt ctactcagac cagaatgtgg attcccgaga
     2101 ccctgtacag ctgaaccttc tctatgtgca ggcacgagat gacatcctga atggctccca
     2161 tcctgtctcc tttgacaagg cctgtgaatt tgcaggcttc cagtgccaga tccagtttgg
     2221 acctcacaat gaacagaagc acaaggctgg cttccttgac ctgaaggact tcctgcccaa
     2281 ggagtatgtg aagcagaagg gagagcgtaa gatctttcag gcacacaaga attgtgggca
     2341 gatgagtgaa attgaggcca aggtacgcta cgtgaagctg gcccgttccc tcaagactta
     2401 cggtgtctcc ttcttcctag tcaaggaaaa gatgaagggg aagaataaac tggtgcccag
     2461 gctgttgggc atcactaagg agtgtgtgat gcgtgtggat gagaagacca aggaggtgat
     2521 tcaggaatgg agcctcacca acatcaaacg ctgggctgcc tctcccaaga gctttactct
     2581 ggactttgga gactatcagg atggctacta ctcagtacag acgacagaag gcgagcagat
     2641 cgcacagctc attgctggct acatagatat catccttaag aagaaaaaaa gcaaggacca
     2701 ttttgggctg gagggagatg aagagtctac tatgttggag gactcagtgt ctcccaaaaa
     2761 gtcaacagtc cttcagtaag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     2821 actgatcata atcagccata ccacatt
//
