LOCUS       sequence-88830-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Talin-IBS2-7
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..798
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             847..1524
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgggc caggtggtgc tgatcaacgc cgtgaaggac
       61 gtggccaagg ccctgggcga cctgatcagc gccaccaagg gggatccacc ggtcgccacc
      121 atgagtgcga ttaagccaga catgaagatc aacctccgta tggaaggcaa cgtaaacggg
      181 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
      241 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
      301 ttccattacg gcaacagggt attcgccgaa tatccagacc acatacaaga ctattttaag
      361 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
      421 tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
      481 cacggtgtaa actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
      541 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga ctggtgatat tcgcatggct
      601 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
      661 gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc
      721 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
      781 cctgacaatg ccagacgatc cggtagcggt cacggtactg gttctactgg ttctggttct
      841 tctgagagtg cgattaagcc agacatgaag atcaacctcc gtatggaagg caacgtaaac
      901 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttttgaggg aaaacagagt
      961 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
     1021 gcattccatt acggcaacag ggtattcgcc gaatatccag accacataca agactatttt
     1081 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc
     1141 atttgcattg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
     1201 tttcacggtg taaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
     1261 gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgactggtga tattcgcatg
     1321 gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct
     1381 aaggagaagg gtgtcaagtt accaggctac cactttgtgg accactgcat tgagatttta
     1441 agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga
     1501 ttgcctgaca atgccagacg ataagcggcc gcgactctag atcataatca gccataccac
     1561 at
//
