LOCUS       sequence-88831-e        7881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7881)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             760..1434
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggtagc
      721 ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa gccagacatg
      781 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
      841 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
      901 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
      961 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     1021 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     1081 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     1141 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     1201 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     1261 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     1321 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     1381 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccgga
     1441 ctcagatctc gagctcaagc tagcttcgaa ttcgccacca tgcagcagta caaccgagtg
     1501 gggaaagtgg agcacggctc tgtggctctg ccagccatca tgcgctctgg agcctctggt
     1561 cctgagaatt tccaggtggg gagcatgcca cctgcccagc agcagatcac cagtggccag
     1621 atgcaccgag gacacatgcc acctctgact tcagcccagc aggcgctcac tggaaccatt
     1681 aactccagca tgcaggctgt gcaggctgcc caggccactc tggatgactt tgagactcta
     1741 ccccctcttg gccaggatgc tgcctccaaa gcttggcgta agaacaagat ggatgaatca
     1801 aagcatgaga tccactccca ggtagatgcc atcacagctg gcactgcctc tgtggtaaac
     1861 ctgacagcag gagatcctgc agagacagac tatacagcag tgggctgtgc agtcaccacc
     1921 atctcttcca acctgacgga gatgtcccgt ggggtgaagc tattggctgc cctgctggag
     1981 gatgaaggcg gcaatggccg gccccttctg caagcagcaa agggccttgc aggggctgtg
     2041 tcggaactgc ttcgcagtgc tcagcccgcc agcgctgagc ctcgtcagaa cctgctgcag
     2101 gcagccggga acgtgggcca ggccagtggg gagctgttgc agcaaattgg ggaaagtgac
     2161 actgaccccc acttccagga tgttctaatg cagctagcaa acgcagtggc aagtgctgca
     2221 gccgccctgg tcctcaaggc caagagtgtg gcccagcgca cagaggattc cgggcttcag
     2281 acccaagtga ttgctgcagc tacgcaatgt gctctgtcca cttcccagct ggtggcctgt
     2341 accaaggtgg tagcacctac aatcagctca cctgtgtgcc aagaacagct agtcgaggct
     2401 gggcgactgg tggccaaagc tgtggagggc tgtgtgtctg cctcgcaagc agctacagaa
     2461 gatggacagc tgcttcgagg ggtaggagca gcagccacgg ctgtcaccca ggccctcaat
     2521 gagctgctgc agcacgtgaa ggcccacgcc accggagctg ggcctgccgg tcggtatgac
     2581 caagccactg ataccatcct caccgtcact gaaaacatct tcagctccat gggtgatgct
     2641 ggggagatgg tgcgccaggc ccgcatcctg gcccaagcca cctcagacct ggtcaatgct
     2701 atcaaggccg atgctgaggg ggagagtgat ctggagaact ctagaaagct cctgagtgct
     2761 gccaagatcc tcgctgatgc caccgccaag atggtggagg cggccaaggg agcagccgcc
     2821 caccctgaca gtgaggaaca gcagcagcga ctgcgtgaag cagctgaggg gcttcgcatg
     2881 gccaccaatg cagctgcgca gaacgccatc aagaagaagt tggtgcagcg cctggagcat
     2941 gcagccaagc aagctgcagc ctctgcaaca cagaccattg ctgcagccca acatgcagcc
     3001 tctgccccca aggcctctgc gggcccccag cccctactgg tgcagagctg taaggccgtg
     3061 gcagagcaga ttccgttgct ggtgcagggt gtccgaggaa gtcaagctca gcctgacagc
     3121 cctagtgctc agctcgccct cattgctgcc agccagagct tcctgcagcc aggtgggaag
     3181 atggtggcag cggcaaaagc ctcagtgcca acaattcagg accaggcttc agcgatgcag
     3241 ctgagtcagt gcgcaaagaa cctaggcact gccctggccg agctgcgcac tgctgctcag
     3301 aaggctcagg aggcatgtgg acctttggag atggattctg cactgagtgt tgtacaaaat
     3361 ctagagaaag acctacagga aataaaagca gcagctcgag atggcaagct taagccctta
     3421 cctggggaaa caatggagaa gtgtacccaa gatcttggca acagcaccaa agcggtgagc
     3481 tctgccatcg ccaagctgct gggggagatt gcccagggca atgagaacta tgcaggtatt
     3541 gcagctcggg atgtagctgg tggactaagg tcactagccc aggctgcgcg gggtgtggct
     3601 gcgctgacat cagatcctgc agtgcaggcc attgtgcttg acacagccag cgacgttctg
     3661 gacaaggcca gcagcctcat tgaagaggca aaaaaagcat ctggacaccc aggggaccca
     3721 gaaagccagc aaaggcttgc tcaggtagct aaagcagtga cccaagccct gaaccgctgt
     3781 gtcagctgcc tgcctggcca aagagacgtg gataatgccc tacgagccgt tggagatgcc
     3841 agcaagcgac tcctgagtga cttgcttcct ccaagcacgg ggacatttca agaagcacag
     3901 agccgattga atgaagctgc agctgggtta aatcaggccg ccacagaact ggtgcaagcc
     3961 tcccgaggaa ctcctcagga cctggctcgg gcctccggcc gatttggaca ggacttcagc
     4021 accttcctgg aagctggtgt ggagatggcc ggacaggccc cgagccagga ggaccgagcc
     4081 caggtggtgt ccaacctgaa gggcatatcc atgtcttcaa gcaaacttct ccttgccgct
     4141 aaggccttgt ccacagaccc tgcttctccc aacctcaaga gtcagctggc tgcggctgcc
     4201 cgggcagtga cggacagcat caaccagctc atcaccatgt gcacccagca ggcacctggc
     4261 cagaaggagt gtgacaatgc acttcggcag ctagagacag tccgagaact cctggagaat
     4321 ccagtccagc ccatcaacga catgtcctac ttcggttgct tggacagtgt catggagaac
     4381 tccaaggtcc taggtgaggc catgactggc atctcccaaa atgccaagaa tggaaatctg
     4441 ccggagtttg gagatgccat tgccacagcc tccaaggctc tctgtggctt cacggaggca
     4501 gccgcacagg cagcatatct agttggtgtc tctgacccca acagccaagc tggacagcaa
     4561 ggactggtgg aacccacaca gtttgcccgt gcaaaccagg caattcagat ggcctgtcag
     4621 agtctggggg agcctggctg tacccaggcc caggtgttat ctgcagccac tattgtagcc
     4681 aaacacacat ctgcattgtg taacagctgt cgcctggctt ccgctaggac tgccaatccc
     4741 actgccaagc gccagtttgt acagtcagcc aaggaggtgg ccaacagtac agccaatctt
     4801 gtcaagacca tcaaggcact agatggggac ttcacagaag agaaccgtgc ccagtgccga
     4861 gcagccacag cccctctgct ggaagctgtg gataacctga gtgcctttgc ctccaaccct
     4921 gagttctcca gcgtccctgc ccagatcagc cctgagggca gagcagccat ggagcccatt
     4981 gtaatctctg ctaagacaat gttggagagt gctggagggc tcatccagac agctcgggcg
     5041 ttagcagtca atccccgaga ccccccacgt tggtctgtgc tagctggcca ctctcggact
     5101 gtctcagatt ccatcaagaa acttattaca agcatgaggg acaaagcccc agggcagctg
     5161 gagtgtgaga cagccattgc ggctctgaac agctgcttgc gggacctaga tcaggcttcg
     5221 cttgccgctg tcagccagca gcttgctccc cgtgaaggaa tctctcaaga ggctttgcac
     5281 acccagatgc tcactgcagt gcaagaaatt tctcatctca ttgagccgct ggccagcgct
     5341 gctcgggctg aagcctccca gctgggacac aaggtgtccc aaatggccca gtactttgaa
     5401 ccactcaccc tggctgcagt gggtgctgcg tctaagaccc tgagccaccc gcagcagatg
     5461 gcacttctgg accagactaa aacgttggca gagtctgcct tgcagttgct atatactgcc
     5521 aaggaggctg gtggtaaccc caagcaagca gcacacaccc aggaagccct ggaggaggct
     5581 gtgcagatga tgacagaggc cgtagaagac ctgacgacga ccctcaacga agcagccagt
     5641 gctgcaggag tcgttggcgg catggtggac tctatcactc aggccatcaa ccagctagat
     5701 gaaggaccta tgggtgaccc agaaggctca ttcgtagatt accagacaac catggtgagg
     5761 acagccaagg ccattgctgt cactgttcag gagatggtaa ccaagtcaaa caccagccct
     5821 gaagagctag gccctcttgc caaccagctg accagtgact atggccgact ggcctcacaa
     5881 gccaagcctg cagctgtggc tgctgaaaat gaagagatag gcgctcacat caaacaccga
     5941 gtacaggagc tgggccatgg ctgctctgct ctggtcacca aggcaggtgc cttgcagtgt
     6001 agccccagtg atgtctacac caagaaggag ctcatagagt gtgcccgcag agtgtcagag
     6061 aaggtctccc atgttctggc tgcactccag gctgggaatc gtggtaccca ggcctgcatt
     6121 acagcagcca gtgctgtgtc tggtatcatt gctgacctcg acaccaccat catgttcgct
     6181 actgctggca cacttaaccg tgagggtgct gaaacttttg ctgaccaccg ggagggtatc
     6241 ttaaagacag caaaggttct tgtggaggac accaaggtcc tagtgcagaa tgcagctggg
     6301 agccaggaga agttggcaca agccgcccag tcctccgtgg ccaccattac ccgcctcgct
     6361 gatgtggtca agctcggtgc agccagccta ggagccgaag accctgaaac tcaggtggtg
     6421 ctgatcaatg cagtaaagga cgtagccaag gccctgggtg acctcatcag cgctacgaag
     6481 gctgcagcgg gcaaagttgg ggatgaccct gcagtgtggc agctcaagaa ctctgccaag
     6541 gtgatggtga ccaatgtgac atcattgctc aagacagtga aggctgtgga agatgaggcc
     6601 accaaaggca cacgggccct agagaagacc tctacccctg aagacttcat ccgaatgacc
     6661 aagggtatta ctatggcaac agccaaagcc gttgctgctg gcaattcctg tcgacaggaa
     6721 gatgtcattg ccacagccaa tctgagccga cgggctattg cggacatgct tcgggcttgc
     6781 aaggaagcag ctttccaccc agaagtggcg cctgatgtac ggctccgagc cctgcactat
     6841 ggccgggagt gtgccaatgg ttacctggaa ctgctggacc acgtgctgct gacccttcag
     6901 aagccaaacc cagacctgaa gcagcagcta actggacact caaagcgggt tgctggctct
     6961 gtgactgagc tcatccaggc tgctgaagcc atgaaaggaa cagagtgggt ggacccagag
     7021 gaccctactg tcattgctga gaatgagctc ttgggagccg cagccgccat cgaggctgca
     7081 gccaagaagc tggagcagct gaagcccagg gccaaaccca aggaggcgga tgagtccttg
     7141 aactttgagg aacaaatcct agaagctgcc aagtccatcg ctgcagccac cagtgcactg
     7201 gtaaaggctg cgtcagcagc ccagagggag ctggtggctc aaggaaaggt gggcgccatt
     7261 ccagccaatg cactggatga tgggcagtgg tcgcagggcc tcatttccgc tgcccgtatg
     7321 gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg cagctgtcca gggccatgct
     7381 agccaggaga aactcatttc ctcagccaag caagtagctg cctccacagc tcagctcctg
     7441 gtagcttgca aggtcaaagc agatcaggac tctgaggcaa tgaaacggct ccaggctgct
     7501 ggcaatgcag tgaagagggc ctcagataac ctggtaaagg cggcccagaa agctgcagcc
     7561 ttcgaagacc aggagaatga gacggtggtg gtgaaggaga aaatggttgg gggcattgcc
     7621 cagattatcg cagcacagga agagatgctt cggaaggaac gagagctgga agaggctcgg
     7681 aaaaagctcg cccagatccg gcagcagcag tacaagttct tgccttcaga gcttcgagac
     7741 gagcactaaa aaagccctgt gtatttaatg cagacccagc ccagagactg tgcctgccac
     7801 taccaaagcc ttctgggctg ctcaggaccc aagctgccca accccagcac tcccccaaag
     7861 tgcctgccaa accctgggcc t
//
