LOCUS       sequence-88834-e        2790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2790)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-TES-N-14
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1341..2015
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     CDS             2064..2741
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gcgatgctag tgacgtcaga tccgctagcg ccaccatgga cctggaaaac aaagtgaaga
       61 agatgggctt aggtcacgag caaggatttg gagccccttg tttaaaatgc aaagaaaaat
      121 gtgaaggatt cgaactgcac ttctggagaa aaatatgtcg taactgcaag tgtggccaag
      181 aagagcatga tgtcctcttg agcaatgaag aggatcgaaa agtgggaaaa ctttttgaag
      241 acaccaagta taccactctg attgcaaaac taaagtcaga tggaattccc atgtataaac
      301 gcaatgttat gatattgacg aatccagttg ctgccaagaa gaatgtctcc atcaatacag
      361 ttacctatga gtgggctcct cctgtccaga atcaagcatt ggccaggcag tacatgcaga
      421 tgctacccaa ggaaaagcag ccagtagcag gctcagaggg ggcacagtac cggaagaagc
      481 agctggcaaa gcagctccct gcacatgacc aggacccttc aaagtgccat gagttgtctc
      541 ccagagaggt gaaggagatg gagcagtttg tgaagaaata taagagcgaa gctctgggag
      601 taggagatgt caaacttccc tgtgagatgg atgcccaagg ccccaaacaa atgaacattc
      661 ctggagggga tagaagcacc ccagcagcag tgggggccat ggaggacaaa tctgctgagc
      721 acaaaagaac tcaatattcc tgctattgct gcaaactgag tatgaaagaa ggtgacccag
      781 ccatctatgc cgaaagggct ggctatgata aactgtggca cccagcttgt tttgtctgca
      841 gcacctgcca tgaactcctg gttgacatga tttatttttg gaagaatgag aagctatact
      901 gtggcagaca ttactgtgac agcgagaaac cccgatgtgc tggctgtgac gagctgatat
      961 tcagcaatga gtatacccag gcagaaaacc agaattggca cctgaaacac ttctgctgct
     1021 ttgactgtga tagcattcta gctggggaga tatacgtgat ggtcaatgac aagcccgtgt
     1081 gcaagccctg ctatgtgaag aatcacgctg tggtgtgtca aggatgccac aatgccatcg
     1141 acccagaagt gcagcgggtg acctataaca atttcagctg gcatgcatcc acagagtgct
     1201 ttctgtgctc ttgctgcagc aaatgcctca ttgggcagaa gttcatgcca gtagaaggga
     1261 tggttttctg ttcagtggaa tgtaagaaga ggatgtcttc cggcggtagc ggcggtagcg
     1321 gggatccacc ggtcgccacc agtgcgatta agccagacat gaagatcaac ctccgtatgg
     1381 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
     1441 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
     1501 atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat ccagaccaca
     1561 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
     1621 tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct
     1681 ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga
     1741 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgactg
     1801 gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
     1861 ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact
     1921 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     1981 ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac ggtactggtt
     2041 ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc aacctccgta
     2101 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
     2161 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
     2221 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa tatccagacc
     2281 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
     2341 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt
     2401 tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca gttatgcaga
     2461 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
     2521 ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
     2581 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc
     2641 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
     2701 ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc
     2761 ataatcagcc ataccacatt gagagaagcc
//
