LOCUS       sequence-88835-e        1926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1926)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             483..1160
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1209..1886
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccgctag cgccaccatg gtgggtcgga acagcgccat cgccgccggt gtatgcgggg
       61 cccttttcat tgggtactgc atctacttcg accgcaaaag acgaagtgac cccaacttca
      121 agaacaggct tcgagaacga agaaagaaac agaagcttgc caaggagaga gctgggcttt
      181 ccaagttacc tgaccttaaa gatgctgaag ctgttcagaa gttcttcctt gaagaaatac
      241 agcttggtga agagttacta gctcaaggtg aatatgagaa gggcgtagac catctgacaa
      301 atgcaattgc tgtgtgtgga cagccacagc agttactgca ggtcttacag caaactcttc
      361 caccaccagt gttccagatg cttctgacta agctcccaac aattagtcag agaattgtaa
      421 gtgctcagag cttggctgaa gatgatgtgg aaggcggtag cggggatcca ccggtcgcca
      481 ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc aacgtaaacg
      541 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta
      601 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg
      661 cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa gactatttta
      721 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca
      781 tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat
      841 ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg
      901 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat attcgcatgg
      961 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta
     1021 aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt gagattttaa
     1081 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat
     1141 tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact ggttctggtt
     1201 cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa ggcaacgtaa
     1261 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
     1321 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
     1381 ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata caagactatt
     1441 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
     1501 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
     1561 gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
     1621 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt gatattcgca
     1681 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
     1741 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
     1801 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
     1861 gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc
     1921 acattt
//
