LOCUS       sequence-88837-e        2882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW
  TITLE     Pathogenic mutation of spastin has gain-of-function effects on 
            microtubule dynamics.
  JOURNAL   J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 
            10.1523/JNEUROSCI.3309-13.2014.
  PUBMED    24478365
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3309-13"; journalName: "J Neurosci"; date: 
            "2014-01-29- 29"; volume: "34"; issue: "5"; pages: "1856-67"
COMMENT     Sequence Label: tdEos-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..705
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             754..1428
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 aatccgctag cgctaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac
       61 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      121 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
      181 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat
      241 ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      301 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
      361 gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt
      421 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      481 gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt
      541 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
      601 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      661 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac
      721 ggtactggtt ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc
      781 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      841 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
      901 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
      961 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1021 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     1081 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
     1141 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     1201 ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga
     1261 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     1321 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     1381 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc cggactcaga
     1441 tctggcagcg gtggaggcag cgcatccggc ggaagcggaa gcgtgcgtga gtgcatctcc
     1501 atccacgttg gccaggctgg tgtccagatt ggcaatgcct gctgggagct ctactgcctg
     1561 gaacacggca tccagcccga tggccagatg ccaagtgaca agaccattgg gggaggagat
     1621 gactccttca acaccttctt cagtgagacg ggcgctggca agcacgtgcc ccgggctgtg
     1681 tttgtagact tggaacccac agtcattgat gaagttcgca ctggcaccta ccgccagctc
     1741 ttccaccctg agcagctcat cacaggcaag gaagatgctg ccaataacta tgcccgaggg
     1801 cactacacca ttggcaagga gatcattgac cttgtgttgg accgaattcg caagctggct
     1861 gaccagtgca ccggtcttca gggcttcttg gttttccaca gctttggtgg gggaactggt
     1921 tctgggttca cctccctgct catggaacgt ctctcagttg attatggcaa gaagtccaag
     1981 ctggagttct ccatttaccc agcaccccag gtttccacag ctgtagttga gccctacaac
     2041 tccatcctca ccacccacac caccctggag cactctgatt gtgccttcat ggtagacaat
     2101 gaggccatct atgacatctg tcgtagaaac ctcgatatcg agcgcccaac ctacactaac
     2161 cttaaccgcc ttattagcca gattgtgtcc tccatcactg cttccctgag atttgatgga
     2221 gccctgaatg ttgacctgac agaattccag accaacctgg tgccctaccc ccgcatccac
     2281 ttccctctgg ccacatatgc ccctgtcatc tctgctgaga aagcctacca tgaacagctt
     2341 tctgtagcag agatcaccaa tgcttgcttt gagccagcca accagatggt gaaatgtgac
     2401 cctcgccatg gtaaatacat ggcttgctgc ctgttgtacc gtggtgacgt ggttcccaaa
     2461 gatgtcaatg ctgccattgc caccatcaaa accaagcgca gcatccagtt tgtggattgg
     2521 tgccccactg gcttcaaggt tggcatcaac taccagcctc ccactgtggt gcctggtgga
     2581 gacctggcca aggtacagag agctgtgtgc atgctgagca acaccacagc cattgctgag
     2641 gcctgggctc gcctggacca caagtttgac ctgatgtatg ccaagcgtgc ctttgttcac
     2701 tggtacgtgg gtgaggggat ggaggaaggc gagttttcag aggcccgtga agatatggct
     2761 gcccttgaga aggattatga ggaggttggt gtggattctg ttgaaggaga gggtgaggaa
     2821 gaaggagagg aatactaagg atccaccgga tctagataac tgatcataat cagccatacc
     2881 ac
//
