LOCUS       sequence-88839-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-VASP-5
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..707
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             756..1430
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
       61 acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
      121 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
      181 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccgaat
      241 atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
      301 gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata acaatggaag
      361 gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc aatggtccag
      421 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
      481 gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc cattaccgat
      541 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact
      601 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
      661 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggtagcggtc
      721 acggtactgg ttctactggt tctggttctt ctgagagtgc gattaagcca gacatgaaga
      781 tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
      841 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
      901 tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg
      961 aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
     1021 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
     1081 aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc
     1141 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
     1201 atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc
     1261 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
     1321 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
     1381 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga tccggactca
     1441 gatccatgag cgagacggtc atctgttcca gccgggctac tgtgatgctt tatgatgaca
     1501 gcaacaagcg atggctccct gctggcactg gtccccaggc cttcagccgc gtccagatct
     1561 accacaaccc cactgctaac tccttccgag ttgttggccg caagatgcag ccggatcagc
     1621 aggtggttat caactgtgcc atcattcggg gtgtcaagta caatcaggcc actcccatct
     1681 tccatcagtg gcgagatgcc cgccaggtct ggggcctcaa cttcggcagc aaggaggacg
     1741 ccatacagtt tgcaacaggc atggccaacg ccctagaggc cttggaagga ggtgggcctc
     1801 ccccagcccc agcaccccct gcctggtctg cccagaatgg tccctcccca gaggagctgg
     1861 aacaacagaa aaggcagccg gagcatatgg agcgccgggt ctccaatgca ggaggcccac
     1921 ctgctccccc agctgggggc cctcctccac ctccaggacc tccccctcct ccaggtcccc
     1981 ccccaccccc aggtctgccc tcctcagggg tatctggggc aggtcatgga gcaggggcag
     2041 ccccaccccc tgcaccccca ctccctacaa cacagggccc caatagtggg ggttccgggg
     2101 ccccaggcct ggctgctgcc attgctggag ccaaactcag gaaagtgagc aagcaggagg
     2161 aggcctctgg ggggcccctg gcccccaaag ctgagaacag tcgaagcact ggtggggggc
     2221 ttatggaaga gatgaacgcc atgctggccc ggagaagaaa agccacacag gttggggaga
     2281 agccccccaa agacgagtca gccagtgagg agtcagaggc ccgactccct gcccagagtg
     2341 aacctgtgag aagaccctgg gagaagaaca gcacaacctt gccaaggatg aagtcgtcct
     2401 cttctgtgac tacctccgag gcccacccct cgacgccctg ctccagtgat gactccgact
     2461 tggagagggt gaagcaggag cttctggaag aggtgcggaa ggagctacag aaaatgaaag
     2521 aggaaatcat cgaagtcttt gtccaggagc tgaggaagcg gggttctcct tgagcggccg
     2581 ccaccgcggt ggagctcggt accccgggtc gacctgcagc caagctt
//
