LOCUS       sequence-88840-e        2623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2623)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-VASP-N-10
FEATURES             Location/Qualifiers
     source          1..2623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1180..1857
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1906..2583
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gtcagatccg ctagcgatgc caccatgagc gagacggtca tctgttccag ccgggctact
       61 gtgatgcttt atgatgacag caacaagcga tggctccctg ctggcactgg tccccaggcc
      121 ttcagccgcg tccagatata ccacaacccc actgctaact ccttccgagt tgttggccgc
      181 aagatgcagc cggatcagca ggtggttatc aactgtgcca tcattcgggg tgtcaagtac
      241 aatcaggcca ctcccatctt ccatcagtgg cgagatgccc gccaggtctg gggcctcaac
      301 ttcggcagca aggaggacgc catacagttt gcaacaggca tggccaacgc cctagaggcc
      361 ttggaaggag gtgggcctcc cccagcccca gcaccccctg cctggtctgc ccagaatggt
      421 ccctccccag aggagctgga acaacagaaa aggcagccgg agcatatgga gcgccgggtc
      481 tccaatgcag gaggcccacc tgctccccca gctgggggcc ctcctccacc tccaggacct
      541 ccccctcctc caggtccccc cccaccccca ggtctgccct cctcaggggt atctggggca
      601 ggtcatggag caggggcagc cccaccccct gcacccccac tccctacaac acagggcccc
      661 aatagtgggg gttccggggc cccaggcctg gctgctgcca ttgctggagc caaactcagg
      721 aaagtgagca agcaggagga ggcctctggg gggcccctgg cccccaaagc tgagaacagt
      781 cgaagcactg gtggggggct tatggaagag atgaacgcca tgctggcccg gagaagaaaa
      841 gccacacagg ttggggagaa gccccccaaa gacgagtcag ccagtgagga gtcagaggcc
      901 cgactccctg cccagagtga acctgtgaga agaccctggg agaagaacag cacaaccttg
      961 ccaaggatga agtcgtcctc ttctgtgact acctccgagg cccacccctc gacgccctgc
     1021 tccagtgatg actccgactt ggagagggtg aagcaggagc ttctggaaga ggtgcggaag
     1081 gagctacaga aaatgaaaga ggaaatcatc gaagtctttg tccaggagct gaggaagcgg
     1141 ggttctccta gctccggagg ggatccaccg gtcgccacca tgagtgcgat taagccagac
     1201 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
     1261 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
     1321 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
     1381 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat
     1441 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata
     1501 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc
     1561 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
     1621 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc
     1681 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
     1741 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
     1801 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc
     1861 ggtagcggtc acggtactgg ttctactggt tctggttctt ctgagagtgc gattaagcca
     1921 gacatgaaga tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
     1981 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
     2041 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg
     2101 gtattcgccg aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg
     2161 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac
     2221 ataacaatgg aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc
     2281 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
     2341 tatgtgcgtg atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat
     2401 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
     2461 ccaggctacc actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac
     2521 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
     2581 taagcggccg cgactctaga tcataatcag ccataccaca ttt
//
