LOCUS       sequence-88841-e        3851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3851)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-VE-Cadherin-N-10
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2070..2081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2409..3086
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3135..3812
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gaccgtcaga tccgctagcc gccaccatgc agaggctcat gatgctcctc gccacatcgg
       61 gcgcctgcct gggcctgctg gcagtggcag cagtggcagc agcaggtgct aaccctgccc
      121 aacgggacac ccacagcctg ctgcccaccc accggcgcca aaagagagat tggatttgga
      181 accagatgca cattgatgaa gagaaaaaca cctcacttcc ccatcatgta ggcaagatca
      241 agtcaagcgt gagtcgcaag aatgccaagt acctgctcaa aggagaatat gtgggcaagg
      301 tcttccgggt cgatgcagag acaggagacg tgttcgccat tgagaggctg gaccgggaga
      361 atatctcaga gtaccacctc actgctgtca ttgtggacaa ggacactggt gaaaacctgg
      421 agactccttc cagcttcacc atcaaagttc atgacgtgaa cgacaactgg cctgtgttca
      481 cgcatcggtt gttcaatgcg tccgtgcctg agtcgtcggc tgtggggacc tcagtcatct
      541 ctgtgacagc agtggatgca gacgacccca ctgtgggaga ccacgcctct gtcatgtacc
      601 aaatcctgaa ggggaaagag tattttgcca tcgataattc tggacgtatt atcacaataa
      661 cgaaaagctt ggaccgagag aagcaggcca ggtatgagat cgtggtggaa gcgcgagatg
      721 cccagggcct ccggggggac tcgggcacgg ccaccgtgct ggtcactctg caagacatca
      781 atgacaactt ccccttcttc acccagacca agtacacatt tgtcgtgcct gaagacaccc
      841 gtgtgggcac ctctgtgggc tctctgtttg ttgaggaccc agatgagccc cagaaccgga
      901 tgaccaagta cagcatcttg cggggcgact accaggacgc tttcaccatt gagacaaacc
      961 ccgcccacaa cgagggcatc atcaagccca tgaagcctct ggattatgaa tacatccagc
     1021 aatacagctt catcgtcgag gccacagacc ccaccatcga cctccgatac atgagccctc
     1081 ccgcgggaaa cagagcccag gtcattatca acatcacaga tgtggacgag ccccccattt
     1141 tccagcagcc tttctaccac ttccagctga aggaaaacca gaagaagcct ctgattggca
     1201 cagtgctggc catggaccct gatgcggcta ggcatagcat tggatactcc atccgcagga
     1261 ccagtgacaa gggccagttc ttccgagtca caaaaaaggg ggacatttac aatgagaaag
     1321 aactggacag agaagtctac ccctggtata acctgactgt ggaggccaaa gaactggatt
     1381 ccactggaac ccccacagga aaagaatcca ttgtgcaagt ccacattgaa gttttggatg
     1441 agaatgacaa tgccccggag tttgccaagc cctaccagcc caaagtgtgt gagaacgctg
     1501 tccatggcca gctggtcctg cagatctccg caatagacaa ggacataaca ccacgaaacg
     1561 tgaagttcaa attcaccttg aatactgaga acaactttac cctcacggat aatcacgata
     1621 acacggccaa catcacagtc aagtatgggc agtttgaccg ggagcatacc aaggtccact
     1681 tcctacccgt ggtcatctca gacaatggga tgccaagtcg cacgggcacc agcacgctga
     1741 ccgtggccgt gtgcaagtgc aacgagcagg gcgagttcac cttctgcgag gatatggccg
     1801 cccaggtggg cgtgagcatc caggcagtgg tagccatctt actctgcatc ctcaccatca
     1861 cagtgatcac cctgctcatc ttcctgcggc ggcggctccg gaagcaggcc cgcgcgcacg
     1921 gcaagagcgt gccggagatc cacgagcagc tggtcaccta cgacgaggag ggcggcggcg
     1981 agatggacac caccagctac gatgtgtcgg tgctcaactc ggtgcgccgc ggcggggcca
     2041 agcccccgcg gcccgcgctg gacgcccggc cttccctcta tgcgcaggtg cagaagccac
     2101 cgaggcacgc gcctggggca cacggagggc ccggggagat ggcagccatg atcgaggtga
     2161 agaaggacga ggcggaccac gacggcgacg gcccccccta cgacacgctg cacatctacg
     2221 gctacgaggg ctccgagtcc atagccgagt ccctcagctc cctgggcacc gactcatccg
     2281 actctgacgt ggattacgac ttccttaacg actggggacc caggtttaag atgctggctg
     2341 agctgtacgg ctcggacccc cgggaggagc tgctgtattc gggaagcacg gatccaccgg
     2401 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa cctccgtatg gaaggcaacg
     2461 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
     2521 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
     2581 ccactgcatt ccattacggc aacagggtat tcgccgaata tccagaccac atacaagact
     2641 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
     2701 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
     2761 ttcgatttca cggtgtaaac tttcccgcca atggtccagt tatgcagaag aagacgctga
     2821 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc
     2881 gcatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
     2941 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
     3001 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
     3061 ctggattgcc tgacaatgcc agacgatccg gtagcggtca cggtactggt tctactggtt
     3121 ctggttcttc tgagagtgcg attaagccag acatgaagat caacctccgt atggaaggca
     3181 acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa
     3241 aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc
     3301 tgaccactgc attccattac ggcaacaggg tattcgccga atatccagac cacatacaag
     3361 actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag
     3421 acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata
     3481 aagttcgatt tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc
     3541 tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg actggtgata
     3601 ttcgcatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt
     3661 acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg
     3721 agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc
     3781 attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc
     3841 cataccacat t
//
