LOCUS       sequence-88843-e        2958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2958)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..2958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..723
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             772..1446
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acatgctagg tgacgtcaga tccgctagcg ctaccggtcg ccaccatgag tgcgattaag
       61 ccagacatga agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc
      121 gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa
      181 gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac
      241 agggtattcg ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag
      301 gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac
      361 gacataacaa tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt
      421 cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa
      481 atgtatgtgc gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga
      541 aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag
      601 ttaccaggct accactttgt ggaccactgc attgagattt taagccatga caaagattac
      661 aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga
      721 cgatccggta gcggtcacgg tactggttct actggttctg gttcttctga gagtgcgatt
      781 aagccagaca tgaagatcaa cctccgtatg gaaggcaacg taaacgggca ccactttgtg
      841 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc
      901 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc
      961 aacagggtat tcgccgaata tccagaccac atacaagact attttaagca gtcgtttcct
     1021 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg cattgccaga
     1081 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttca cggtgtaaac
     1141 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
     1201 aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc gcatggcttt gttgcttgaa
     1261 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
     1321 aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat
     1381 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
     1441 agacgatccg gactcagatc tggcagcggt ggaggcagcg catccggcgg aagcggaagc
     1501 tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg
     1561 agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta cagcctgggc
     1621 agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg cggcgtgtat
     1681 gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg gctcctgcag
     1741 gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa cacccgcacc
     1801 aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat cgacaaggtg
     1861 cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct caagggccaa
     1921 ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg ccggcaggtg
     1981 gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct ggccgaggac
     2041 atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga agccgaaaac
     2101 accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct tgaccttgaa
     2161 cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca cgaagaggaa
     2221 atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt ggatgtttcc
     2281 aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag tgtggctgcc
     2341 aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct ctctgaggct
     2401 gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga gtaccggaga
     2461 caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga gtccctggaa
     2521 cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta ccaagacact
     2581 attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg tcaccttcgt
     2641 gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc cacctacagg
     2701 aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt ttcctccctg
     2761 aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc aaaaaggaca
     2821 cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac ttctcagcat
     2881 cacgatgacc ttgaataagg atccaccgga tctagataac tgatcataat cagccatacc
     2941 acattgtaga gatttccc
//
