LOCUS       sequence-88845-e        4698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: tdEos-Vinculin-14
FEATURES             Location/Qualifiers
     source          1..4698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..696
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             742..1416
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gtcagatccg ctagcaccat ggtggcgatt aagccagaca tgaagatcaa cctccgtatg
       61 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
      121 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
      181 gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata tccagaccac
      241 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
      301 ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc
      361 tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt tatgcagaag
      421 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacc
      481 ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
      541 actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac
      601 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
      661 gttgctcatt ctggattgcc tgacaatgcc agacgatctc gaggtcacgg tactggttct
      721 actggttctg gttcttctga gagtgcgatt aagccagaca tgaagatcaa cctccgtatg
      781 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
      841 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
      901 gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata tccagaccac
      961 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
     1021 ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc
     1081 tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt tatgcagaag
     1141 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacc
     1201 ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
     1261 actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac
     1321 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
     1381 gttgctcatt ctggattgcc tgacaatgcc agacgagaga attcgggaaa ctcgagaaca
     1441 aagcttgaaa acggccgcat gccagtgttt catacgcgca cgatcgagag catcctggag
     1501 ccggtggcac agcagatctc ccacctggtg ataatgcacg aggagggcga ggtggacggc
     1561 aaagccattc ctgacctcac cgcgcccgtg gccgccgtgc aggcggccgt cagcaacctc
     1621 gtccgggttg gaaaagagac tgttcaaacc actgaggatc agattttgaa gagagatatg
     1681 ccaccagcat ttattaaggt tgagaatgct tgcaccaagc ttgtccaggc agctcagatg
     1741 cttcagtcag acccttactc agtgcctgct cgagattatc taattgatgg gtcaaggggc
     1801 atcctctctg gaacatcaga cctgctcctt accttcgatg aggctgaggt ccgtaaaatt
     1861 attagagttt gcaaaggaat tttggaatat cttacagtgg cagaggtggt ggagactatg
     1921 gaagatttgg tcacttacac aaagaatctt gggccaggaa tgactaagat ggccaagatg
     1981 attgacgaga gacagcagga gctcactcac caggagcacc gagtgatgtt ggtgaactcg
     2041 atgaacaccg tgaaagagtt gctgccagtt ctcatttcag ctatgaagat ttttgtaaca
     2101 actaaaaact caaaaaacca aggcatagag gaagctttaa aaaatcgcaa ttttactgta
     2161 gaaaaaatga gtgctgaaat taatgagata attcgtgtgt tacaactcac ctcttgggat
     2221 gaagatgcct gggccagcaa ggacactgaa gccatgaaga gagcattggc ctccatagac
     2281 tccaaactga accaggccaa aggttggctc cgtgacccta gtgcctcccc aggggatgct
     2341 ggtgagcagg ccatcagaca gatcttagat gaagctggaa aagttggtga actctgtgca
     2401 ggcaaagaac gcagggagat tctgggaact tgcaaaatgc tagggcagat gactgatcaa
     2461 gtggctgacc tccgtgccag aggacaagga tcctcaccgg tggccatgca gaaagctcag
     2521 caggtatctc agggtctgga tgtgctcaca gcaaaagtgg aaaatgcagc tcgcaagctg
     2581 gaagccatga ccaactcaaa gcagagcatt gcaaagaaga tcgatgctgc tcagaactgg
     2641 cttgcagatc caaatggtgg accggaagga gaagagcaga ttcgaggtgc tttggctgaa
     2701 gctcggaaaa tagcagaatt atgtgatgat cctaaagaaa gagatgacat tctacgttcc
     2761 cttggggaaa tatctgctct gacttctaaa ttagcagatc tacgaagaca ggggaaagga
     2821 gattctccag aggctcgagc cttggccaaa caggtggcca cggccctgca gaacctgcag
     2881 accaaaacca accgggctgt ggccaacagc agaccggcca aagcagctgt acaccttgag
     2941 ggcaagattg agcaagcaca gcggtggatt gataatccca cagtggatga ccgtggagtc
     3001 ggtcaggctg ccatccgggg gcttgtggcc gaagggcatc gtctggctaa tgttatgatg
     3061 gggccttatc ggcaagatct tctcgccaag tgtgaccgag tggaccagct gacagcccag
     3121 ctggctgacc tggctgccag aggggaaggg gagagtcctc aggcacgagc acttgcatct
     3181 cagctccaag actccttaaa ggatctaaaa gctcggatgc aggaggccat gactcaggaa
     3241 gtgtcagatg ttttcagcga taccacaact cccatcaagc tgttggcagt ggcagccacg
     3301 gcgcctcctg atgcgcctaa cagggaagag gtatttgatg agagggcagc taactttgaa
     3361 aaccattcag gaaagcttgg tgctacggcc gagaaggcgg ctgcggttgg tactgctaat
     3421 aaatcaacag tggaaggcat tcaggcctca gtgaagacgg cccgagaact cacaccccag
     3481 gtggtctcgg ctgctcgtat cttacttagg aaccctggaa atcaagctgc ttatgaacat
     3541 tttgagacca tgaagaacca gtggatcgat aatgttgaaa aaatgacagg gctggtggac
     3601 gaagccattg ataccaaatc tctgttggat gcttcagaag aagcaattaa aaaagacctg
     3661 gacaagtgca aggtagctat ggccaacatt cagcctcaga tgctggttgc tggggcaacc
     3721 agtattgctc gtcgggccaa ccggatcctg ctggtggcta agagggaggt ggagaattcc
     3781 gaggatccca agttccgtga ggctgtgaaa gctgcctctg atgaattgag caaaaccatc
     3841 tccccgatgg tgatggatgc aaaagctgtg gctggaaaca tttccgaccc tggactgcaa
     3901 aagagcttcc tggactcagg atatcggatc ctgggagctg tggccaaggt cagagaagcc
     3961 ttccaacctc aggagcctga cttcccgccg cctccaccag accttgaaca actccgacta
     4021 acagatgagc ttgctcctcc caaaccacct ctgcctgaag gtgaggtccc tccacctagg
     4081 cctccaccac cagaggaaaa ggatgaagag ttccctgagc agaaggccgg ggaggtgatt
     4141 aaccagccaa tgatgatggc tgccagacag ctccatgatg aagctcgcaa atggtccagc
     4201 aagggcaatg acatcattgc agcagccaag cgcatggctc tgctgatggc tgagatgtct
     4261 cggctggtaa gagggggcag tggtaccaag cgggcactca ttcagtgtgc caaggacatc
     4321 gccaaggcct cagatgaggt gactcggttg gccaaggagg ttgccaagca gtgcacagat
     4381 aaacggatta gaaccaacct cttacaggta tgtgagcgaa tcccaaccat aagcacccag
     4441 ctcaaaatcc tgtccacagt gaaggccacc atgctgggcc ggaccaacat cagtgatgag
     4501 gagtctgagc aggccacaga gatgctggtt cacaatgccc agaacctcat gcagtctgtg
     4561 aaggagactg tgcgggaagc tgaagctgct tcaatcaaaa ttcgaacaga tgctggattt
     4621 acactgcgct gggttagaaa gactccctgg taccagtagg cggccgcgac tctagatcat
     4681 aatcagccat accacatt
//
