LOCUS       sequence-88848-e        6725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6725)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..705
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             754..1428
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     RBS             3129..3137
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac
       61 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      121 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
      181 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat
      241 ccagaccaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
      301 agcttgactt tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg
      361 gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt
      421 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
      481 gtgctgactg gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt
      541 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt
      601 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
      661 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac
      721 ggtactggtt ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc
      781 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      841 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
      901 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
      961 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     1021 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     1081 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
     1141 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     1201 ggagtgctga ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga
     1261 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     1321 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     1381 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgatc cggaagtgct
     1441 gggagcgcaa gcggaagcgg aggctccggt gaggaaacag ctatatggga acaacataca
     1501 gtgacgcttc acagggctcc tggatttgga tttggaattg caatatctgg tggacgagat
     1561 aatcctcatt ttcagagtgg ggaaacgtca atagtgattt cagatgtgct gaaaggagga
     1621 ccagctgaag gacagctaca ggaaaatgac cgagttgcaa tggttaacgg agtttcaatg
     1681 gataatgttg aacatgcttt tgctgttcag caactaagga aaagtgggaa aaatgcaaaa
     1741 attacaatta gaaggaagaa gaaagttcaa ataccagtaa gtcgtcctga tcctgaacca
     1801 gtatctgata atgaagaaga tagttatgat gaggaaatac atgatccaag aagtggccgg
     1861 agtggtgtgg ttaacagaag gagtgagaag atttggccga gggatagaag tgcaagtaga
     1921 gagaggagct tgtccccgcg gtcagacagg cggtcagtgg cttccagcca gcctgctaaa
     1981 cctactaaag tcacactggt gaaatcccgg aaaaatgaag aatatggtct tcgattggca
     2041 agccatatat ttgttaagga aatttcacaa gatagtttgg cagcaagaga tggcaatatt
     2101 caagaaggtg atgttgtatt gaagataaat ggtactgtga cagaaaatat gtcattgaca
     2161 gatgcaaaga cattgataga aaggtctaaa ggcaaattaa aaatggtagt tcaaagagat
     2221 gaacgggcta cgctattgaa tgtccctgat ctttctgaca gcatccactc tgctaatgcc
     2281 tctgagagag acgacatttc agaaattcag tcactggcat cagatcattc tggtcgatca
     2341 cacgataggc ctccccgccg cagccggtca cgatctcctg accagcggtc agagccttct
     2401 gatcattcca ggcactcgcc gcagcagcca agcaatggca gtctgcggag tagagatgaa
     2461 gagagaattt ctaaacctgg ggctgtctca actcctgtaa agcatgctga tgatcacaca
     2521 cctaaaacag tggaagaagt tacagttgaa agaaatgaga aacaaacacc ttctcttcca
     2581 gaaccaaagc ctgtgtatgc ccaagttggc aaccagatgt ggatttacct gtcagtccat
     2641 ctgatggtgt cctacctaat tcaactcatg aagatgggat ttcttcggcc cagcatgaaa
     2701 ttggtaaaat tcagaaaagg agatagtgtg ggtttgcggc tggctggtgg aaatgatgtt
     2761 ggaatatttg tagctggcgt tctagaagat agccctgcag ccaaggaagg cttagaggaa
     2821 ggtgatcaaa ttctcagggt aaacaacgta gattttacaa atatcataag agaagaagcc
     2881 gtccttttcc tgcttgacct ccctaaagga gaagaagtga ccatattggc tcagaagaag
     2941 aaggatgttt atcgtcgcat tgtagaatca gatgtaggag attctttcta tattagaacc
     3001 cattttgaat atgaaaagga atctccctat ggacttagtt ttaacaaagg agaggtgttc
     3061 cgtgctgtgg ataccttgta caatggaaaa ctgggctctt ggcttgctat tcgaattggt
     3121 aaaaatcata aggaggtaga acgaggcatc atccctaata agaacagagc tgagcagctc
     3181 gccagtgtac agtatacact tccaaaaaca gcaggcggag accgtgctga cttctggaga
     3241 ttcagaggtc ttcgcagctc caagagaaat cttcgaaaaa gcagagagga tttgtccgct
     3301 cagcctgttc aaacaaagtt tccagcttat gaaagagtgg ttcttcgaga agctggattt
     3361 ctgaggcctg taaccatttt tggaccaata gctgatgttg ccagagaaaa gctggcaaga
     3421 gaagaaccag atatttatca aattgcaaag agtgaaccac gagacgctgg aactgaccaa
     3481 cgtagctctg gctatattcg cctgcataca ataaagcaaa tcatagatca agacaaacat
     3541 gctttattag atgtaacacc aaatgcagtt gatcgtctta actatgccca gtggtatcca
     3601 attgttgtat ttcttaaccc tgattctaag caaggagtaa aaacaatgag aatgaggtta
     3661 tgtccagaat ctcggaaaag tgccaggaag ttatacgagc gatctcataa acttgctaaa
     3721 aataatcacc atctttttac aactacaatt aacttaaatt caatgaatga tggttggtat
     3781 ggtgcgctga aagaagcagt tcaacaacag caaaaccagc tggtatgggt ttccgaggga
     3841 aaggcggatg gtgctacaag tgatgacctt gatttgcatg atgatcgtct gtcctacctg
     3901 tcagctccag gtagtgaata ctcaatgtat agcacggaca gtagacacac ttctgactat
     3961 gaagacacag acacagaagg cggggcctac actgatcaag aactagatga aactcttaat
     4021 gatgaggttg ggactccacc ggagtctgcc attacacggt cctctgagcc tgtaagagag
     4081 gactcctctg gaatgcatca tgaaaaccaa acatatcctc cttactcacc acaagcgcag
     4141 ccacaaccaa ttcatagaat agactcccct ggatttaagc cagcctctca acagaaagca
     4201 gaagcttcat ctccagtccc ttacctttcg cctgaaacaa acccagcatc atcaacctct
     4261 gctgttaatc ataatgtaaa tttaactaat gtcagactgg aggagcccac cccagctcct
     4321 tccacctctt actcaccaca agctgattct ttaagaacac caagtactga ggcagctcac
     4381 ataatgctaa gagatcaaga accatcattg tcgtcgcatg tagatccaac aaaggtgtat
     4441 agaaaggacc catatcccga ggaaatgatg aggcagaacc atgttttgaa acagccagcc
     4501 gttagtcacc cagggcacag gccagacaaa gagcctaatc tgacctatga accccaactc
     4561 ccatacgtag agaaacaagc cagcagagac ctggagcagc ccacatacag atacgagtcc
     4621 tcaagctata cggaccagtt ttctcgaaac tatgaacatc gtctgcgata cgaagatcgc
     4681 gtccccatgt atgaagaaca gtggtcatat tatgatgaca aacagcccta cccatctcgg
     4741 ccaccttttg ataatcagca ctctcaagac cttgactcca gacagcatcc cgaagagtcc
     4801 tcagaacgag ggtactttcc acgttttgaa gagccagccc ctctgtctta cgacagcaga
     4861 ccacgttacg aacaggcacc tagagcatcc gccctgcggc acgaagagca gccagctcct
     4921 gggtatgaca cacatggtag actcagaccg gaagcccagc cccacccttc agcagggccc
     4981 aagcctgcag agtccaagca gtattttgag caatattcac gcagttacga gcaagtacca
     5041 ccccaaggat ttacctctag agcaggtcat tttgagcctc tccatggtgc tgcagctgtc
     5101 cctccgctga taccttcatc tcagcataag ccagaagctc tgccttcaaa caccaaacca
     5161 ctgcctccac ccccaactca aaccgaagaa gaggaagatc cagcaatgaa gccacagtct
     5221 gtactcacca gagttaagat gtttgaaaac aaaaggtctg catccttaga gaccaagaag
     5281 gatgtaaatg acactggcag ttttaagcct ccagaagtag catctaaacc ttcaggtgct
     5341 cccatcattg gtcccaaacc cacttctcag aatcaattca gtgaacatga caaaactctg
     5401 tacaggattc cagaacctca aaaacctcaa ctgaagccac ctgaagatat tgttcggtcc
     5461 aatcattatg accctgaaga agatgaagaa tattatcgaa aacagctgtc atactttgac
     5521 cgaagaagtt ttgagaataa gcctcctgca cacattgctg ccagccatct ctccgagcct
     5581 gcaaagccag ctcattctca gaatcaatca aatttttcta gttattcttc aaagggaaag
     5641 cctcctgaag ctgatggtgt ggatagatca tttggcgaga aacgctatga acccatccag
     5701 gccactcccc ctcctcctcc attgccctcg cagtatgccc agccatctca gcctgtcacc
     5761 agcgcgtctc tccacataca ttctaaggga gcacatggtg aaggtaattc agtgtcattg
     5821 gattttcaga attccttagt gtccaaacca gacccacctc catctcagaa taagccagca
     5881 actttcagac caccaaaccg agaagatact gctcaggcag ctttctatcc ccagaaaagt
     5941 tttccagata aagccccagt taatggaact gaacagactc agaaaacagt cactccagca
     6001 tacaatcgat tcacaccaaa accatataca agttctgccc gaccatttga acgcaagttt
     6061 gaaagtccta aattcaatca caatcttctg ccaagtgaaa ctgcacataa acctgacttg
     6121 tcttcaaaaa ctcccacttc tccaaaaact cttgtgaaat cgcacagttt ggcacagcct
     6181 cctgagtttg acagtggagt tgaaactttc tctatccatg cagagaagcc taaatatcaa
     6241 ataaataata tcagcacagt gcctaaagct attcctgtga gtccttcagc tgtggaagag
     6301 gatgaagatg aagatggtca tactgtggtg gccacagccc gaggcatatt taacagcaat
     6361 gggggcgtgc tgagttccat agaaactggt gttagtataa ttatccctca aggagccatt
     6421 cccgaaggag ttgagcagga aatctatttc aaggtctgcc gggacaacag catccttcca
     6481 cctttagata aagagaaagg tgaaacactg ctgagtcctt tggtgatgtg tggtccccat
     6541 ggcctcaagt tcctgaagcc tgtggagctg cgcttaccac actgtgatcc taaaacctgg
     6601 caaaacaagt gtcttcccgg agatccaaat tatctcgttg gagcaaactg tgtttctgtc
     6661 cttattgacc acttttaagg atccaccgga tctagataac tgatcataat cagccatacc
     6721 acatt
//
