LOCUS       sequence-88850-e        3229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..3229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1784..2461
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2510..3187
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcaattcca tggcggcccc
       61 ccgcccgtct cccgcgatcc ccgtttcggt ctcggctccg gctttttacg ccccgcagaa
      121 gaagttcggc cctgtggtgg ccccaaagcc caaagtgaat cccttccggc ccagggacag
      181 cgagcctccc ccggcacccg gggcccagcg cgcacagatg ggccgggtgg gcgatattcc
      241 cccgccgccc ccggaagact ttcccctgcc tccacctccc cttgctgggg atggcgacga
      301 tgcagagggt gctctgggag gtgccttccc gccgccccct cccccgatcg aggaatcatt
      361 tccccctgcg cctctggagg aggagatctt cccttccccg ccgcctcctc cggaggagga
      421 gggagggcct gaggccccca taccgccccc accacagccc agggagaagg tgagcagtat
      481 tgatttggag atcgactctc tgtcctcact gctggatgac atgaccaaga atgatccttt
      541 caaagcccgg gtgtcatctg gatatgtgcc cccaccagtg gccactccat tcagttccaa
      601 gtccagtacc aagcctgcag ccgggggcac agcacccctg cctccttgga agtccccttc
      661 cagctcccag cctctgcccc aggttccggc tccggctcag agccagacac agttccatgt
      721 tcagccccag ccccagccca agcctcaggt ccaactccat gtccagtccc agacccagcc
      781 tgtgtctttg gctaacaccc agccccgagg gcccccagcc tcatctccgg ctccagcccc
      841 taagttttct ccagtgactc ctaagtttac tcctgtggct tccaagttca gtcctggagc
      901 cccaggtgga tctgggtcac aaccaaatca aaaattgggg caccccgaag ctctttctgc
      961 tggcacaggc tcccctcaac ctcccagctt cacctatgcc cagcagaggg agaagccccg
     1021 agtgcaggag aagcagcacc ccgtgccccc accggctcag aaccaaaacc aggtgcgctc
     1081 ccctggggcc ccagggcccc tgactctgaa ggaggtggag gagctggagc agctgaccca
     1141 gcagctaatg caggacatgg agcatcctca gaggcagaat gtggctgtca acgaactctg
     1201 cggccgatgc catcaacccc tggcccgggc gcagccagcc gtccgcgctc tagggcagct
     1261 gttccacatc gcctgcttca cctgccacca gtgtgcgcag cagctccagg gccagcagtt
     1321 ctacagtctg gagggggcgc cgtactgcga gggctgttac actgacaccc tggagaagtg
     1381 taacacctgc ggggagccca tcactgaccg catgctgagg gccacgggca aggcctatca
     1441 cccgcactgc ttcacctgtg tggtctgcgc ccgccccctg gagggcacct ccttcatcgt
     1501 ggaccaggcc aaccggcccc actgtgtccc cgactaccac aagcagtacg ccccgaggtg
     1561 ctccgtctgc tctgagccca tcatgcctga gcctggccga gatgagactg tgcgagtggt
     1621 cgccctggac aagaacttcc acatgaagtg ttacaagtgt gaggactgcg ggaagcccct
     1681 gtcgattgag gcagatgaca atggctgctt ccccctggac ggtcacgtgc tctgtcggaa
     1741 gtgccacact gctagagccc agacggatcc accggtcgcc accatgagtg cgattaagcc
     1801 agacatgaag atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
     1861 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
     1921 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
     1981 ggtattcgcc gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg
     2041 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga
     2101 cataacaatg gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc
     2161 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
     2221 gtatgtgcgt gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa
     2281 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
     2341 accaggctac cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
     2401 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
     2461 atccggtagc ggtcacggta ctggttctac tggttctggt tcttctgaga gtgcgattaa
     2521 gccagacatg aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat
     2581 cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa
     2641 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
     2701 cagggtattc gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa
     2761 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa
     2821 cgacataaca atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt
     2881 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
     2941 aatgtatgtg cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg
     3001 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     3061 gttaccaggc taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta
     3121 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     3181 acgataagcg gccgcgactc tagatcataa tcagccatac cacattgag
//
