LOCUS       sequence-88853-e        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: wtEos-H2B-6
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1157
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtga
      481 gtgcgattaa gccagacatg aagatcaacc tccgtatgga aggcaacgta aacgggcacc
      541 actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc
      601 ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc
      661 attacggcaa cagggtattc gccgaatatc cagaccacat acaagactat tttaagcagt
      721 cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca
      781 ttgccagaaa cgacataaca atggaagggg acactttcta taataaagtt cgatttcacg
      841 gtgtaaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct
      901 ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattacc atggctttgt
      961 tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga
     1021 agggtgtcaa gttaccaggc taccactttg tggaccactg cattgagatt ttaagccatg
     1081 acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg
     1141 acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac cacatt
//
