LOCUS       sequence-88856-e        1946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: Dendra2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..720
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atgaacaccc cgggaattaa cctgatcaag
       61 gaggacatgc gcgtgaaggt gcacatggag ggcaacgtga acggccacgc cttcgtgatc
      121 gagggcgagg gcaagggcaa gccctacgag ggcacccaga ccgccaacct gaccgtgaag
      181 gagggcgccc ccctgccctt cagctacgac atcctgacca ccgccgtgca ctacggcaac
      241 cgggtgttca ccaagtaccc cgaggacatc cccgactact tcaagcagag cttccccgag
      301 ggctacagct gggagcgcac catgaccttc gaggacaagg gcatctgcac catccgcagc
      361 gacatcagcc tggagggcga ctgcttcttc cagaacgtgc gcttcaaggg caccaacttc
      421 ccccccaacg gccccgtgat gcagaagaag accctgaagt gggagcccag caccgagaag
      481 ctgcacgtgc gcgacggcct gctggtgggc aacatcaaca tggccctgct gctggagggc
      541 ggcggccact acctgtgcga cttcaagacc acctacaagg ccaagaaggt ggtgcagctg
      601 cccgacgccc acttcgtgga ccaccgcatc gagatcctgg gcaacgacag cgactacaac
      661 aaggtgaagc tgtacgagca cgccgtggcc cgctacagcc ccctgcccag ccaggtgtgg
      721 tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgatgat
      781 gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg cttcgcgggc
      841 gacgatgccc cccgggccgt cttcccctcc atcgtggggc gccccaggca ccagggcgtg
      901 atggtgggca tgggtcagaa ggattcctat gtgggcgacg aggcccagag caagagaggc
      961 atcctcaccc tgaagtaccc catcgagcac ggcatcgtca ccaactggga cgacatggag
     1021 aaaatctggc accacacctt ctacaatgag ctgcgtgtgg ctcccgagga gcaccccgtg
     1081 ctgctgaccg aggcccccct gaaccccaag gccaaccgcg agaagatgac ccagatcatg
     1141 tttgagacct tcaacacccc agccatgtac gttgctatcc aggctgtgct atccctgtac
     1201 gcctctggcc gtaccactgg catcgtgatg gactccggtg acggggtcac ccacactgtg
     1261 cccatctacg aggggtatgc cctcccccat gccatcctgc gtctggacct ggctggccgg
     1321 gacctgactg actacctcat gaagatcctc accgagcgcg gctacagctt caccaccacg
     1381 gccgagcggg aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc cctggacttc
     1441 gagcaagaga tggccacggc tgcttccagc tcctccctgg agaagagcta cgagctgcct
     1501 gacggccagg tcatcaccat tggcaatgag cggttccgct gccctgaggc actcttccag
     1561 ccttccttcc tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa ctccatcatg
     1621 aagtgtgacg tggacatccg caaagacctg tacgccaaca cagtgctgtc tggcggcacc
     1681 accatgtacc ctggcattgc cgacaggatg cagaaggaga tcactgccct ggcacccagc
     1741 acaatgaaga tcaagatcat tgctcctcct gagcgcaagt actccgtgtg gatcggcggc
     1801 tccatcctgg cctcgctgtc caccttccag cagatgtgga tcagcaagca ggagtatgac
     1861 gagtccggcc cctccatcgt ccaccgcaaa tgcttctagg gatccaccgg atctagataa
     1921 ctgatcataa tcagccatac cacatt
//
