LOCUS       sequence-88857-e        3533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 490 / 553, 
            Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3533)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..723
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     protein_bind    2542..2553
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgaaca ccccgggaat taacctgatc
       61 aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg tgaacggcca cgccttcgtg
      121 atcgagggcg agggcaaggg caagccctac gagggcaccc agaccgccaa cctgaccgtg
      181 aaggagggcg cccccctgcc cttcagctac gacatcctga ccaccgccgt gcactacggc
      241 aaccgggtgt tcaccaagta ccccgaggac atccccgact acttcaagca gagcttcccc
      301 gagggctaca gctgggagcg caccatgacc ttcgaggaca agggcatctg caccatccgc
      361 agcgacatca gcctggaggg cgactgcttc ttccagaacg tgcgcttcaa gggcaccaac
      421 ttccccccca acggccccgt gatgcagaag aagaccctga agtgggagcc cagcaccgag
      481 aagctgcacg tgcgcgacgg cctgctggtg ggcaacatca acatggccct gctgctggag
      541 ggcggcggcc actacctgtg cgacttcaag accacctaca aggccaagaa ggtggtgcag
      601 ctgcccgacg cccacttcgt ggaccaccgc atcgagatcc tgggcaacga cagcgactac
      661 aacaaggtga agctgtacga gcacgccgtg gcccgctaca gccccctgcc cagccaggtg
      721 tggtccggac tcagatctgg cagcggtgga ggcttggagg agccggaggc cgcgacgcgg
      781 acagccgcgg ccgtggattg caaagacaga cccgggttcc cagtgaagag gttaatacaa
      841 gcccgcttgc ccttcaaacg cctgaacctt gttcccaagg agaaagttga ggaagacaca
      901 tcaccaaaag cagctgtgga aagcaaagtc ccggatctac aattgtcttt gggcactttt
      961 gaaagccagt gtcacacagg ctctcacgta ggcttgagta caaagttggt cggtggccag
     1021 ggccccattg atagcttctt gagggcaaca atcaagccag taccgagtgt ggtcattatc
     1081 gacctgacgg agaactgcag tgacattcca gacagccctg agggccacag tgaactaagt
     1141 cctgacactg ctggtgtggt caccacagtg gaaggagctg ccaagcagca ggagcactct
     1201 gcagcagagc tgtgtctcct agagacccct tcagacatca cttgtcacat ggaagaggag
     1261 cctgggagtc caggagaccc aaagaggacg ggtgactgtc aggctggttc actacagagc
     1321 tgtccggagc tgaccccagg ttctaggaca tgccccacca aggagttatc aagctggagc
     1381 aaggcagggg atctcctatt tatagagaaa gtgcctgtgg tggtgctaga ggacatcctg
     1441 gccactaagc cctccattgc ctctcttccc atgatgtccc tggataggag tgtcacttca
     1501 gagagtgaaa tactggagtc ctgccccgag gatgactcca tactgagcca ctcctccacc
     1561 aactcctctt ctccaaccag ctctcctgag ggaccatcca cacccccaga acatcggggt
     1621 gggagaagct ccccctccac cccagcctgc agagttgcca agaactttgt caaaggctcc
     1681 acagagaagg gcaggagcaa actgcacaga gatagagaac agcagaggga agagaaggaa
     1741 aagctgaggg aagagatacg acgagccaag gaggaggcac ggaagaagaa ggaggaggag
     1801 aaggagctga aggaaaagga gcggagggaa aagcgggaga aagatgagaa ggagaaggcg
     1861 gagaagcagc ggctaaagga ggaaaaacgc aaggagcggc aggaagctct ggaggctaaa
     1921 ctggaggaga agaggaagaa ggaggaagag aagcggttac gggaggaaga gaagcggtta
     1981 cgggaagaag agaagcgcat taaggcagaa aaggcagaga tcactcgatt cttccagaaa
     2041 ccgaagactc cgcaggctcc caagaccctg gccggttcct gtggtaagtt tgcccccttt
     2101 gaaatcaaag aacacatggt gctggctcca cggtgccgag ctgccttgga tcaagacctg
     2161 tgcgatcagc tggaccagct cctgcagcag cagagtgtgg caagcacctt cctcagtgac
     2221 ctaaaaagcc ggctgccctt gagatcgggg cccacccggg tctgtggcca tgacacagac
     2281 atcatgaaca gggatgtggt cattgtggag agcagcaagg tggacggtgt gtctgaaagg
     2341 aagaagtttg gccgcatgaa gcttctgcag ttctcagaga accaccggcc agcatactgg
     2401 gggacgtgga acaagaagac agccatcatc cgcccccgga acccctgggc ccaggacaaa
     2461 gaccttcttg actatgaggt agacagtgac gatgagtggg aagaggagga acctggggag
     2521 tctttgtccc acagtgaggg ggatgaggat gacgatgtgg gggaagatga ggatgaggac
     2581 gatggcttct ttgtgcccca tgggtactta tctgaagatg aaggcgtgac tgaggagtgt
     2641 gcagaccctg agaaccacaa agtacaccag aagctgaagg ccaaggagtg ggatgagctc
     2701 ctagccaagg gcaagcgctt tcgtgtgctg cagcctgtgc acgtgggatg tgtatgggca
     2761 tcagaggcag ccaactgcac gagttccgac ttgaagctgc tgcagcagtt cactgcatgc
     2821 ctgctggatg tggcatcccc tgatgagcca gaacccgggg cctccaggag ggaaaagagg
     2881 gaccagcata tcctggccca gttgcttccg ctgctacatg gcaatgtgaa tgggagcaag
     2941 gtgatcatcc atgaattcca ggagcaatgc cgcaggggcc tgctgaccct tccaagtccc
     3001 accccacacc tgcagatgcc caacttggag gatgctgttg ctgtgccatc caaggccagg
     3061 ctgaagcgcc ttatctctga gaactctgcc tatgagaagc ggcccaactt ccgtatgtgc
     3121 tggtatgtgc acccagaggt gctcaagagc tttggccagg agtgcctgcc agtgccctgc
     3181 cagtggacct acatcaccac catgccctca gcacccaggg aagacagtgg cagtgcttct
     3241 actgaggggc ctggccagag cacacctatg ctactcaagc ggaagccagc ggccaccatg
     3301 tgcatcacgc agtttatgaa gaagcggcgc tatgatggac aggttgggtc tggggacatg
     3361 gatggcttcc aggcagacac agaagaggat gaagaggatg acacagactg tatgatcata
     3421 gatgtgccag atgttgggag tgacgtgtca gaagctccca tacctgctcc cacactttgc
     3481 aagtgaggat ccaccggatc tagataactg atcataatca gccataccac att
//
