LOCUS       sequence-88863-e        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     CDS             596..1288
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gacgtcagat ccgctagcca ccatgggccc caagcggcgg cagctgacgt tccgggagaa
       61 gtcgcggatc atccaggagg tggaggagaa cccggaccta cgcaagggcg agatcgcgcg
      121 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      181 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      241 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      301 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcgc atagccgagg agctgggcat
      361 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      421 gtcctgcagc ggcgtggccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      481 gcctgccagt ccggccgcgg tgccctcgga gggcagtggc gggagcactc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgaa
      601 caccccggga attaacctga tcaaggagga catgcgcgtg aaggtgcaca tggagggcaa
      661 cgtgaacggc cacgccttcg tgatcgaggg cgagggcaag ggcaagccct acgagggcac
      721 ccagaccgcc aacctgaccg tgaaggaggg cgcccccctg cccttcagct acgacatcct
      781 gaccaccgcc gtgcactacg gcaaccgggt gttcaccaag taccccgagg acatccccga
      841 ctacttcaag cagagcttcc ccgagggcta cagctgggag cgcaccatga ccttcgagga
      901 caagggcatc tgcaccatcc gcagcgacat cagcctggag ggcgactgct tcttccagaa
      961 cgtgcgcttc aagggcacca acttcccccc caacggcccc gtgatgcaga agaagaccct
     1021 gaagtgggag cccagcaccg agaagctgca cgtgcgcgac ggcctgctgg tgggcaacat
     1081 caacatggcc ctgctgctgg agggcggcgg ccactacctg tgcgacttca agaccaccta
     1141 caaggccaag aaggtggtgc agctgcccga cgcccacttc gtggaccacc gcatcgagat
     1201 cctgggcaac gacagcgact acaacaaggt gaagctgtac gagcacgccg tggcccgcta
     1261 cagccccctg cccagccagg tgtggtaagc ggccgcgact ctagatcata atcagccata
     1321 cc
//
