LOCUS       sequence-88869-e        1653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 490 / 553, 
            Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: Dendra2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..1614
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ccgtagcgta ccggatcagt ctcgagctca agcttcaatt ctgcatcgac tgccaccatg
       61 gccgtcaatg tgtactccac atctgtgacc agtgaaaatc tgagtcgcca tgatatgctt
      121 gcatgggtca acgactccct gcacctcaac tataccaaga tagaacagct ttgttcaggg
      181 gcagcctact gccagttcat ggacatgctc ttccccggct gtgtgcactt gaggaaagtg
      241 aagttccagg ccaaactaga gcatgaatac atccacaact tcaaggtgct gcaagcagct
      301 ttcaagaaga tgggtgttga caaaatcatt cctgtagaga aattagtgaa aggaaaattc
      361 caagataatt ttgagtttat tcagtggttt aagaaattct ttgacgcaaa ctatgatgga
      421 aaggattaca accctctgct ggcgcggcag ggccaggacg tagcgccacc tcctaaccca
      481 ggtgatcaga tcttcaacaa atccaagaaa ctcattggca cagcagttcc acagaggacg
      541 tcccccacag gcccaaaaaa catgcagacc tctggccggc tgagcaatgt ggcccccccc
      601 tgcattctcc ggaagaatcc tccatcagcc cgaaatggcg gccatgagac tgatgcccaa
      661 attcttgaac tcaaccaaca gctggtggac ttgaagctga cagtggatgg gctggagaag
      721 gaacgtgact tctacttcag caaacttcgt gacatcgagc tcatctgcca ggagcatgaa
      781 agtgaaaaca gccctgttat ctcaggcatc attggcatcc tctatgccac agaggaagga
      841 ttcgcacccc ctgaggacga tgagattgaa gagcatcaac aagaagacca ggacgagtac
      901 ctggatccac cggtcgccac catgaacacc ccgggaatta acctgatcaa ggaggacatg
      961 cgcgtgaagg tgcacatgga gggcaacgtg aacggccacg ccttcgtgat cgagggcgag
     1021 ggcaagggca agccctacga gggcacccag accgccaacc tgaccgtgaa ggagggcgcc
     1081 cccctgccct tcagctacga catcctgacc accgccgtgc actacggcaa ccgggtgttc
     1141 accaagtacc ccgaggacat ccccgactac ttcaagcaga gcttccccga gggctacagc
     1201 tgggagcgca ccatgacctt cgaggacaag ggcatctgca ccatccgcag cgacatcagc
     1261 ctggagggcg actgcttctt ccagaacgtg cgcttcaagg gcaccaactt cccccccaac
     1321 ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa gctgcacgtg
     1381 cgcgacggcc tgctggtggg caacatcaac atggccctgc tgctggaggg cggcggccac
     1441 tacctgtgcg acttcaagac cacctacaag gccaagaagg tggtgcagct gcccgacgcc
     1501 cacttcgtgg accaccgcat cgagatcctg ggcaacgaca gcgactacaa caaggtgaag
     1561 ctgtacgagc acgccgtggc ccgctacagc cccctgccca gccaggtgtg gtaagcggcc
     1621 gcgactctag atcataatca gccataccac att
//
