LOCUS       sequence-88870-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 490 / 553, 
            Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..53
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     4..24
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             154..843
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
       61 tgaaccgtca gatccgctag cgccaccatg ctgctatccg tgccgttgct gctcggcctc
      121 ctcggcctgg ccgtcgccgc accggtcgcc accatgaaca ccccgggaat taacctgatc
      181 aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg tgaacggcca cgccttcgtg
      241 atcgagggcg agggcaaggg caagccctac gagggcaccc agaccgccaa cctgaccgtg
      301 aaggagggcg cccccctgcc cttcagctac gacatcctga ccaccgccgt gcactacggc
      361 aaccgggtgt tcaccaagta ccccgaggac atccccgact acttcaagca gagcttcccc
      421 gagggctaca gctgggagcg caccatgacc ttcgaggaca agggcatctg caccatccgc
      481 agcgacatca gcctggaggg cgactgcttc ttccagaacg tgcgcttcaa gggcaccaac
      541 ttccccccca acggccccgt gatgcagaag aagaccctga agtgggagcc cagcaccgag
      601 aagctgcacg tgcgcgacgg cctgctggtg ggcaacatca acatggccct gctgctggag
      661 ggcggcggcc actacctgtg cgacttcaag accacctaca aggccaagaa ggtggtgcag
      721 ctgcccgacg cccacttcgt ggaccaccgc atcgagatcc tgggcaacga cagcgactac
      781 aacaaggtga agctgtacga gcacgccgtg gcccgctaca gccccctgcc cagccaggtg
      841 tggtccggaa aggacgagct gtaagaattc tgcagtcgac ggtaccgcgg gcccgggatc
      901 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
      961 tttaaaaaaa cctcccacac ctccccctga acct
//
