LOCUS       sequence-88874-e        2234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 490 / 553, 
            Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2234)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..2234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1503..2195
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 ggacggtcag atccgctagc gccaccatgg gctgtgtgca atgtaaggat aaagaagcaa
       61 caaaactgac ggaggagagg gacggcagcc tgaaccagag ctctgggtac cgctatggca
      121 cagaccccac ccctcagcac taccccagct tcggtgtgac ctccatcccc aactacaaca
      181 acttccacgc agccgggggc caaggactca ccgtctttgg aggtgtgaac tcttcgtctc
      241 atacggggac cttgcgtacg agaggaggaa caggagtgac actctttgtg gccctttatg
      301 actatgaagc acggacagaa gatgacctga gttttcacaa aggagaaaaa tttcaaatat
      361 tgaacagctc ggaaggagat tggtgggaag cccgctcctt gacaactgga gagacaggtt
      421 acattcccag caattatgtg gctccagttg actctatcca ggcagaagag tggtactttg
      481 gaaaacttgg ccgaaaagat gctgagcgac agctattgtc ctttggaaac ccaagaggta
      541 cctttcttat ccgcgagagt gaaaccacca aaggtgccta ttcactttct atccgtgatt
      601 gggatgatat gaaaggagac catgtcaaac attataaaat tcgcaaactt gacaatggtg
      661 gatactacat taccacccgg gcccagtttg aaacacttca gcagcttgta caacattact
      721 caggtacctg gaatggaaac acaaaagtag ccataaagac tcttaaacca ggcacaatgt
      781 cccccgaatc attccttgag gaagcgcaga tcatgaagaa gctgaagcac gacaagctgg
      841 tccagctcta tgcagtggtg tctgaggagc ccatctacat cgtcaccgag tatatgaaca
      901 aaggaagttt actggatttc ttaaaagatg gagaaggaag agctctgaaa ttaccaaatc
      961 ttgtggacat ggcagcacag gtggctgcag gaatggctta catcgagcgc atgaattata
     1021 tccatagaga tctgcgatca gcaaacattc tagtggggaa tggactcata tgcaagattg
     1081 ctgacttcgg attggcccga ttgatagaag acaatgagta cacagcaaga caaggtgcaa
     1141 agttccccat caagtggacg gcccccgagg cagccctgta cgggaggttc acaatcaagt
     1201 ctgacgtgtg gtcttttgga atcttactca cagagctggt caccaaagga agagtgccat
     1261 acccaggcat gaacaaccgg gaggtgctgg agcaggtgga gcgaggctac aggatgccct
     1321 gcccgcagga ctgccccatc tctctgcatg agctcatgat ccactgctgg aaaaaggacc
     1381 ctgaagaacg ccccactttt gagtacttgc agagcttcct ggaagactac tttaccgcga
     1441 cagagcccca gtaccaacct ggtgaaaacc tggcatccag cggggatcca ccggtcgcca
     1501 ccatgaacac cccgggaatt aacctgatca aggaggacat gcgcgtgaag gtgcacatgg
     1561 agggcaacgt gaacggccac gccttcgtga tcgagggcga gggcaagggc aagccctacg
     1621 agggcaccca gaccgccaac ctgaccgtga aggagggcgc ccccctgccc ttcagctacg
     1681 acatcctgac caccgccgtg cactacggca accgggtgtt caccaagtac cccgaggaca
     1741 tccccgacta cttcaagcag agcttccccg agggctacag ctgggagcgc accatgacct
     1801 tcgaggacaa gggcatctgc accatccgca gcgacatcag cctggagggc gactgcttct
     1861 tccagaacgt gcgcttcaag ggcaccaact tcccccccaa cggccccgtg atgcagaaga
     1921 agaccctgaa gtgggagccc agcaccgaga agctgcacgt gcgcgacggc ctgctggtgg
     1981 gcaacatcaa catggccctg ctgctggagg gcggcggcca ctacctgtgc gacttcaaga
     2041 ccacctacaa ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg gaccaccgca
     2101 tcgagatcct gggcaacgac agcgactaca acaaggtgaa gctgtacgag cacgccgtgg
     2161 cccgctacag ccccctgccc agccaggtgt ggtaagcggc cgcgactcta gatcataatc
     2221 agccatacca catt
//
