LOCUS       sequence-88878-e        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus Histones, Excitation: 490 / 553, Emission: 507
            / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             474..1166
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatgaaca
      481 ccccgggaat taacctgatc aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg
      541 tgaacggcca cgccttcgtg atcgagggcg agggcaaggg caagccctac gagggcaccc
      601 agaccgccaa cctgaccgtg aaggagggcg cccccctgcc cttcagctac gacatcctga
      661 ccaccgccgt gcactacggc aaccgggtgt tcaccaagta ccccgaggac atccccgact
      721 acttcaagca gagcttcccc gagggctaca gctgggagcg caccatgacc ttcgaggaca
      781 agggcatctg caccatccgc agcgacatca gcctggaggg cgactgcttc ttccagaacg
      841 tgcgcttcaa gggcaccaac ttccccccca acggccccgt gatgcagaag aagaccctga
      901 agtgggagcc cagcaccgag aagctgcacg tgcgcgacgg cctgctggtg ggcaacatca
      961 acatggccct gctgctggag ggcggcggcc actacctgtg cgacttcaag accacctaca
     1021 aggccaagaa ggtggtgcag ctgcccgacg cccacttcgt ggaccaccgc atcgagatcc
     1081 tgggcaacga cagcgactac aacaaggtga agctgtacga gcacgccgtg gcccgctaca
     1141 gccccctgcc cagccaggtg tggtaagcgg ccgcgactct agatcataat cagccatacc
     1201 acatt
//
