LOCUS       sequence-88886-e        1844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1844)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..1844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1113..1805
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggatc tgcaagcctc gttgctgtcc actggcccca
       61 atgccagcaa catctccgat ggccaggata atttcacatt ggcggggcca cctcctcgca
      121 caaggagtgt ctcctacatc aacatcatca tgccttcagt gtttggtacc atctgtctcc
      181 tgggcattgt gggaaactcc acagtcattt ttgccgtggt gaagaaatcc aagctgcact
      241 ggtgcagcaa cgtccctgac atcttcatca tcaacctctc tgtggtggat ctgcttttcc
      301 tgctgggcat gcctttcatg atccaccagc tcatgggtaa tggtgtctgg cactttgggg
      361 aaaccatgtg caccctcatc acagccatgg acgccaacag tcagttcacc agcacctaca
      421 tcctgactgc tatggccatt gaccgctact tggccaccgt ccatcccatc tcctccacca
      481 agttccggaa gccctccatg gccaccctgg tgatctgcct cctgtgggct ctctcgttca
      541 ttagcatcac tcctgtgtgg ctctatgcca ggcttatccc cttcccaggg ggtgctgtgg
      601 gctgtggcat ccgcctacca aacccagata ctgatcttta ctggttcact ctgtatcagt
      661 ttttcctggc cttcgccctt ccgtttgtgg tcatcactgc tgcgtacgtg aaaatactac
      721 agcgcatgac gtcttcggtg gccccagcct ctcaacgcag catccggctt cggacaaaga
      781 gggtgacccg cacagccatt gccatctgtc tggtcttctt tgtgtgctgg gcgccctact
      841 acgtgctgca gctgacccag ttgtccatca gccgcccgac cctcacattc gtctacctgt
      901 acaatgcggc tatcagcttg ggctatgcca acagctgcct caatcccttt gtgtacatag
      961 tactctgtga gacctttcga aaacgcttgg tgctgtcggt gaagcccgcg gcccaggggc
     1021 agcttcgcac ggtcagcaat gctcagacag ctgacgagga gaggacagaa agcaaaggca
     1081 ccggaggtag cggggatcca ccggtcgcca ccatgaacac cccgggaatt aacctgatca
     1141 aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt gaacggccac gccttcgtga
     1201 tcgagggcga gggcaagggc aagccctacg agggcaccca gaccgccaac ctgaccgtga
     1261 aggagggcgc ccccctgccc ttcagctacg acatcctgac caccgccgtg cactacggca
     1321 accgggtgtt caccaagtac cccgaggaca tccccgacta cttcaagcag agcttccccg
     1381 agggctacag ctgggagcgc accatgacct tcgaggacaa gggcatctgc accatccgca
     1441 gcgacatcag cctggagggc gactgcttct tccagaacgt gcgcttcaag ggcaccaact
     1501 tcccccccaa cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga
     1561 agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa catggccctg ctgctggagg
     1621 gcggcggcca ctacctgtgc gacttcaaga ccacctacaa ggccaagaag gtggtgcagc
     1681 tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct gggcaacgac agcgactaca
     1741 acaaggtgaa gctgtacgag cacgccgtgg cccgctacag ccccctgccc agccaggtgt
     1801 ggtaagcggc cgcgactcta gatcataatc agccatacca catt
//
