LOCUS       sequence-88889-e        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             110..799
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtaccg gtcgccacca tgaacacccc
      121 gggaattaac ctgatcaagg aggacatgcg cgtgaaggtg cacatggagg gcaacgtgaa
      181 cggccacgcc ttcgtgatcg agggcgaggg caagggcaag ccctacgagg gcacccagac
      241 cgccaacctg accgtgaagg agggcgcccc cctgcccttc agctacgaca tcctgaccac
      301 cgccgtgcac tacggcaacc gggtgttcac caagtacccc gaggacatcc ccgactactt
      361 caagcagagc ttccccgagg gctacagctg ggagcgcacc atgaccttcg aggacaaggg
      421 catctgcacc atccgcagcg acatcagcct ggagggcgac tgcttcttcc agaacgtgcg
      481 cttcaagggc accaacttcc cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg
      541 ggagcccagc accgagaagc tgcacgtgcg cgacggcctg ctggtgggca acatcaacat
      601 ggccctgctg ctggagggcg gcggccacta cctgtgcgac ttcaagacca cctacaaggc
      661 caagaaggtg gtgcagctgc ccgacgccca cttcgtggac caccgcatcg agatcctggg
      721 caacgacagc gactacaaca aggtgaagct gtacgagcac gccgtggccc gctacagccc
      781 cctgcccagc caggtgtggt ccggagaagg gcaagggcaa gggcaagggc cgggccgcgg
      841 ctacgcgtat cgatccatgt tcgaggcgcg cctggtccag ggctccatcc tcaagaaggt
      901 gttggaggca ctcaaggacc tcatcaacga ggcctgctgg gatattagct ccagcggtgt
      961 aaacctgcag agcatggact cgtcccacgt ctctttggtg cagctcaccc tgcggtctga
     1021 gggcttcgac acctaccgct gcgaccgcaa cctggccatg ggcgtgaacc tcaccagtat
     1081 gtccaaaata ctaaaatgcg ccggcaatga agatatcatt acactaaggg ccgaagataa
     1141 cgcggatacc ttggcgctag tatttgaagc accaaaccag gagaaagttt cagactatga
     1201 aatgaagttg atggatttag atgttgaaca acttggaatt ccagaacagg agtacagctg
     1261 tgtagtaaag atgccttctg gtgaatttgc acgtatatgc cgagatctca gccatattgg
     1321 agatgctgtt gtaatttcct gtgcaaaaga cggagtgaaa ttttctgcaa gtggagaact
     1381 tggaaatgga aacattaaat tgtcacagac aagtaatgtc gataaagagg aggaagctgt
     1441 taccatagag atgaatgaac cagttcaact aacttttgca ctgaggtacc tgaacttctt
     1501 tacaaaagcc actccactct cttcaacggt gacactcagt atgtctgcag atgtacccct
     1561 tgttgtagag tataaaattg cggatatggg acacttaaaa tactacttgg ctcccaagat
     1621 cgaggatgaa gaaggatctt agggatccac cggatctaga taactgatca taatcagcca
     1681 taccacatt
//
