LOCUS       sequence-88891-e        2754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centromere, Excitation: 490 / 553, Emission: 507 / 
            573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2754)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-Rad21-C-18
FEATURES             Location/Qualifiers
     source          1..2754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..734
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gcctgctagt gacgtcagat ccgctagcgc taccggtcgc caccatgaac accccgggaa
       61 ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc
      121 acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc cagaccgcca
      181 acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg accaccgccg
      241 tgcactacgg caaccgggtg ttcaccaagt accccgagga catccccgac tacttcaagc
      301 agagcttccc cgagggctac agctgggagc gcaccatgac cttcgaggac aagggcatct
      361 gcaccatccg cagcgacatc agcctggagg gcgactgctt cttccagaac gtgcgcttca
      421 agggcaccaa cttccccccc aacggccccg tgatgcagaa gaagaccctg aagtgggagc
      481 ccagcaccga gaagctgcac gtgcgcgacg gcctgctggt gggcaacatc aacatggccc
      541 tgctgctgga gggcggcggc cactacctgt gcgacttcaa gaccacctac aaggccaaga
      601 aggtggtgca gctgcccgac gcccacttcg tggaccaccg catcgagatc ctgggcaacg
      661 acagcgacta caacaaggtg aagctgtacg agcacgccgt ggcccgctac agccccctgc
      721 ccagccaggt gtggtccgga ctcagatctc gaggaagcgg cggatctggg tccggcagct
      781 ccggcagctt ctacgcacat tttgttctca gtaaaagagg gcctctggcc aaaatttggc
      841 tagcggccca ttgggataag aagctaacca aagcccatgt gttcgagtgt aatttagaga
      901 gcagcgtgga gagtatcatc tcaccaaagg tgaaaatggc attacggaca tcaggacatc
      961 tcttactggg agtagttcga atctatcaca ggaaagccaa ataccttctt gcagactgta
     1021 atgaagcatt cattaagata aagatggctt ttcggccagg tgtggttgac ctgcctgagg
     1081 aaaatcggga agcagcttat aatgccatta ctttacctga agaatttcat gactttgatc
     1141 agccactgcc tgacttagat gacatcgatg tggcccagca gttcagcttg aatcagagta
     1201 gagtggaaga gataaccatg agagaagaag ttgggaacat cagtatttta caagaaaatg
     1261 attttggtga ttttggaatg gatgatcgtg agataatgag agaaggcagt gcttttgagg
     1321 atgacgacat gttagtaagc actactactt ctaacctcct attagagtct gaacagagca
     1381 ccagcaatct gaatgagaaa attaaccatt tagaatatga agatcaatat aaggatgata
     1441 attttggaga aggaaatgat ggtggaatat tagatgacaa acttattagt aataatgatg
     1501 gcggtatctt tgatgatccc cctgccctct ctgaggcagg ggtgatgttg ccagagcagc
     1561 ctgcacatga cgatatggat gaggatgata atgtatcaat gggtgggcct gatagtcctg
     1621 attcagtgga tcccgttgaa ccaatgccaa ccatgactga tcaaacaaca cttgttccaa
     1681 atgaggaaga agcatttgca ttggagccta ttgatataac tgttaaagaa acaaaagcca
     1741 agaggaagag gaagctaatt gttgacagtg tcaaagagtt ggatagcaag acaattagag
     1801 cccaacttag tgattattca gatattgtta ctactttgga tctggcaccg cccaccaaga
     1861 aattgatgat gtggaaagag acaggaggag tagaaaaact gttttcttta cctgctcagc
     1921 ctttgtggaa taacagacta ctgaagctct ttacacgctg tcttacaccg cttgtaccag
     1981 aagaccttag aaaaaggagg aaaggaggag aggcagataa tttggatgaa ttcctcaaag
     2041 aatttgaaaa tccagaggtt cctagagagg accagcaaca gcagcatcag cagcgtgatg
     2101 ttatcgatga gcccattatt gaagagccaa gccgcctcca ggagtcagtg atggaggcca
     2161 gcagaacaaa catagatgag tcagctatgc ctccaccacc acctcaggga gttaagcgaa
     2221 aagctggaca aattgaccca gagcctgtga tgcctcctca gcaggtagag cagatggaaa
     2281 taccacctgt agagcttccc ccagaagaac ctccaaatat ctgtcagcta ataccagagt
     2341 tagaacttct gccagaaaaa gagaaggaga aagagaagga aaaagaagat gatgaagagg
     2401 aagaggatga agatgcatca gggggcgatc aagatcagga agaaagaaga tggaacaaaa
     2461 ggactcagca gatgcttcat ggtcttcagc gtgctcttgc taaaactgga gctgaatcta
     2521 tcagtttgct tgagttatgt cgaaatacga acagaaaaca agctgccgca aagttctaca
     2581 gcttcttggt tcttaaaaag cagcaagcta ttgagctgac acaggaagaa ccgtacagtg
     2641 acatcatcgc aacacctgga ccaaggttcc atattatata aggtaccgcg ggcccgggat
     2701 ccaccggatc tagataactg atcataatca gccataccac atgtagagta ggcc
//
