LOCUS       sequence-88895-e        6358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 490 / 553, Emission: 507 /
            573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6358)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..6358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..723
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgaaca ccccgggaat taacctgatc
       61 aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg tgaacggcca cgccttcgtg
      121 atcgagggcg agggcaaggg caagccctac gagggcaccc agaccgccaa cctgaccgtg
      181 aaggagggcg cccccctgcc cttcagctac gacatcctga ccaccgccgt gcactacggc
      241 aaccgggtgt tcaccaagta ccccgaggac atccccgact acttcaagca gagcttcccc
      301 gagggctaca gctgggagcg caccatgacc ttcgaggaca agggcatctg caccatccgc
      361 agcgacatca gcctggaggg cgactgcttc ttccagaacg tgcgcttcaa gggcaccaac
      421 ttccccccca acggccccgt gatgcagaag aagaccctga agtgggagcc cagcaccgag
      481 aagctgcacg tgcgcgacgg cctgctggtg ggcaacatca acatggccct gctgctggag
      541 ggcggcggcc actacctgtg cgacttcaag accacctaca aggccaagaa ggtggtgcag
      601 ctgcccgacg cccacttcgt ggaccaccgc atcgagatcc tgggcaacga cagcgactac
      661 aacaaggtga agctgtacga gcacgccgtg gcccgctaca gccccctgcc cagccaggtg
      721 tggtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccttcgat
      781 gctcagcttt tagctgccct tcctaaagtt gcagaattaa gaaaaatctt tgaaccaaag
      841 aggaaagaat ttttagaaat gaaaagaaaa gaaagaattg cccggcgctt agaaggaatt
      901 gaaaccgaca cgcagcccat cctcttgcag agctgcacgg gcttggtgac tcatcgcctg
      961 ctggaggagg acacacccag atacatgcgt gccacagacc cggccagccc gcacattggt
     1021 cgatcaaatg aagaagaaga aacttcagat tcttcactag aaaagcagac tcgatccaaa
     1081 cagtgcacag aaacctcagg catccatgct gactcgccct acagttcagg catcatggac
     1141 acccagagcc tcgagtccaa agccgaaaga atcgccaggt acaaagcaga gagaagacgg
     1201 cagctggcag aaaagtatgg gctcaccctg gatccggaag cagactctga aactccatct
     1261 cgatacagca ggtccagaaa ggaccccgag gctgcggaga aaaggggagt aagaagcgag
     1321 cgatcggccg agtccagcag agacgccggc tcatcctact ccaggactga gctctcgggg
     1381 ctcaggacct gcgtggctga gtccaaggac tanggcctgc accggagtga cggtgtttcc
     1441 gacacagagg tgctgctcaa tgcagaaaac caaagacgcg gtcaagagcc cagtgccacc
     1501 gggctggccc gagacctgcc ccttgcaggg gaggtctcct cttctttctc attctccggg
     1561 cgagactctg ccctcggtga agtgccaagg tccccaaagg cagtgcacag cctgcccagt
     1621 ccatcgcctg ggcagccggc ctccccaagc cactccacca gtgacctgcc actccctgcg
     1681 gaggctcgag ccaggtcaac ttcaaattca gaaatgccag ctgccgagga tgaagaaaag
     1741 gtcgatgagc gagcgaggct gagcgtcgct gctaagaggc tgctcttcag ggagatggag
     1801 aagtcgtttg acgagaagag cgtcccaaag cgccgctccc gcaatgcggc cgtggagcag
     1861 aggctgcggc gcctccagga ccggtcccac acgcagcccg tcaccaccga ggaggtggtc
     1921 attgcagcca cattgcaggc gtctgctcac caaaaggcac tagccagaga ccagacaaac
     1981 gagagcaaag attctgctga gcagggagaa cctgactcct ccactctgag cttagcggag
     2041 aagctggcct tgtttaacaa actatctcag ccggtctcaa aagccatttc aacacggaac
     2101 agattagaca tgagacagag gaggatgaat gctcgttatc aaacccagcc ggtcacgctt
     2161 ggagaagtgg aacaggtgca gagtggcaag ctcatggctt tctctcccac cattaacact
     2221 tccgtgtcca ccgtggcatc taccgtcccc cccatgtatg caggaaatct tcggacaaag
     2281 ccacttccag atgacagctt tggtgccact gagcagaagt ttgcttcttc gctagaaaac
     2341 tccgactccc cagttagaag catcctgaaa tcccaaggct ggcagccctc ggtcgagggt
     2401 gctgggagca aagcaatgtt gagagaattt gaagagacag aacgcaaggg aggcttgaca
     2461 ggtggagatg gcggggttac aaagtatggg tccttcgagg aagcagagct gtcctacccc
     2521 gtcctcagca gagtccggga gggagacaac cataaagagg ccatctatgc ccttccgagg
     2581 aaaggaagcc tggagctcgc ccatcctccc atcgcccagc ttggtgatga cctgaaggaa
     2641 ttttccaccc caaagagcac catgcaggca agcccagact ggaaggagag gcagctcttt
     2701 gaagagaagg tggacttgga gaatgtcaca aagaggaagt tttcgctgaa agccgcggag
     2761 ttcggggaac ccacttctga gcagaccggc gcggctgctg ggaaaccggc tgctccgact
     2821 gcaaccccgg tgtcctggaa gccgcaggat ccctccgaac agcctcagga gaagcggtat
     2881 cagagcccgt gtgcgatgtt tgctgctgga gagatcaaag ccccggcggt ggagggcagc
     2941 ctcgactccc ccagcaaaac catgtccatt aaagaaagat tagcactgtt gaagaagagc
     3001 ggtgaggaag actggagaaa cagactcaac agaaagcagg aatacggcaa agcctccatc
     3061 accagcagcc tgcacatcca agagacggag cagtcactca agaagaagcg ggtcacagaa
     3121 agtcgagaga gccagatgac tattgaagag agaaagcatc tcatcaccgt gagagaggac
     3181 gcctggaaga ccaggggcaa aggagcagcc aacgactcta cccagtttac tgtggccggc
     3241 aggatggtga agcgaggtct ggcgtccccc actgccatca ccccggtagc atcccctgtt
     3301 tccagcaaag caaggggcac tacaccagtt tccagacccc tggaagatat cgaagccaga
     3361 ccagacatgc agttagagtc ggacctcaag ctggacaggc tggaaacctt cctaagaagg
     3421 ctgaataaca aagttggtgg gatgcaagaa acagtcctca ctgtcactgg gaaatcggtg
     3481 aaagaggtga tgaagccgga cgatgatgaa acctttgcca agttttaccg cagcgtggac
     3541 tcctctctac cgagaagccc cgtagagctg gacgaggatt tcgatgtcat ttttgatcct
     3601 tatgccccca ggttgacatc ctcggtggct gagcacaagc gtgccgtgcg gcccaagcgc
     3661 cgggtccagg cttccaaaaa ccccctgaag atgctggccg cgagggaaga tctcctgcag
     3721 gagtacactg agcagagact gaacgtggcc ttcgtggagt caaagcggat gaaagtcgaa
     3781 aagttgtccg ccaactccag cttctcagaa gtcaccctgg cggggttagc cagcaaagaa
     3841 aacttcagca acgtcagcct gcggagcgtc aacctgacgg aacagaattc caacaacagt
     3901 gcagtgccct acaagaagct gatgctgctg caggttaaag gaagaagaca cgtgcagacg
     3961 cggctggtgg agcctcgcgc cccctccctc aacagcgggg actgcttcct cctgctctcg
     4021 ccccatcact gcttcctgtg ggtcggagag ttcgccaacg tgatcgagaa ggcgaaggcc
     4081 tcagaacttg cgagtttaat tcagaccaag agggaacttg gttgtagagc aacttacatc
     4141 cagactgttg aagaaggaat taatacacac acacatgcag ccaaagactt ctggaagctt
     4201 ctgggcggcc aagccagtta ccaatccgcc ggagacccaa aggaggatga gctctatgaa
     4261 acggccataa tagagaccaa ctgcatttac cgtctgatgg atgacaaact cgttcctgat
     4321 gatgactact gggggaagat cccaaagtgc tccctcctgc aatcaaaaga ggtactggtg
     4381 tttgattttg ggagtgaagt ttacgtgtgg catggaaaag aagtcacatt agcacaacgg
     4441 aaaatcgcct ttcagctggc aaagcacttg tggaatggaa cctttgacta cgagaattgt
     4501 gacattaacc cgctggatcc tggggaatgc aatcccctca ttcccaggaa aggacagggg
     4561 cggcccgact gggcaatatt tgggagactt acagaacaca atgagacgat cttgttcaaa
     4621 gagaaattcc ttgattggac ggaactgaag agacctaacg agaagaacgc cagcgaactc
     4681 gcgcagcaca aggacgatgc ccgggcagag gtcaagcctt acgacgtgac gcggatggtg
     4741 cccgtgcccc agacaacagc cggcaccgtg ctggatgggg tcaacgtggg ccgaggctac
     4801 gggctggtgg aaggggacga ccggaggcag ttcgagatcg ccagcatctc agtggacgtc
     4861 tggcacatcc tggagttcga ctacagcagg ctccccaagc agagcatcgg gcagttccac
     4921 gaaggcgatg cctacgtggt gaagtggaag ttcatcgtga gcactgcagt gggaagccgg
     4981 cagaaggggg agcactcggt cagggtggct ggcaaagaga aatgtgtcta cttcttctgg
     5041 caaggccgcc agtcgaccgt gagtgagaag ggcacatcag ctctgatgac agtggagctg
     5101 gacgaggaaa ggggggccca ggtccaggtc ctgcagggca aggagccccc ctgtttcctg
     5161 cagtgcttcc agggggggat ggtggtccac tccgggagac gggaggagga ggaagagaac
     5221 acacagagcg agtggcggct gtactgtgtg cgcggagagg tgcctgtgga ggggaacttg
     5281 ctggaggtgg cctgtcactg cagcagcctc aggtctagga cgtccatggt tgtcctcaac
     5341 gtccacaaag ccctcatcta cctgtggcat ggatgcaaag cccaggccca cacgaaggag
     5401 gtcggacgga ctgcagccaa taaaatcaag gaccaatgtc ccctggaggc cgggctgcac
     5461 agtagcagca aagtgacaat tcatgagtgt gatgaaggct cggagcccct ggggttctgg
     5521 gacgctctgg ggaggagaga ccggaaagcc tacgactgca tgcttcaaga tcctggaaat
     5581 tttaacttca caccccgcct gttcatcctc agcagctcct ctggtgactt ctcagccaca
     5641 gagttcatgt atcccgcccg agacccctct gtggtcaact ctatgccctt cctgcaggaa
     5701 gacctataca gtgccccaca gccagcactc ttccttgttg acaatcacca cgaggtgtac
     5761 ctctggcaag gctggtggcc catcgagaac aagatcacgg gctccgcgcg catccgctgg
     5821 gcctcggacc ggaagagcgc catggagaca gtgctgcagt actgccgagg gaaaaacctg
     5881 aagaagccgc cccccaagtc ttacctcatc cacgctggcc tggaacccct gaccttcacc
     5941 aacatgttcc ccagctggga gcacagagaa gacattgcag agatcacgga gatggacacg
     6001 gaagtttcaa atcagatcac cctagtggag gatgtcttag ccaaactgtg taagaccatt
     6061 tacccactgg ccgacctcct agccaggccg ctccctgagg gagttgaccc tctgaagctg
     6121 gagatctatc tcaccgacga ggacttcgag tttgcactag acatgacccg agacgagtac
     6181 aatgcactgc ccgcttggaa gcaggtgaac ctgaagaaag cgaaaggtct gttctgagtc
     6241 aggagatgct ggaggaacct gggtggtcac tttcaatact gatggaccag gaaaatggat
     6301 gtttaatcga attcccgcgg ccttctagat aactgatcat aatcagccat accacatt
//
