LOCUS       sequence-88902-e        4021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 
            / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: Dendra2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..712
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatgaacac cccgggaatt aacctgatca aggaggacat
       61 gcgcgtgaag gtgcacatgg agggcaacgt gaacggccac gccttcgtga tcgagggcga
      121 gggcaagggc aagccctacg agggcaccca gaccgccaac ctgaccgtga aggagggcgc
      181 ccccctgccc ttcagctacg acatcctgac caccgccgtg cactacggca accgggtgtt
      241 caccaagtac cccgaggaca tccccgacta cttcaagcag agcttccccg agggctacag
      301 ctgggagcgc accatgacct tcgaggacaa gggcatctgc accatccgca gcgacatcag
      361 cctggagggc gactgcttct tccagaacgt gcgcttcaag ggcaccaact tcccccccaa
      421 cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga agctgcacgt
      481 gcgcgacggc ctgctggtgg gcaacatcaa catggccctg ctgctggagg gcggcggcca
      541 ctacctgtgc gacttcaaga ccacctacaa ggccaagaag gtggtgcagc tgcccgacgc
      601 ccacttcgtg gaccaccgca tcgagatcct gggcaacgac agcgactaca acaaggtgaa
      661 gctgtacgag cacgccgtgg cccgctacag ccccctgccc agccaggtgt ggtccggact
      721 cagatctcga gctcaagctt cgaattcggg aaactcgaga acaaagcttg aaaacggccg
      781 catgccagtg tttcatacgc gcacgatcga gagcatcctg gagccggtgg cacagcagat
      841 ctcccacctg gtgataatgc acgaggaggg cgaggtggac ggcaaagcca ttcctgacct
      901 caccgcgccc gtggccgccg tgcaggcggc cgtcagcaac ctcgtccggg ttggaaaaga
      961 gactgttcaa accactgagg atcagatttt gaagagagat atgccaccag catttattaa
     1021 ggttgagaat gcttgcacca agcttgtcca ggcagctcag atgcttcagt cagaccctta
     1081 ctcagtgcct gctcgagatt atctaattga tgggtcaagg ggcatcctct ctggaacatc
     1141 agacctgctc cttaccttcg atgaggctga ggtccgtaaa attattagag tttgcaaagg
     1201 aattttggaa tatcttacag tggcagaggt ggtggagact atggaagatt tggtcactta
     1261 cacaaagaat cttgggccag gaatgactaa gatggccaag atgattgacg agagacagca
     1321 ggagctcact caccaggagc accgagtgat gttggtgaac tcgatgaaca ccgtgaaaga
     1381 gttgctgcca gttctcattt cagctatgaa gatttttgta acaactaaaa actcaaaaaa
     1441 ccaaggcata gaggaagctt taaaaaatcg caattttact gtagaaaaaa tgagtgctga
     1501 aattaatgag ataattcgtg tgttacaact cacctcttgg gatgaagatg cctgggccag
     1561 caaggacact gaagccatga agagagcatt ggcctccata gactccaaac tgaaccaggc
     1621 caaaggttgg ctccgtgacc ctagtgcctc cccaggggat gctggtgagc aggccatcag
     1681 acagatctta gatgaagctg gaaaagttgg tgaactctgt gcaggcaaag aacgcaggga
     1741 gattctggga acttgcaaaa tgctagggca gatgactgat caagtggctg acctccgtgc
     1801 cagaggacaa ggatcctcac cggtggccat gcagaaagct cagcaggtat ctcagggtct
     1861 ggatgtgctc acagcaaaag tggaaaatgc agctcgcaag ctggaagcca tgaccaactc
     1921 aaagcagagc attgcaaaga agatcgatgc tgctcagaac tggcttgcag atccaaatgg
     1981 tggaccggaa ggagaagagc agattcgagg tgctttggct gaagctcgga aaatagcaga
     2041 attatgtgat gatcctaaag aaagagatga cattctacgt tcccttgggg aaatatctgc
     2101 tctgacttct aaattagcag atctacgaag acaggggaaa ggagattctc cagaggctcg
     2161 agccttggcc aaacaggtgg ccacggccct gcagaacctg cagaccaaaa ccaaccgggc
     2221 tgtggccaac agcagaccgg ccaaagcagc tgtacacctt gagggcaaga ttgagcaagc
     2281 acagcggtgg attgataatc ccacagtgga tgaccgtgga gtcggtcagg ctgccatccg
     2341 ggggcttgtg gccgaagggc atcgtctggc taatgttatg atggggcctt atcggcaaga
     2401 tcttctcgcc aagtgtgacc gagtggacca gctgacagcc cagctggctg acctggctgc
     2461 cagaggggaa ggggagagtc ctcaggcacg agcacttgca tctcagctcc aagactcctt
     2521 aaaggatcta aaagctcgga tgcaggaggc catgactcag gaagtgtcag atgttttcag
     2581 cgataccaca actcccatca agctgttggc agtggcagcc acggcgcctc ctgatgcgcc
     2641 taacagggaa gaggtatttg atgagagggc agctaacttt gaaaaccatt caggaaagct
     2701 tggtgctacg gccgagaagg cggctgcggt tggtactgct aataaatcaa cagtggaagg
     2761 cattcaggcc tcagtgaaga cggcccgaga actcacaccc caggtggtct cggctgctcg
     2821 tatcttactt aggaaccctg gaaatcaagc tgcttatgaa cattttgaga ccatgaagaa
     2881 ccagtggatc gataatgttg aaaaaatgac agggctggtg gacgaagcca ttgataccaa
     2941 atctctgttg gatgcttcag aagaagcaat taaaaaagac ctggacaagt gcaaggtagc
     3001 tatggccaac attcagcctc agatgctggt tgctggggca accagtattg ctcgtcgggc
     3061 caaccggatc ctgctggtgg ctaagaggga ggtggagaat tccgaggatc ccaagttccg
     3121 tgaggctgtg aaagctgcct ctgatgaatt gagcaaaacc atctccccga tggtgatgga
     3181 tgcaaaagct gtggctggaa acatttccga ccctggactg caaaagagct tcctggactc
     3241 aggatatcgg atcctgggag ctgtggccaa ggtcagagaa gccttccaac ctcaggagcc
     3301 tgacttcccg ccgcctccac cagaccttga acaactccga ctaacagatg agcttgctcc
     3361 tcccaaacca cctctgcctg aaggtgaggt ccctccacct aggcctccac caccagagga
     3421 aaaggatgaa gagttccctg agcagaaggc cggggaggtg attaaccagc caatgatgat
     3481 ggctgccaga cagctccatg atgaagctcg caaatggtcc agcaagggca atgacatcat
     3541 tgcagcagcc aagcgcatgg ctctgctgat ggctgagatg tctcggctgg taagaggggg
     3601 cagtggtacc aagcgggcac tcattcagtg tgccaaggac atcgccaagg cctcagatga
     3661 ggtgactcgg ttggccaagg aggttgccaa gcagtgcaca gataaacgga ttagaaccaa
     3721 cctcttacag gtatgtgagc gaatcccaac cataagcacc cagctcaaaa tcctgtccac
     3781 agtgaaggcc accatgctgg gccggaccaa catcagtgat gaggagtctg agcaggccac
     3841 agagatgctg gttcacaatg cccagaacct catgcagtct gtgaaggaga ctgtgcggga
     3901 agctgaagct gcttcaatca aaattcgaac agatgctgga tttacactgc gctgggttag
     3961 aaagactccc tggtaccagt aggcggccgc gactctagat cataatcagc cataccacat
     4021 t
//
