LOCUS       sequence-88908-e        4242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 458 / 475, 
            Emission: 458 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4242)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Phamret-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..552)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     protein_bind    3250..3261
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggt
      721 accaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccttca gctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1201 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agccaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     1441 agatctggca gcggtggagg cttggaggag ccggaggccg cgacgcggac agccgcggcc
     1501 gtggattgca aagacagacc cgggttccca gtgaagaggt taatacaagc ccgcttgccc
     1561 ttcaaacgcc tgaaccttgt tcccaaggag aaagttgagg aagacacatc accaaaagca
     1621 gctgtggaaa gcaaagtccc ggatctacaa ttgtctttgg gcacttttga aagccagtgt
     1681 cacacaggct ctcacgtagg cttgagtaca aagttggtcg gtggccaggg ccccattgat
     1741 agcttcttga gggcaacaat caagccagta ccgagtgtgg tcattatcga cctgacggag
     1801 aactgcagtg acattccaga cagccctgag ggccacagtg aactaagtcc tgacactgct
     1861 ggtgtggtca ccacagtgga aggagctgcc aagcagcagg agcactctgc agcagagctg
     1921 tgtctcctag agaccccttc agacatcact tgtcacatgg aagaggagcc tgggagtcca
     1981 ggagacccaa agaggacggg tgactgtcag gctggttcac tacagagctg tccggagctg
     2041 accccaggtt ctaggacatg ccccaccaag gagttatcaa gctggagcaa ggcaggggat
     2101 ctcctattta tagagaaagt gcctgtggtg gtgctagagg acatcctggc cactaagccc
     2161 tccattgcct ctcttcccat gatgtccctg gataggagtg tcacttcaga gagtgaaata
     2221 ctggagtcct gccccgagga tgactccata ctgagccact cctccaccaa ctcctcttct
     2281 ccaaccagct ctcctgaggg accatccaca cccccagaac atcggggtgg gagaagctcc
     2341 ccctccaccc cagcctgcag agttgccaag aactttgtca aaggctccac agagaagggc
     2401 aggagcaaac tgcacagaga tagagaacag cagagggaag agaaggaaaa gctgagggaa
     2461 gagatacgac gagccaagga ggaggcacgg aagaagaagg aggaggagaa ggagctgaag
     2521 gaaaaggagc ggagggaaaa gcgggagaaa gatgagaagg agaaggcgga gaagcagcgg
     2581 ctaaaggagg aaaaacgcaa ggagcggcag gaagctctgg aggctaaact ggaggagaag
     2641 aggaagaagg aggaagagaa gcggttacgg gaggaagaga agcggttacg ggaagaagag
     2701 aagcgcatta aggcagaaaa ggcagagatc actcgattct tccagaaacc gaagactccg
     2761 caggctccca agaccctggc cggttcctgt ggtaagtttg ccccctttga aatcaaagaa
     2821 cacatggtgc tggctccacg gtgccgagct gccttggatc aagacctgtg cgatcagctg
     2881 gaccagctcc tgcagcagca gagtgtggca agcaccttcc tcagtgacct aaaaagccgg
     2941 ctgcccttga gatcggggcc cacccgggtc tgtggccatg acacagacat catgaacagg
     3001 gatgtggtca ttgtggagag cagcaaggtg gacggtgtgt ctgaaaggaa gaagtttggc
     3061 cgcatgaagc ttctgcagtt ctcagagaac caccggccag catactgggg gacgtggaac
     3121 aagaagacag ccatcatccg cccccggaac ccctgggccc aggacaaaga ccttcttgac
     3181 tatgaggtag acagtgacga tgagtgggaa gaggaggaac ctggggagtc tttgtcccac
     3241 agtgaggggg atgaggatga cgatgtgggg gaagatgagg atgaggacga tggcttcttt
     3301 gtgccccatg ggtacttatc tgaagatgaa ggcgtgactg aggagtgtgc agaccctgag
     3361 aaccacaaag tacaccagaa gctgaaggcc aaggagtggg atgagctcct agccaagggc
     3421 aagcgctttc gtgtgctgca gcctgtgcac gtgggatgtg tatgggcatc agaggcagcc
     3481 aactgcacga gttccgactt gaagctgctg cagcagttca ctgcatgcct gctggatgtg
     3541 gcatcccctg atgagccaga acccggggcc tccaggaggg aaaagaggga ccagcatatc
     3601 ctggcccagt tgcttccgct gctacatggc aatgtgaatg ggagcaaggt gatcatccat
     3661 gaattccagg agcaatgccg caggggcctg ctgacccttc caagtcccac cccacacctg
     3721 cagatgccca acttggagga tgctgttgct gtgccatcca aggccaggct gaagcgcctt
     3781 atctctgaga actctgccta tgagaagcgg cccaacttcc gtatgtgctg gtatgtgcac
     3841 ccagaggtgc tcaagagctt tggccaggag tgcctgccag tgccctgcca gtggacctac
     3901 atcaccacca tgccctcagc acccagggaa gacagtggca gtgcttctac tgaggggcct
     3961 ggccagagca cacctatgct actcaagcgg aagccagcgg ccaccatgtg catcacgcag
     4021 tttatgaaga agcggcgcta tgatggacag gttgggtctg gggacatgga tggcttccag
     4081 gcagacacag aagaggatga agaggatgac acagactgta tgatcataga tgtgccagat
     4141 gttgggagtg acgtgtcaga agctcccata cctgctccca cactttgcaa gtgaggatcc
     4201 accggatcta gataactgat cataatcagc cataccacat tt
//
