LOCUS       sequence-88910-e        2946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 458 / 475, 
            Emission: 458 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2946)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Phamret-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..2946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1497..1506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1503..1505,1506..1508,1509..2021)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
ORIGIN
        1 ggacggtcag atccgctagc gccaccatgg gctgtgtgca atgtaaggat aaagaagcaa
       61 caaaactgac ggaggagagg gacggcagcc tgaaccagag ctctgggtac cgctatggca
      121 cagaccccac ccctcagcac taccccagct tcggtgtgac ctccatcccc aactacaaca
      181 acttccacgc agccgggggc caaggactca ccgtctttgg aggtgtgaac tcttcgtctc
      241 atacggggac cttgcgtacg agaggaggaa caggagtgac actctttgtg gccctttatg
      301 actatgaagc acggacagaa gatgacctga gttttcacaa aggagaaaaa tttcaaatat
      361 tgaacagctc ggaaggagat tggtgggaag cccgctcctt gacaactgga gagacaggtt
      421 acattcccag caattatgtg gctccagttg actctatcca ggcagaagag tggtactttg
      481 gaaaacttgg ccgaaaagat gctgagcgac agctattgtc ctttggaaac ccaagaggta
      541 cctttcttat ccgcgagagt gaaaccacca aaggtgccta ttcactttct atccgtgatt
      601 gggatgatat gaaaggagac catgtcaaac attataaaat tcgcaaactt gacaatggtg
      661 gatactacat taccacccgg gcccagtttg aaacacttca gcagcttgta caacattact
      721 caggtacctg gaatggaaac acaaaagtag ccataaagac tcttaaacca ggcacaatgt
      781 cccccgaatc attccttgag gaagcgcaga tcatgaagaa gctgaagcac gacaagctgg
      841 tccagctcta tgcagtggtg tctgaggagc ccatctacat cgtcaccgag tatatgaaca
      901 aaggaagttt actggatttc ttaaaagatg gagaaggaag agctctgaaa ttaccaaatc
      961 ttgtggacat ggcagcacag gtggctgcag gaatggctta catcgagcgc atgaattata
     1021 tccatagaga tctgcgatca gcaaacattc tagtggggaa tggactcata tgcaagattg
     1081 ctgacttcgg attggcccga ttgatagaag acaatgagta cacagcaaga caaggtgcaa
     1141 agttccccat caagtggacg gcccccgagg cagccctgta cgggaggttc acaatcaagt
     1201 ctgacgtgtg gtcttttgga atcttactca cagagctggt caccaaagga agagtgccat
     1261 acccaggcat gaacaaccgg gaggtgctgg agcaggtgga gcgaggctac aggatgccct
     1321 gcccgcagga ctgccccatc tctctgcatg agctcatgat ccactgctgg aaaaaggacc
     1381 ctgaagaacg ccccactttt gagtacttgc agagcttcct ggaagactac tttaccgcga
     1441 cagagcccca gtaccaacct ggtgaaaacc tggcatccag cggggatcca ccggtcgcca
     1501 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1561 acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc gatgccacct
     1621 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1681 ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
     1741 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgtaccatct
     1801 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1861 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1921 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga
     1981 acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     2041 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2101 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     2161 tcctgctgga gttcgtgacc gccgccggta ccaagggcga ggagctgttc accggggtgg
     2221 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2281 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2341 agctgcccgt gccctggccc accctcgtga ccaccttcag ctacggcgtg cagtgcttca
     2401 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2461 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2521 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2581 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2641 tcatggccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2701 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2761 ccgtgctgct gcccgacaac cactacctga gccaccagtc cgccctgagc aaagacccca
     2821 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2881 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     2941 ttgaga
//
