LOCUS       sequence-88912-e        3269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 458 / 475, Emission: 458
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3269)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Phamret-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..3269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..553)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaggttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
      481 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggcccact tcaagatccg
      541 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 taccaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      781 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      841 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      901 gaccaccttc agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      961 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1021 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1081 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1141 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1201 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1261 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1321 gagccaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1381 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     1441 cagatctcga gctcaagctt cgatggcgac tgcgaccccc gtgccgccgc ggatgggcag
     1501 ccgcgctggc ggccccacca cgccgctgag ccccacgcgc ctgtcgcggc tccaggagaa
     1561 ggaggagctg cgcgagctca atgaccggct ggcggtgtac atcgacaagg tgcgcagcct
     1621 ggagacggag aacagcgcgc tgcagctgca ggtgacggag cgcgaggagg tgcgcggccg
     1681 tgagctcacc ggcctcaagg cgctctacga gaccgagctg gccgacgcgc gacgcgcgct
     1741 cgacgacacg gcccgcgagc gcgccaagct gcagatcgag ctgggcaagt gcaaggcgga
     1801 acacgaccag ctgctcctca actatgctaa gaaggaatct gatcttaatg gcgcccagat
     1861 caagcttcga gaatatgaag cagcactcaa ttcgaaagat gcagctcttg ctactgcact
     1921 tggtgacaaa aaaagtttag agggagattt ggaggatctg aaggatcaga ttgcccagtt
     1981 ggaagcctcc ttagctgcag ccaaaaaaca gttagcagat gaaactttac ttaaagtaga
     2041 tttggagaat cgttgtcaga gccttactga ggacttggag tttcgcaaaa gcatgtatga
     2101 agaggagatt aacgagacca gaaggaagca tgaaacgcgc ttggtagagg tggattctgg
     2161 gcgtcaaatt gagtatgagt acaagctggc gcaagccctt catgagatga gagagcaaca
     2221 tgatgcccaa gtgaggctgt ataaggagga gctggagcag acttaccatg ccaaacttga
     2281 gaatgccaga ctgtcatcag agatgaatac ttctactgtc aacagtgcca gggaagaact
     2341 gatggaaagc cgcatgagaa ttgagagcct ttcatcccag ctttctaatc tacagaaaga
     2401 gtctagagca tgtttggaaa ggattcaaga attagaggac ttgcttgcta aagaaaaaga
     2461 caactctcgt cgcatgctga cagacaaaga gagagagatg gcggaaataa gggatcaaat
     2521 gcagcaacag ctgaatgact atgaacagct tcttgatgta aagttagccc tggacatgga
     2581 aatcagtgct tacaggaaac tcttagaagg cgaagaagag aggttgaagc tgtctccaag
     2641 cccttcttcc cgtgtgacag tatcccgagc atcctcaagt cgtagtgtac gtacaactag
     2701 aggaaagcgg aagagggttg atgtggaaga atcagaggcg agtagtagtg ttagcatctc
     2761 tcattccgcc tcagccactg gaaatgtttg catcgaagaa attgatgttg atgggaaatt
     2821 tatccgcttg aagaacactt ctgaacagga tcaaccaatg ggaggctggg agatgatcag
     2881 aaaaattgga gacacatcag tcagttataa atatacctca agatatgtgc tgaaggcagg
     2941 ccagactgtt acaatttggg ctgcaaacgc tggtgtcaca gccagccccc caactgacct
     3001 catctggaag aaccagaact cgtggggcac tggcgaagat gtgaaggtta tattgaaaaa
     3061 ttctcaggga gaggaggttg ctcaaagaag tacagtcttt aaaacaacca tacctgaaga
     3121 agaggaggag gaggaagaag cagctggagt ggttgttgag gaagaacttt tccaccagca
     3181 gggaacccca agagcatcca atagaagctg tgcaattatg tagggatcca ccggatctag
     3241 ataactgatc ataatcagcc ataccacat
//
