LOCUS       sequence-88914-e        4692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 458 / 475, Emission: 458 
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4692)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Phamret-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
ORIGIN
        1 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcggc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgccggta ccaagggcga ggagctgttc
      721 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      781 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcag ctacggcgtg
      901 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      961 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1021 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1081 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1141 aacgtctata tcatggccga caagcagaag aacggcatca aggccaactt caagatccgc
     1201 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1261 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccaccagtc cgccctgagc
     1321 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1381 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     1441 aattcgggaa actcgagaac aaagcttgaa aacggccgca tgccagtgtt tcatacgcgc
     1501 acgatcgaga gcatcctgga gccggtggca cagcagatct cccacctggt gataatgcac
     1561 gaggagggcg aggtggacgg caaagccatt cctgacctca ccgcgcccgt ggccgccgtg
     1621 caggcggccg tcagcaacct cgtccgggtt ggaaaagaga ctgttcaaac cactgaggat
     1681 cagattttga agagagatat gccaccagca tttattaagg ttgagaatgc ttgcaccaag
     1741 cttgtccagg cagctcagat gcttcagtca gacccttact cagtgcctgc tcgagattat
     1801 ctaattgatg ggtcaagggg catcctctct ggaacatcag acctgctcct taccttcgat
     1861 gaggctgagg tccgtaaaat tattagagtt tgcaaaggaa ttttggaata tcttacagtg
     1921 gcagaggtgg tggagactat ggaagatttg gtcacttaca caaagaatct tgggccagga
     1981 atgactaaga tggccaagat gattgacgag agacagcagg agctcactca ccaggagcac
     2041 cgagtgatgt tggtgaactc gatgaacacc gtgaaagagt tgctgccagt tctcatttca
     2101 gctatgaaga tttttgtaac aactaaaaac tcaaaaaacc aaggcataga ggaagcttta
     2161 aaaaatcgca attttactgt agaaaaaatg agtgctgaaa ttaatgagat aattcgtgtg
     2221 ttacaactca cctcttggga tgaagatgcc tgggccagca aggacactga agccatgaag
     2281 agagcattgg cctccataga ctccaaactg aaccaggcca aaggttggct ccgtgaccct
     2341 agtgcctccc caggggatgc tggtgagcag gccatcagac agatcttaga tgaagctgga
     2401 aaagttggtg aactctgtgc aggcaaagaa cgcagggaga ttctgggaac ttgcaaaatg
     2461 ctagggcaga tgactgatca agtggctgac ctccgtgcca gaggacaagg atcctcaccg
     2521 gtggccatgc agaaagctca gcaggtatct cagggtctgg atgtgctcac agcaaaagtg
     2581 gaaaatgcag ctcgcaagct ggaagccatg accaactcaa agcagagcat tgcaaagaag
     2641 atcgatgctg ctcagaactg gcttgcagat ccaaatggtg gaccggaagg agaagagcag
     2701 attcgaggtg ctttggctga agctcggaaa atagcagaat tatgtgatga tcctaaagaa
     2761 agagatgaca ttctacgttc ccttggggaa atatctgctc tgacttctaa attagcagat
     2821 ctacgaagac aggggaaagg agattctcca gaggctcgag ccttggccaa acaggtggcc
     2881 acggccctgc agaacctgca gaccaaaacc aaccgggctg tggccaacag cagaccggcc
     2941 aaagcagctg tacaccttga gggcaagatt gagcaagcac agcggtggat tgataatccc
     3001 acagtggatg accgtggagt cggtcaggct gccatccggg ggcttgtggc cgaagggcat
     3061 cgtctggcta atgttatgat ggggccttat cggcaagatc ttctcgccaa gtgtgaccga
     3121 gtggaccagc tgacagccca gctggctgac ctggctgcca gaggggaagg ggagagtcct
     3181 caggcacgag cacttgcatc tcagctccaa gactccttaa aggatctaaa agctcggatg
     3241 caggaggcca tgactcagga agtgtcagat gttttcagcg ataccacaac tcccatcaag
     3301 ctgttggcag tggcagccac ggcgcctcct gatgcgccta acagggaaga ggtatttgat
     3361 gagagggcag ctaactttga aaaccattca ggaaagcttg gtgctacggc cgagaaggcg
     3421 gctgcggttg gtactgctaa taaatcaaca gtggaaggca ttcaggcctc agtgaagacg
     3481 gcccgagaac tcacacccca ggtggtctcg gctgctcgta tcttacttag gaaccctgga
     3541 aatcaagctg cttatgaaca ttttgagacc atgaagaacc agtggatcga taatgttgaa
     3601 aaaatgacag ggctggtgga cgaagccatt gataccaaat ctctgttgga tgcttcagaa
     3661 gaagcaatta aaaaagacct ggacaagtgc aaggtagcta tggccaacat tcagcctcag
     3721 atgctggttg ctggggcaac cagtattgct cgtcgggcca accggatcct gctggtggct
     3781 aagagggagg tggagaattc cgaggatccc aagttccgtg aggctgtgaa agctgcctct
     3841 gatgaattga gcaaaaccat ctccccgatg gtgatggatg caaaagctgt ggctggaaac
     3901 atttccgacc ctggactgca aaagagcttc ctggactcag gatatcggat cctgggagct
     3961 gtggccaagg tcagagaagc cttccaacct caggagcctg acttcccgcc gcctccacca
     4021 gaccttgaac aactccgact aacagatgag cttgctcctc ccaaaccacc tctgcctgaa
     4081 ggtgaggtcc ctccacctag gcctccacca ccagaggaaa aggatgaaga gttccctgag
     4141 cagaaggccg gggaggtgat taaccagcca atgatgatgg ctgccagaca gctccatgat
     4201 gaagctcgca aatggtccag caagggcaat gacatcattg cagcagccaa gcgcatggct
     4261 ctgctgatgg ctgagatgtc tcggctggta agagggggca gtggtaccaa gcgggcactc
     4321 attcagtgtg ccaaggacat cgccaaggcc tcagatgagg tgactcggtt ggccaaggag
     4381 gttgccaagc agtgcacaga taaacggatt agaaccaacc tcttacaggt atgtgagcga
     4441 atcccaacca taagcaccca gctcaaaatc ctgtccacag tgaaggccac catgctgggc
     4501 cggaccaaca tcagtgatga ggagtctgag caggccacag agatgctggt tcacaatgcc
     4561 cagaacctca tgcagtctgt gaaggagact gtgcgggaag ctgaagctgc ttcaatcaaa
     4621 attcgaacag atgctggatt tacactgcgc tgggttagaa agactccctg gtaccagtag
     4681 gcggccgcga ct
//
